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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           20     509
NP_001257437                                                           20     509
NP_006281                                                              20     509
XP_006715618                                                           20     509
XP_005267176                                                           20     509
NP_387450                                                              17    2528
NP_004352                                                              17    2528
NP_001272369                                                           17    3301
NP_689969                                                              17    3301
NP_001272368                                                           17    3301
XP_005248335                                                           17    3301
XP_006714526                                                           17    3301
NP_002829                                                              16    5631
XP_006711535                                                           16    5631
NP_002205                                                              16    7355
NP_001034553                                                           16    7355
NP_001244068                                                           16    7355
NP_001099003                                                           16    7355
NP_079189                                                              16    7355
NP_004061                                                              16    7355
XP_005249809                                                           16    7355
XP_005249808                                                           16    7355
XP_006710134                                                           16    7355
NP_001036169                                                           16    7355
NP_054706                                                              15    9605
NP_003364                                                              15    9605
NP_663729                                                              15    9605
NP_055835                                                              15    9605
NP_001275932                                                           15    9605
XP_005270200                                                           15    9605
XP_005270199                                                           15    9605

>NP_001257436
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFSETTTMK 9
           LFSETT MK
Sbjct: 469 LFSETTAMK 477



>NP_001257437
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFSETTTMK 9
           LFSETT MK
Sbjct: 469 LFSETTAMK 477



>NP_006281
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFSETTTMK 9
           LFSETT MK
Sbjct: 469 LFSETTAMK 477



>XP_006715618
          Length = 577

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFSETTTMK 9
           LFSETT MK
Sbjct: 256 LFSETTAMK 264



>XP_005267176
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFSETTTMK 9
           LFSETT MK
Sbjct: 469 LFSETTAMK 477



>NP_387450
          Length = 785

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FSETTTMK 9
           FS+TTT+K
Sbjct: 358 FSDTTTVK 365



>NP_004352
          Length = 785

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FSETTTMK 9
           FS+TTT+K
Sbjct: 358 FSDTTTVK 365



>NP_001272369
          Length = 1385

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFSETTTMK 9
            LF++T TMK
Sbjct: 1074 LFTDTITMK 1082



>NP_689969
          Length = 1708

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFSETTTMK 9
            LF++T TMK
Sbjct: 1359 LFTDTITMK 1367



>NP_001272368
          Length = 1732

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFSETTTMK 9
            LF++T TMK
Sbjct: 1359 LFTDTITMK 1367



>XP_005248335
          Length = 1423

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFSETTTMK 9
            LF++T TMK
Sbjct: 1074 LFTDTITMK 1082



>XP_006714526
          Length = 1670

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFSETTTMK 9
            LF++T TMK
Sbjct: 1359 LFTDTITMK 1367



>NP_002829
          Length = 1306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FSETTT 7
          FSETTT
Sbjct: 69 FSETTT 74



>XP_006711535
          Length = 1258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FSETTT 7
          FSETTT
Sbjct: 69 FSETTT 74



>NP_002205
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LFSETTTMK 9
           LF+ T TMK
Sbjct: 429 LFNVTVTMK 437



>NP_001034553
          Length = 1323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LFSETTTM 8
           LFS+T+T+
Sbjct: 344 LFSDTSTL 351



>NP_001244068
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFSETT 6
           LFSETT
Sbjct: 592 LFSETT 597



>NP_001099003
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LFSETTTM 8
           +FSET+T+
Sbjct: 340 VFSETSTL 347



>NP_079189
          Length = 1026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LFSETTTM 8
           +FSET+T+
Sbjct: 340 VFSETSTL 347



>NP_004061
          Length = 687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFSETT 6
           LFSETT
Sbjct: 635 LFSETT 640



>XP_005249809
          Length = 634

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LFSETTTMK 9
           LF+ T TMK
Sbjct: 294 LFNVTVTMK 302



>XP_005249808
          Length = 634

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LFSETTTMK 9
           LF+ T TMK
Sbjct: 294 LFNVTVTMK 302



>XP_006710134
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFSETT 6
           LFSETT
Sbjct: 282 LFSETT 287



>NP_001036169
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFSETT 6
           LFSETT
Sbjct: 634 LFSETT 639



>NP_054706
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LFSETTT 7
           +FS+TTT
Sbjct: 598 VFSDTTT 604



>NP_003364
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LFSETTT 7
           +FS+TTT
Sbjct: 598 VFSDTTT 604



>NP_663729
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFSETTTMK 9
           L SE+TT+K
Sbjct: 275 LCSESTTVK 283



>NP_055835
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LFSETTTMK 9
           +FS  TT+K
Sbjct: 758 IFSHITTLK 766



>NP_001275932
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LFSETTTMK 9
           +FS  TT+K
Sbjct: 691 IFSHITTLK 699



>XP_005270200
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LFSETTT 7
           +FS+TTT
Sbjct: 599 VFSDTTT 605



>XP_005270199
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LFSETTT 7
           +FS+TTT
Sbjct: 599 VFSDTTT 605



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.116    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 410,531
Number of extensions: 816
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)