Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           19     869
NP_000170                                                              19     869
NP_001268422                                                           19     869
NP_001268423                                                           19     869
XP_005264328                                                           19     869
XP_006726469                                                           17    2528
XP_006713516                                                           17    2528
NP_001073291                                                           16    5631
NP_000417                                                              16    5631
NP_001158268                                                           16    5631
NP_065782                                                              16    5631
NP_001229341                                                           16    5631
NP_775742                                                              16    5631
XP_005262360                                                           16    5631
XP_005267039                                                           16    5631
XP_005267038                                                           16    5631
XP_006713019                                                           16    5631
NP_005877                                                              15    9605
XP_006721186                                                           15    9605
XP_005255829                                                           15    9605
XP_006721185                                                           15    9605
XP_006721184                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LGASDRTMS 9
            LGASDR MS
Sbjct: 1047 LGASDRIMS 1055
>NP_000170
          Length = 1360

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LGASDRTMS 9
            LGASDR MS
Sbjct: 1177 LGASDRIMS 1185
>NP_001268422
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LGASDRTMS 9
           LGASDR MS
Sbjct: 875 LGASDRIMS 883
>NP_001268423
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LGASDRTMS 9
           LGASDR MS
Sbjct: 875 LGASDRIMS 883
>XP_005264328
          Length = 1261

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LGASDRTMS 9
            LGASDR MS
Sbjct: 1078 LGASDRIMS 1086
>XP_006726469
          Length = 175

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LGASDRTMS 9
           +GA DRT+S
Sbjct: 161 VGAEDRTLS 169
>XP_006713516
          Length = 263

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LGASDRTMS 9
           +GA DRT+S
Sbjct: 249 VGAEDRTLS 257
>NP_001073291
          Length = 3118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    GASDRTMS 9
            GAS+RT+S
Sbjct: 2861 GASNRTIS 2868
>NP_000417
          Length = 3122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    GASDRTMS 9
            GAS+RT+S
Sbjct: 2865 GASNRTIS 2872
>NP_001158268
          Length = 186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LGASDRTMS 9
           LGA DRT S
Sbjct: 168 LGAYDRTHS 176
>NP_065782
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LGASDRT 7
           LGASDR+
Sbjct: 577 LGASDRS 583
>NP_001229341
          Length = 701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LGASDRT 7
           LGASDR+
Sbjct: 559 LGASDRS 565
>NP_775742
          Length = 274

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LGASDRTMS 9
           LGA DRT S
Sbjct: 256 LGAYDRTHS 264
>XP_005262360
          Length = 557

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LGASDRTMS 9
           LGA DR +S
Sbjct: 549 LGAQDRLLS 557
>XP_005267039
          Length = 3206

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    GASDRTMS 9
            GAS+RT+S
Sbjct: 2949 GASNRTIS 2956
>XP_005267038
          Length = 3210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    GASDRTMS 9
            GAS+RT+S
Sbjct: 2953 GASNRTIS 2960
>XP_006713019
          Length = 259

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LGASDRTMS 9
           LGA DRT S
Sbjct: 241 LGAYDRTHS 249
>NP_005877
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GASDRTM 8
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721186
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GASDRTM 8
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_005255829
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GASDRTM 8
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721185
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GASDRTM 8
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721184
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GASDRTM 8
           G+SDRT+
Sbjct: 207 GSSDRTI 213
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.118    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 426,426
Number of extensions: 516
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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