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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     299
NP_671714                                                              20     299
XP_006715890                                                           20     299
XP_005250160                                                           20     299
XP_005250159                                                           20     299
NP_004774                                                              18    1935
NP_001230314                                                           16    5631
NP_001003828                                                           16    5631
NP_543016                                                              16    5631
NP_001230315                                                           16    5631
NP_037459                                                              16    5631
XP_005261653                                                           16    5631
XP_006724299                                                           16    5631
XP_005261652                                                           16    5631
XP_005261651                                                           16    5631
XP_005261650                                                           16    5631
XP_005268245                                                           16    5631
NP_150374                                                              16    5631
NP_065842                                                              15    9605
NP_079418                                                              15    9605
NP_005723                                                              15    9605
NP_001258349                                                           15    9605
NP_060245                                                              15    9605
NP_001137359                                                           15    9605
NP_004227                                                              15    9605
NP_079148                                                              15    9605
XP_005266042                                                           15    9605
XP_005266041                                                           15    9605

>NP_005742
          Length = 3907

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LETTLEAQH 9
            LETTL+AQH
Sbjct: 3175 LETTLKAQH 3183



>NP_671714
          Length = 3899

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LETTLEAQH 9
            LETTL+AQH
Sbjct: 3167 LETTLKAQH 3175



>XP_006715890
          Length = 3860

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LETTLEAQH 9
            LETTL+AQH
Sbjct: 3128 LETTLKAQH 3136



>XP_005250160
          Length = 3939

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LETTLEAQH 9
            LETTL+AQH
Sbjct: 3207 LETTLKAQH 3215



>XP_005250159
          Length = 3952

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LETTLEAQH 9
            LETTL+AQH
Sbjct: 3220 LETTLKAQH 3228



>NP_004774
          Length = 1049

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LETTLEAQH 9
           LETT +AQH
Sbjct: 773 LETTPKAQH 781



>NP_001230314
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 112 LQTVLEAVH 120



>NP_001003828
          Length = 397

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 182 LQTVLEAVH 190



>NP_543016
          Length = 464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TTLEAQH 9
           TTL AQH
Sbjct: 378 TTLAAQH 384



>NP_001230315
          Length = 289

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 97  LQTVLEAVH 105



>NP_037459
          Length = 364

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 149 LQTVLEAVH 157



>XP_005261653
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 97  LQTVLEAVH 105



>XP_006724299
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 112 LQTVLEAVH 120



>XP_005261652
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 112 LQTVLEAVH 120



>XP_005261651
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 112 LQTVLEAVH 120



>XP_005261650
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LETTLEAQH 9
           L+T LEA H
Sbjct: 112 LQTVLEAVH 120



>XP_005268245
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LETTLEAQH 9
           LET+LEA++
Sbjct: 232 LETSLEAKN 240



>NP_150374
          Length = 485

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LETTLEAQH 9
           LET+LEA++
Sbjct: 179 LETSLEAKN 187



>NP_065842
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LETTLEAQH 9
           LETT +++H
Sbjct: 745 LETTPKSEH 753



>NP_079418
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LETTLEAQH 9
           LETT +++H
Sbjct: 597 LETTPKSEH 605



>NP_005723
          Length = 1312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LETTLE 6
           LETTLE
Sbjct: 915 LETTLE 920



>NP_001258349
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTLEAQ 8
           L TTL+AQ
Sbjct: 178 LATTLDAQ 185



>NP_060245
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LETTLEAQ 8
           L TTL+AQ
Sbjct: 285 LATTLDAQ 292



>NP_001137359
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ETTLEA 7
           ETTLEA
Sbjct: 140 ETTLEA 145



>NP_004227
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ETTLEA 7
           ETTLEA
Sbjct: 133 ETTLEA 138



>NP_079148
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ETTLEAQH 9
           ++TLEAQ+
Sbjct: 446 DSTLEAQY 453



>XP_005266042
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ETTLEAQH 9
           ++TLEAQ+
Sbjct: 390 DSTLEAQY 397



>XP_005266041
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ETTLEAQH 9
           ++TLEAQ+
Sbjct: 390 DSTLEAQY 397



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.116    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 430,762
Number of extensions: 742
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)