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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115894                                                              17    2528
XP_005264828                                                           17    2528
NP_114161                                                              17    4312
XP_006713841                                                           17    4312
NP_443142                                                              16    7355
NP_001268432                                                           16    7355
NP_852004                                                              16    7355
NP_001193795                                                           16    7355
NP_000556                                                              16    7355
NP_056271                                                              16    7355
NP_006868                                                              16    7355
NP_001012999                                                           16    7355
NP_001988                                                              16    7355
XP_005245197                                                           16    7355
XP_005245196                                                           16    7355
XP_006711362                                                           16    7355
XP_006711361                                                           16    7355
NP_006653                                                              15    9605
NP_057331                                                              15    9605
NP_671488                                                              15    9605
NP_001186492                                                           15    9605
NP_057445                                                              15    9605
NP_075048                                                              15    9605
NP_009004                                                              15    9605
XP_006725824                                                           15    9605
XP_006713077                                                           15    9605
XP_005264994                                                           15    9605
XP_006711427                                                           15    9605
XP_005277457                                                           15    9605
XP_006711426                                                           15    9605
NP_699162                                                              15    9605
NP_001121686                                                           15    9605

>NP_115894
          Length = 601

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LFIRSTQV 8
           LF+R+TQV
Sbjct: 536 LFVRATQV 543



>XP_005264828
          Length = 524

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LFIRSTQV 8
           LF+R+TQV
Sbjct: 459 LFVRATQV 466



>NP_114161
          Length = 569

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FIRSTQVH 9
           FIRSTQ++
Sbjct: 439 FIRSTQLN 446



>XP_006713841
          Length = 540

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FIRSTQVH 9
           FIRSTQ++
Sbjct: 439 FIRSTQLN 446



>NP_443142
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LFIRSTQVH 9
           LF+R  ++H
Sbjct: 380 LFLRDNKIH 388



>NP_001268432
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LFIRSTQVH 9
           LF+R  ++H
Sbjct: 380 LFLRDNKIH 388



>NP_852004
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>NP_001193795
          Length = 352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>NP_000556
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>NP_056271
          Length = 580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LFIRSTQVH 9
           LF+R+  VH
Sbjct: 225 LFLRTRNVH 233



>NP_006868
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FIRSTQVH 9
           FIR TQ H
Sbjct: 333 FIRVTQQH 340



>NP_001012999
          Length = 967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIRSTQVH 9
           FIR +++H
Sbjct: 479 FIRKSEIH 486



>NP_001988
          Length = 133

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  LFIRSTQVH 9
          LF+R+ ++H
Sbjct: 3  LFVRAQELH 11



>XP_005245197
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>XP_005245196
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>XP_006711362
          Length = 381

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>XP_006711361
          Length = 484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LFIRSTQVH 9
          L +RS Q+H
Sbjct: 81 LLLRSVQLH 89



>NP_006653
          Length = 3230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LFIRSTQVH 9
           L +R+T VH
Sbjct: 928 LVLRATDVH 936



>NP_057331
          Length = 1241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    FIRSTQVH 9
            FIR+ Q H
Sbjct: 1123 FIRTLQAH 1130



>NP_671488
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RSTQVH 9
           RSTQ+H
Sbjct: 205 RSTQMH 210



>NP_001186492
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RSTQVH 9
           RSTQ+H
Sbjct: 205 RSTQMH 210



>NP_057445
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFIRSTQV 8
           +FIRST +
Sbjct: 159 VFIRSTNI 166



>NP_075048
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   FIRSTQVH 9
           F++ST+ H
Sbjct: 392 FVKSTEPH 399



>NP_009004
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RSTQVH 9
           RSTQ+H
Sbjct: 205 RSTQMH 210



>XP_006725824
          Length = 369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IRSTQVH 9
           IR+TQ H
Sbjct: 107 IRTTQAH 113



>XP_006713077
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IRSTQVH 9
           IR+T+VH
Sbjct: 205 IRNTRVH 211



>XP_005264994
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IRSTQVH 9
           IR+T+VH
Sbjct: 205 IRNTRVH 211



>XP_006711427
          Length = 228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFIRSTQV 8
           +FIRST +
Sbjct: 116 VFIRSTNI 123



>XP_005277457
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFIRSTQV 8
           +FIRST +
Sbjct: 159 VFIRSTNI 166



>XP_006711426
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFIRSTQV 8
           +FIRST +
Sbjct: 116 VFIRSTNI 123



>NP_699162
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IRSTQVH 9
           IR+T+VH
Sbjct: 205 IRNTRVH 211



>NP_001121686
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IRSTQVH 9
           IR+T+VH
Sbjct: 205 IRNTRVH 211



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.142    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,481
Number of extensions: 777
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)