Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001240781                                                           17    3301
NP_006585                                                              17    3301
NP_001240782                                                           17    3301
XP_005270439                                                           17    3301
XP_005270438                                                           17    3301
NP_001138767                                                           16    5631
XP_005265374                                                           16    5631
NP_001026                                                              16    7355
XP_005265906                                                           16    7355
XP_006714896                                                           16    7355
XP_006711873                                                           16    7355
XP_006711872                                                           16    7355
XP_006711871                                                           16    7355
XP_006711870                                                           16    7355
XP_006711869                                                           16    7355
XP_006711868                                                           16    7355
XP_006711867                                                           16    7355
XP_006711866                                                           16    7355
XP_006711865                                                           16    7355
NP_079429                                                              16    7355
NP_001006623                                                           15    9605
NP_997293                                                              15    9605
>NP_001240781
          Length = 739

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           LFLI+ K+F
Sbjct: 258 LFLILAKMF 266
>NP_006585
          Length = 739

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           LFLI+ K+F
Sbjct: 258 LFLILAKMF 266
>NP_001240782
          Length = 640

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           LFLI+ K+F
Sbjct: 159 LFLILAKMF 167
>XP_005270439
          Length = 405

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           LFLI+ K+F
Sbjct: 258 LFLILAKMF 266
>XP_005270438
          Length = 635

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           LFLI+ K+F
Sbjct: 258 LFLILAKMF 266
>NP_001138767
          Length = 558

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           +FL+ +KLF
Sbjct: 546 IFLVPLKLF 554
>XP_005265374
          Length = 503

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LFLIMIKLF 9
           +FL+ +KLF
Sbjct: 491 IFLVPLKLF 499
>NP_001026
          Length = 4967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1817 LFVPLIKLF 1825
>XP_005265906
          Length = 1421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LFLIMIK 7
          LF+IMIK
Sbjct: 86 LFVIMIK 92
>XP_006714896
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LFLIMIK 7
           LF+IMIK
Sbjct: 126 LFVIMIK 132
>XP_006711873
          Length = 4954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1816 LFVPLIKLF 1824
>XP_006711872
          Length = 2735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1827 LFVPLIKLF 1835
>XP_006711871
          Length = 4906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1827 LFVPLIKLF 1835
>XP_006711870
          Length = 4965

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1827 LFVPLIKLF 1835
>XP_006711869
          Length = 4973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1827 LFVPLIKLF 1835
>XP_006711868
          Length = 4975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1817 LFVPLIKLF 1825
>XP_006711867
          Length = 4977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1827 LFVPLIKLF 1835
>XP_006711866
          Length = 4984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1826 LFVPLIKLF 1834
>XP_006711865
          Length = 4985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LFLIMIKLF 9
            LF+ +IKLF
Sbjct: 1827 LFVPLIKLF 1835
>NP_079429
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LFLIMIK 7
           LF+IMIK
Sbjct: 126 LFVIMIK 132
>NP_001006623
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   LIMIKLF 9
           ++M+KLF
Sbjct: 221 IVMVKLF 227
>NP_997293
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFLIMIKL 8
           L L+M+KL
Sbjct: 366 LLLVMVKL 373
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.371    0.183    0.508 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 284,271
Number of extensions: 321
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package