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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_002408                                                              16    5631
NP_001139438                                                           16    5631
XP_006717927                                                           16    5631
NP_944494                                                              16    7355
NP_000807                                                              16    7355
NP_944493                                                              16    7355
NP_006316                                                              16    7355
XP_005253649                                                           16    7355
NP_078905                                                              15    9605
XP_005263275                                                           15    9605

>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEVVKTTA 8
            LEVVKTTA
Sbjct: 1736 LEVVKTTA 1743



>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEVVKTTA 8
            LEVVKTTA
Sbjct: 1736 LEVVKTTA 1743



>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEVVKTTA 8
            LEVVKTTA
Sbjct: 1689 LEVVKTTA 1696



>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEVVKTTA 8
            LEVVKTTA
Sbjct: 1768 LEVVKTTA 1775



>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEVVKTTA 8
            LEVVKTTA
Sbjct: 1768 LEVVKTTA 1775



>NP_002408
          Length = 3256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LEVVKTTA 8
            LEVV TTA
Sbjct: 2711 LEVVDTTA 2718



>NP_001139438
          Length = 2896

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LEVVKTTA 8
            LEVV TTA
Sbjct: 2351 LEVVDTTA 2358



>XP_006717927
          Length = 2406

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    LEVVKTTA 8
            LEVV TTA
Sbjct: 1861 LEVVDTTA 1868



>NP_944494
          Length = 475

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVVKTTA 8
           EVVKTT+
Sbjct: 250 EVVKTTS 256



>NP_000807
          Length = 467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVVKTTA 8
           EVVKTT+
Sbjct: 250 EVVKTTS 256



>NP_944493
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVVKTTA 8
           EVVKTT+
Sbjct: 290 EVVKTTS 296



>NP_006316
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVVKTTAV 9
           LEV +TTA+
Sbjct: 201 LEVAQTTAL 209



>XP_005253649
          Length = 128

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVVKTTAV 9
           LEV +TTA+
Sbjct: 113 LEVAQTTAL 121



>NP_078905
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EVVKTTA 8
           EV++TTA
Sbjct: 163 EVIRTTA 169



>XP_005263275
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EVVKTTA 8
           EV++TTA
Sbjct: 134 EVIRTTA 140



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.121    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 252,572
Number of extensions: 277
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)