Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055325                                                              18    1135
NP_149015                                                              18    1135
XP_006715989                                                           18    1135
NP_001245210                                                           18    1482
NP_000242                                                              18    1482
XP_005264389                                                           18    1482
NP_001243000                                                           16    5631
XP_005257603                                                           16    5631
XP_006722058                                                           16    5631
XP_005257602                                                           16    5631
NP_001159923                                                           16    7355
NP_000884                                                              16    7355
NP_001161846                                                           16    7355
NP_001161849                                                           16    7355
NP_055624                                                              16    7355
NP_001258468                                                           16    7355
NP_061897                                                              16    7355
NP_001161847                                                           16    7355
XP_006723717                                                           16    7355
XP_006723715                                                           16    7355
XP_006723712                                                           16    7355
XP_006723711                                                           16    7355
XP_006723710                                                           16    7355
XP_006723709                                                           16    7355
XP_006715350                                                           16    7355
XP_006715349                                                           16    7355
XP_005249561                                                           16    7355
XP_005264436                                                           16    7355
XP_006712098                                                           16    7355
XP_006712097                                                           16    7355
NP_000375                                                              15    9605
NP_001136272                                                           15    9605
NP_001008212                                                           15    9605
NP_068815                                                              15    9605
NP_001008214                                                           15    9605
NP_001008213                                                           15    9605
NP_001180211                                                           15    9605
XP_005252395                                                           15    9605
XP_005252394                                                           15    9605
XP_005252393                                                           15    9605
XP_006713948                                                           15    9605
>NP_055325
          Length = 4935

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1    LETTSILRS 9
            LETT++LRS
Sbjct: 2462 LETTAVLRS 2470
>NP_149015
          Length = 5142

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1    LETTSILRS 9
            LETT++LRS
Sbjct: 2462 LETTAVLRS 2470
>XP_006715989
          Length = 5148

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1    LETTSILRS 9
            LETT++LRS
Sbjct: 2462 LETTAVLRS 2470
>NP_001245210
          Length = 868

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET SILRS
Sbjct: 664 LETASILRS 672
>NP_000242
          Length = 934

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET SILRS
Sbjct: 730 LETASILRS 738
>XP_005264389
          Length = 921

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET SILRS
Sbjct: 730 LETASILRS 738
>NP_001243000
          Length = 5207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LETTSILRS 9
            L+TT +LRS
Sbjct: 3530 LDTTRLLRS 3538
>XP_005257603
          Length = 5256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LETTSILRS 9
            L+TT +LRS
Sbjct: 3579 LDTTRLLRS 3587
>XP_006722058
          Length = 5256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LETTSILRS 9
            L+TT +LRS
Sbjct: 3579 LDTTRLLRS 3587
>XP_005257602
          Length = 5256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LETTSILRS 9
            L+TT +LRS
Sbjct: 3579 LDTTRLLRS 3587
>NP_001159923
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ETTSILRS 9
           ETTS LRS
Sbjct: 363 ETTSHLRS 370
>NP_000884
          Length = 427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ETTSILRS 9
           ETTS LRS
Sbjct: 399 ETTSHLRS 406
>NP_001161846
          Length = 1063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LETTSILR 8
          LETT I+R
Sbjct: 31 LETTRIMR 38
>NP_001161849
          Length = 1011

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LETTSILR 8
          LETT I+R
Sbjct: 40 LETTRIMR 47
>NP_055624
          Length = 1072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LETTSILR 8
          LETT I+R
Sbjct: 40 LETTRIMR 47
>NP_001258468
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 523 LETTHLLR 530
>NP_061897
          Length = 2071

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TTSILRS 9
           TTS+LRS
Sbjct: 710 TTSLLRS 716
>NP_001161847
          Length = 1072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LETTSILR 8
          LETT I+R
Sbjct: 40 LETTRIMR 47
>XP_006723717
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 300 LETTHLLR 307
>XP_006723715
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 523 LETTHLLR 530
>XP_006723712
          Length = 604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 551 LETTHLLR 558
>XP_006723711
          Length = 670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 617 LETTHLLR 624
>XP_006723710
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 636 LETTHLLR 643
>XP_006723709
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT +LR
Sbjct: 636 LETTHLLR 643
>XP_006715350
          Length = 1040

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT I+R
Sbjct: 102 LETTRIMR 109
>XP_006715349
          Length = 1101

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT I+R
Sbjct: 102 LETTRIMR 109
>XP_005249561
          Length = 1134

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           LETT I+R
Sbjct: 102 LETTRIMR 109
>XP_005264436
          Length = 1881

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TTSILRS 9
           TTS+LRS
Sbjct: 710 TTSLLRS 716
>XP_006712098
          Length = 2059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TTSILRS 9
           TTS+LRS
Sbjct: 698 TTSLLRS 704
>XP_006712097
          Length = 2070

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TTSILRS 9
           TTS+LRS
Sbjct: 710 TTSLLRS 716
>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LETTSILRS 9
            L+TT +LR+
Sbjct: 4290 LKTTEVLRN 4298
>NP_001136272
          Length = 3144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TTSILRS 9
            TTS++RS
Sbjct: 1424 TTSVIRS 1430
>NP_001008212
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 458 LETMTILRA 466
>NP_068815
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 458 LETMTILRA 466
>NP_001008214
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 458 LETMTILRA 466
>NP_001008213
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 458 LETMTILRA 466
>NP_001180211
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           L TT+ILR
Sbjct: 297 LNTTAILR 304
>XP_005252395
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 401 LETMTILRA 409
>XP_005252394
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 452 LETMTILRA 460
>XP_005252393
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LETTSILRS 9
           LET +ILR+
Sbjct: 452 LETMTILRA 460
>XP_006713948
          Length = 747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTSILR 8
           L TT+ILR
Sbjct: 298 LNTTAILR 305
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.121    0.272 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 336,273
Number of extensions: 467
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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