Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_001137251                                                           17    4312
NP_054774                                                              17    4312
NP_001391                                                              16    5631
NP_789744                                                              16    5631
NP_036459                                                              16    5631
XP_006710462                                                           16    5631
NP_005468                                                              16    7355
NP_060656                                                              16    7355
NP_113598                                                              16    7355
NP_114037                                                              16    7355
NP_114036                                                              16    7355
NP_114067                                                              16    7355
NP_690605                                                              16    7355
NP_061723                                                              16    7355
NP_061728                                                              16    7355
NP_061730                                                              16    7355
NP_001273926                                                           16    7355
NP_068769                                                              16    7355
NP_061732                                                              16    7355
NP_061725                                                              16    7355
NP_113599                                                              16    7355
NP_113688                                                              16    7355
NP_113683                                                              16    7355
XP_005262778                                                           16    7355
XP_005245374                                                           16    7355
XP_006710337                                                           16    7355
NP_660287                                                              16    7355
NP_114062                                                              16    7355
NP_061734                                                              16    7355
NP_114040                                                              16    7355
NP_061733                                                              16    7355
NP_113689                                                              16    7355
NP_113684                                                              16    7355
NP_061729                                                              16    7355
NP_113685                                                              16    7355
NP_061731                                                              16    7355
NP_001013421                                                           15    9605
NP_001171705                                                           15    9605
NP_001171702                                                           15    9605
NP_001171703                                                           15    9605
NP_001171704                                                           15    9605
XP_005261497                                                           15    9605
XP_005261496                                                           15    9605
XP_006724258                                                           15    9605
NP_066938                                                              15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LFKRLYSL 8
            LFKRLYSL
Sbjct: 1059 LFKRLYSL 1066
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LFKRLYSL 8
            LFKRLYSL
Sbjct: 1059 LFKRLYSL 1066
>NP_001137251
          Length = 676

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFKRLYSLV 9
           + K+LYSLV
Sbjct: 489 MVKKLYSLV 497
>NP_054774
          Length = 676

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFKRLYSLV 9
           + K+LYSLV
Sbjct: 489 MVKKLYSLV 497
>NP_001391
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFKRLYSLV 9
           L+ R+YSLV
Sbjct: 220 LYCRIYSLV 228
>NP_789744
          Length = 931

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFKRLYSLV 9
           LF+RL+S++
Sbjct: 706 LFQRLFSIL 714
>NP_036459
          Length = 931

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LFKRLYSLV 9
           LF+RL+S++
Sbjct: 706 LFQRLFSIL 714
>XP_006710462
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFKRLYSLV 9
           L+ R+YSLV
Sbjct: 220 LYCRIYSLV 228
>NP_005468
          Length = 1203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KRLYSL 8
          KRLYSL
Sbjct: 10 KRLYSL 15
>NP_060656
          Length = 853

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LFKRLYSL 8
           LFK  YSL
Sbjct: 124 LFKEAYSL 131
>NP_113598
          Length = 807

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_114037
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_114036
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_114067
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 400 FKNYYSLV 407
>NP_690605
          Length = 297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LFKRLYSLV 9
          LF+R+ SLV
Sbjct: 30 LFRRMSSLV 38
>NP_061723
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061728
          Length = 948

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061730
          Length = 947

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_001273926
          Length = 102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FKRLYSLV 9
          FK+LY+L+
Sbjct: 19 FKQLYTLM 26
>NP_068769
          Length = 297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LFKRLYSLV 9
          LF+R+ SLV
Sbjct: 30 LFRRMSSLV 38
>NP_061732
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061725
          Length = 949

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 400 FKNYYSLV 407
>NP_113599
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_113688
          Length = 798

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_113683
          Length = 824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>XP_005262778
          Length = 193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KRLYSLV 9
           K+LYSL+
Sbjct: 179 KKLYSLI 185
>XP_005245374
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LFKRLYSL 8
           LFK  YSL
Sbjct: 182 LFKEAYSL 189
>XP_006710337
          Length = 102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FKRLYSLV 9
          FK+LY+L+
Sbjct: 19 FKQLYTLM 26
>NP_660287
          Length = 193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KRLYSLV 9
           K+LYSL+
Sbjct: 179 KKLYSLI 185
>NP_114062
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061734
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_114040
          Length = 789

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061733
          Length = 937

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_113689
          Length = 816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 400 FKNYYSLV 407
>NP_113684
          Length = 808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061729
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_113685
          Length = 824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 401 FKNYYSLV 408
>NP_061731
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKRLYSLV 9
           FK  YSLV
Sbjct: 400 FKNYYSLV 407
>NP_001013421
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LFKRLYS 7
           LFK LYS
Sbjct: 212 LFKPLYS 218
>NP_001171705
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 822 LYKRLY 827
>NP_001171702
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 886 LYKRLY 891
>NP_001171703
          Length = 1013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 886 LYKRLY 891
>NP_001171704
          Length = 1011

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 886 LYKRLY 891
>XP_005261497
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 822 LYKRLY 827
>XP_005261496
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 886 LYKRLY 891
>XP_006724258
          Length = 1022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 886 LYKRLY 891
>NP_066938
          Length = 1024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LFKRLY 6
           L+KRLY
Sbjct: 886 LYKRLY 891
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.157    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 480,574
Number of extensions: 914
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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