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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_848594                                                              17    4312
XP_005268803                                                           17    4312
XP_005268802                                                           17    4312
XP_005268801                                                           17    4312
XP_005268800                                                           17    4312
XP_005268799                                                           17    4312
XP_006719330                                                           17    4312
XP_005253911                                                           17    4312
XP_006719329                                                           17    4312
XP_006719328                                                           17    4312
NP_056090                                                              17    4312
NP_002542                                                              16    5631
NP_036505                                                              16    5631
NP_001229510                                                           16    7355
NP_061866                                                              16    7355
XP_005260790                                                           16    7355
XP_005260787                                                           16    7355
XP_005260789                                                           16    7355
XP_006723640                                                           16    7355
XP_006723639                                                           16    7355
XP_005260788                                                           16    7355
XP_006723638                                                           16    7355
XP_006723637                                                           16    7355
XP_006723636                                                           16    7355
NP_062551                                                              16    7355
NP_958441                                                              15    9605
NP_958439                                                              15    9605
NP_958440                                                              15    9605
NP_005219                                                              15    9605
NP_061897                                                              15    9605
XP_005264436                                                           15    9605
XP_006712098                                                           15    9605
XP_006712097                                                           15    9605

>NP_848594
          Length = 550

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFMSLQKMM 9
           L+M+LQK++
Sbjct: 292 LYMNLQKLL 300



>XP_005268803
          Length = 851

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           L+MS+QK M
Sbjct: 460 LYMSMQKPM 468



>XP_005268802
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           L+MS+QK M
Sbjct: 461 LYMSMQKPM 469



>XP_005268801
          Length = 985

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           L+MS+QK M
Sbjct: 272 LYMSMQKPM 280



>XP_005268800
          Length = 986

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           L+MS+QK M
Sbjct: 273 LYMSMQKPM 281



>XP_005268799
          Length = 1174

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           L+MS+QK M
Sbjct: 461 LYMSMQKPM 469



>XP_006719330
          Length = 419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFMSLQKMM 9
           L+M+LQK++
Sbjct: 292 LYMNLQKLL 300



>XP_005253911
          Length = 421

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFMSLQKMM 9
           L+M+LQK++
Sbjct: 292 LYMNLQKLL 300



>XP_006719329
          Length = 506

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFMSLQKMM 9
           L+M+LQK++
Sbjct: 292 LYMNLQKLL 300



>XP_006719328
          Length = 526

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFMSLQKMM 9
           L+M+LQK++
Sbjct: 292 LYMNLQKLL 300



>NP_056090
          Length = 1173

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           L+MS+QK M
Sbjct: 460 LYMSMQKPM 468



>NP_002542
          Length = 321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 MSLQKMM 9
         MSLQK+M
Sbjct: 1 MSLQKLM 7



>NP_036505
          Length = 321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 MSLQKMM 9
         MSLQK+M
Sbjct: 1 MSLQKLM 7



>NP_001229510
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 707 FMTMQKM 713



>NP_061866
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_005260790
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 452 FMTMQKM 458



>XP_005260787
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 624 FMTMQKM 630



>XP_005260789
          Length = 850

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 613 FMTMQKM 619



>XP_006723640
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723639
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_005260788
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723638
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723637
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 658 FMTMQKM 664



>XP_006723636
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FMSLQKM 8
           FM++QKM
Sbjct: 707 FMTMQKM 713



>NP_062551
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LFMSLQKM 8
           L+MSL+K+
Sbjct: 109 LYMSLEKL 116



>NP_958441
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FMSLQKM 8
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_958439
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FMSLQKM 8
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_958440
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FMSLQKM 8
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_005219
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FMSLQKM 8
          F+SLQ+M
Sbjct: 48 FLSLQRM 54



>NP_061897
          Length = 2071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           + MSLQK++
Sbjct: 156 ILMSLQKVL 164



>XP_005264436
          Length = 1881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           + MSLQK++
Sbjct: 156 ILMSLQKVL 164



>XP_006712098
          Length = 2059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           + MSLQK++
Sbjct: 144 ILMSLQKVL 152



>XP_006712097
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LFMSLQKMM 9
           + MSLQK++
Sbjct: 156 ILMSLQKVL 164



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.351    0.140    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 303,859
Number of extensions: 270
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)