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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000242                                                              18    1135
XP_005264389                                                           18    1135
NP_443095                                                              17    3301
NP_005075                                                              15    9605
NP_001161829                                                           15    9605
NP_001161842                                                           15    9605
NP_055595                                                              15    9605
NP_055429                                                              15    9605
XP_006715348                                                           15    9605
XP_005249560                                                           15    9605
XP_005249466                                                           15    9605
XP_005249465                                                           15    9605
NP_878248                                                              15    9605

>NP_000242
          Length = 934

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  LESAAEVG 8
          LESAAEVG
Sbjct: 11 LESAAEVG 18



>XP_005264389
          Length = 921

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  LESAAEVG 8
          LESAAEVG
Sbjct: 11 LESAAEVG 18



>NP_443095
          Length = 104

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LESAAEVG 8
          LESAAE G
Sbjct: 36 LESAAEAG 43



>NP_005075
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ESAAEVG 8
           +SAAE+G
Sbjct: 193 QSAAEIG 199



>NP_001161829
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ESAAEVG 8
           +SAAE+G
Sbjct: 193 QSAAEIG 199



>NP_001161842
          Length = 1782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ESAAEVG 8
           ESA EVG
Sbjct: 146 ESAGEVG 152



>NP_055595
          Length = 1698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ESAAEVG 8
           ESA EVG
Sbjct: 94  ESAGEVG 100



>NP_055429
          Length = 1843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LESAAEVGL 9
           LES  E+GL
Sbjct: 578 LESLVELGL 586



>XP_006715348
          Length = 1734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ESAAEVG 8
           ESA EVG
Sbjct: 94  ESAGEVG 100



>XP_005249560
          Length = 1750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ESAAEVG 8
           ESA EVG
Sbjct: 146 ESAGEVG 152



>XP_005249466
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ESAAEVG 8
           +SAAE+G
Sbjct: 193 QSAAEIG 199



>XP_005249465
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ESAAEVG 8
           +SAAE+G
Sbjct: 193 QSAAEIG 199



>NP_878248
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ESAAEVG 8
           ESAAE G
Sbjct: 132 ESAAETG 138



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.123    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 277,228
Number of extensions: 330
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)