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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              19     869
XP_005268094                                                           19     869
NP_705872                                                              16    7355
NP_705871                                                              16    7355
NP_065961                                                              16    7355
NP_001158137                                                           16    7355
NP_039229                                                              16    7355
XP_005254746                                                           16    7355
XP_005254745                                                           16    7355
XP_005254744                                                           16    7355
XP_005254743                                                           16    7355
XP_005254742                                                           16    7355
XP_006716134                                                           16    7355

>NP_000360
          Length = 764

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEVSQTSVT 9
           L+VSQTSVT
Sbjct: 231 LDVSQTSVT 239



>XP_005268094
          Length = 764

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEVSQTSVT 9
           L+VSQTSVT
Sbjct: 231 LDVSQTSVT 239



>NP_705872
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 571 MEVSSSSVT 579



>NP_705871
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 590 MEVSSSSVT 598



>NP_065961
          Length = 1934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEVSQTSV 8
           +E+S+TSV
Sbjct: 498 MEISETSV 505



>NP_001158137
          Length = 1950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEVSQTSV 8
           +E+S+TSV
Sbjct: 498 MEISETSV 505



>NP_039229
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LEVSQTSVT 9
          LE+  TSVT
Sbjct: 68 LEIGYTSVT 76



>XP_005254746
          Length = 1054

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 571 MEVSSSSVT 579



>XP_005254745
          Length = 1067

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 584 MEVSSSSVT 592



>XP_005254744
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 590 MEVSSSSVT 598



>XP_005254743
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 603 MEVSSSSVT 611



>XP_005254742
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVSQTSVT 9
           +EVS +SVT
Sbjct: 603 MEVSSSSVT 611



>XP_006716134
          Length = 1847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEVSQTSV 8
           +E+S+TSV
Sbjct: 422 MEISETSV 429



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.104    0.222 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 214,019
Number of extensions: 307
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)