Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     390
NP_001035957                                                           20     390
XP_006721991                                                           20     390
XP_006721990                                                           20     390
XP_006721989                                                           20     390
XP_005258041                                                           20     390
XP_006721988                                                           20     390
XP_006721987                                                           20     390
XP_006721986                                                           20     390
XP_005258040                                                           20     390
XP_006721985                                                           20     390
NP_056377                                                              17    3301
NP_001241657                                                           16    7355
NP_001241658                                                           16    7355
NP_009008                                                              16    7355
NP_001241656                                                           16    7355
NP_001241655                                                           16    7355
NP_001121598                                                           16    7355
XP_005267350                                                           16    7355
NP_112492                                                              15    9605
>NP_000258
          Length = 2818

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 687 LEVALYMFL 695
>NP_001035957
          Length = 2839

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 687 LEVALYMFL 695
>XP_006721991
          Length = 1632

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 697 LEVALYMFL 705
>XP_006721990
          Length = 2823

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 697 LEVALYMFL 705
>XP_006721989
          Length = 2823

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 697 LEVALYMFL 705
>XP_005258041
          Length = 2836

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 687 LEVALYMFL 695
>XP_006721988
          Length = 2846

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 697 LEVALYMFL 705
>XP_006721987
          Length = 2849

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 697 LEVALYMFL 705
>XP_006721986
          Length = 2854

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 684 LEVALYMFL 692
>XP_005258040
          Length = 2857

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 687 LEVALYMFL 695
>XP_006721985
          Length = 2867

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LEMALYMFL 9
           LE+ALYMFL
Sbjct: 697 LEVALYMFL 705
>NP_056377
          Length = 489

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  LEMALYMFL 9
          LEMA+ MFL
Sbjct: 42 LEMAVTMFL 50
>NP_001241657
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001241658
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_009008
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001241656
          Length = 149

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001241655
          Length = 135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001121598
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>XP_005267350
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EMALYMFL 9
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_112492
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LEMALYM 7
           LE+ALY+
Sbjct: 456 LELALYL 462
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.351    0.154    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,982
Number of extensions: 382
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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