Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     390
NP_004561                                                              20     390
NP_001275703                                                           20     390
XP_005253450                                                           20     390
XP_006719151                                                           20     390
NP_004661                                                              17    2528
NP_001015880                                                           17    2528
NP_005872                                                              17    4312
NP_001258855                                                           17    4312
NP_001116429                                                           17    4312
NP_005434                                                              17    4312
NP_000128                                                              16    7355
NP_001077083                                                           16    7355
NP_149975                                                              16    7355
NP_001269271                                                           16    7355
NP_001253970                                                           16    7355
NP_112224                                                              15    9605
NP_001265327                                                           15    9605
NP_001275582                                                           15    9605
NP_002903                                                              15    9605
NP_001273361                                                           15    9605
NP_001273360                                                           15    9605
NP_004850                                                              15    9605
XP_005257069                                                           15    9605
XP_005253212                                                           15    9605
XP_005253211                                                           15    9605
XP_005267148                                                           15    9605
XP_005267147                                                           15    9605
XP_006715607                                                           15    9605
XP_006715606                                                           15    9605

>NP_001275701
          Length = 1486

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEMMLYAG 8
            LEMMLYAG
Sbjct: 1158 LEMMLYAG 1165



>NP_004561
          Length = 1445

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEMMLYAG 8
            LEMMLYAG
Sbjct: 1117 LEMMLYAG 1124



>NP_001275703
          Length = 1264

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LEMMLYAG 8
           LEMMLYAG
Sbjct: 936 LEMMLYAG 943



>XP_005253450
          Length = 1486

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEMMLYAG 8
            LEMMLYAG
Sbjct: 1158 LEMMLYAG 1165



>XP_006719151
          Length = 1486

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LEMMLYAG 8
            LEMMLYAG
Sbjct: 1158 LEMMLYAG 1165



>NP_004661
          Length = 614

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MLYAGP 9
           MLYAGP
Sbjct: 485 MLYAGP 490



>NP_001015880
          Length = 619

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MLYAGP 9
           MLYAGP
Sbjct: 490 MLYAGP 495



>NP_005872
          Length = 412

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MMLYAG 8
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001258855
          Length = 335

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MMLYAG 8
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001116429
          Length = 365

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  MMLYAG 8
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_005434
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MLYAGP 9
           M+YAGP
Sbjct: 495 MMYAGP 500



>NP_000128
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   LEMMLYAGP 9
           LEM  + GP
Sbjct: 200 LEMAFFVGP 208



>NP_001077083
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EMMLYAGP 9
           EMML  GP
Sbjct: 503 EMMLALGP 510



>NP_149975
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EMMLYAGP 9
           +MM Y GP
Sbjct: 157 DMMSYIGP 164



>NP_001269271
          Length = 600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EMMLYAGP 9
           E +LY GP
Sbjct: 98  EELLYTGP 105



>NP_001253970
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EMMLYAGP 9
           E +LY GP
Sbjct: 140 EELLYTGP 147



>NP_112224
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LEMMLYAGP 9
            +EM++ AGP
Sbjct: 1794 VEMVIPAGP 1802



>NP_001265327
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LEMMLYAGP 9
           LE+++ AGP
Sbjct: 415 LELVVSAGP 423



>NP_001275582
          Length = 1679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMMLYAGP 9
            ++ML AGP
Sbjct: 1642 QLMLTAGP 1649



>NP_002903
          Length = 3130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1146 EAMLFKGP 1153



>NP_001273361
          Length = 3052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1068 EAMLFKGP 1075



>NP_001273360
          Length = 3052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1068 EAMLFKGP 1075



>NP_004850
          Length = 1675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMMLYAGP 9
            ++ML AGP
Sbjct: 1638 QLMLTAGP 1645



>XP_005257069
          Length = 1682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EMMLYAGP 9
            ++ML AGP
Sbjct: 1645 QLMLTAGP 1652



>XP_005253212
          Length = 1838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LEMMLYAGP 9
            +EM++ AGP
Sbjct: 1783 VEMVIPAGP 1791



>XP_005253211
          Length = 1881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LEMMLYAGP 9
            +EM++ AGP
Sbjct: 1826 VEMVIPAGP 1834



>XP_005267148
          Length = 2679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1146 EAMLFKGP 1153



>XP_005267147
          Length = 2689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1146 EAMLFKGP 1153



>XP_006715607
          Length = 3052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1068 EAMLFKGP 1075



>XP_006715606
          Length = 3130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    EMMLYAGP 9
            E ML+ GP
Sbjct: 1146 EAMLFKGP 1153



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.144    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 559,436
Number of extensions: 782
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)