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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     869
NP_671714                                                              19     869
XP_006715890                                                           19     869
XP_005250160                                                           19     869
XP_005250159                                                           19     869
NP_006036                                                              16    7355
NP_060440                                                              16    7355
NP_115820                                                              15    9605
NP_000217                                                              15    9605
NP_002266                                                              15    9605
NP_942147                                                              15    9605
XP_005259228                                                           15    9605
XP_005259227                                                           15    9605
XP_005259226                                                           15    9605
XP_005257402                                                           15    9605
XP_006721954                                                           15    9605
XP_006721506                                                           15    9605
XP_005256500                                                           15    9605
XP_006721505                                                           15    9605
XP_006721504                                                           15    9605
NP_998762                                                              15    9605

>NP_005742
          Length = 3907

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LEMEVELSS 9
            LEM+VELSS
Sbjct: 3134 LEMQVELSS 3142



>NP_671714
          Length = 3899

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LEMEVELSS 9
            LEM+VELSS
Sbjct: 3126 LEMQVELSS 3134



>XP_006715890
          Length = 3860

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LEMEVELSS 9
            LEM+VELSS
Sbjct: 3087 LEMQVELSS 3095



>XP_005250160
          Length = 3939

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LEMEVELSS 9
            LEM+VELSS
Sbjct: 3166 LEMQVELSS 3174



>XP_005250159
          Length = 3952

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LEMEVELSS 9
            LEM+VELSS
Sbjct: 3179 LEMQVELSS 3187



>NP_006036
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LEMEVEL 7
           LEME+EL
Sbjct: 639 LEMEMEL 645



>NP_060440
          Length = 1164

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EMEVELS 8
           E+EVELS
Sbjct: 829 ELEVELS 835



>NP_115820
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LEMEVELSS 9
           L  EVELSS
Sbjct: 491 LSEEVELSS 499



>NP_000217
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EMEVELSS 9
           E+E+EL S
Sbjct: 379 ELEIELQS 386



>NP_002266
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EMEVELSS 9
           E+E+EL S
Sbjct: 331 ELEIELQS 338



>NP_942147
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEMEVELS 8
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005259228
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEMEVELS 8
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005259227
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEMEVELS 8
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005259226
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LEMEVELS 8
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005257402
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EMEVELSS 9
           E+E+EL S
Sbjct: 331 ELEIELQS 338



>XP_006721954
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EMEVELSS 9
           E+E+EL S
Sbjct: 331 ELEIELQS 338



>XP_006721506
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EMEVELSS 9
          EME+E+S+
Sbjct: 18 EMEMEISN 25



>XP_005256500
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EMEVELSS 9
          EME+E+S+
Sbjct: 66 EMEMEISN 73



>XP_006721505
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EMEVELSS 9
           EME+E+S+
Sbjct: 100 EMEMEISN 107



>XP_006721504
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EMEVELSS 9
           EME+E+S+
Sbjct: 100 EMEMEISN 107



>NP_998762
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EMEVELSS 9
           EME+E+S+
Sbjct: 100 EMEMEISN 107



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294    0.115    0.243 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,821
Number of extensions: 572
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)