Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_000426                                                              16    5631
NP_848652                                                              16    5631
XP_005259981                                                           16    5631
XP_006710219                                                           16    5631
NP_000778                                                              16    7355
NP_056046                                                              16    7355
NP_060474                                                              16    7355
XP_005247626                                                           16    7355
XP_005247625                                                           16    7355
NP_001005374                                                           15    9605
NP_060420                                                              15    9605
NP_612370                                                              15    9605
NP_001005373                                                           15    9605
NP_003909                                                              15    9605
NP_001171632                                                           15    9605
NP_055950                                                              15    9605
NP_001230271                                                           15    9605
XP_006724585                                                           15    9605
XP_006724584                                                           15    9605
XP_005274676                                                           15    9605
XP_005274674                                                           15    9605
XP_005274675                                                           15    9605
XP_005257548                                                           15    9605
XP_006717379                                                           15    9605
XP_005250292                                                           15    9605
NP_001005476                                                           15    9605
NP_003619                                                              15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LELMRSSVD 9
            LELM+SSVD
Sbjct: 1580 LELMQSSVD 1588
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LELMRSSVD 9
            LELM+SSVD
Sbjct: 1580 LELMQSSVD 1588
>NP_000426
          Length = 2321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELMRSSVD 9
            EL+RSS D
Sbjct: 1523 ELLRSSAD 1530
>NP_848652
          Length = 302

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LELMRSSVD 9
           +EL+R SVD
Sbjct: 208 VELLRMSVD 216
>XP_005259981
          Length = 2269

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELMRSSVD 9
            EL+RSS D
Sbjct: 1471 ELLRSSAD 1478
>XP_006710219
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LELMRSSVD 9
           +EL+R SVD
Sbjct: 124 VELLRMSVD 132
>NP_000778
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LELMRSSVD 9
           LEL +S+VD
Sbjct: 500 LELCKSAVD 508
>NP_056046
          Length = 1436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           LELM  S+D
Sbjct: 112 LELMEESLD 120
>NP_060474
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LELMRSSV 8
           LEL+RSS+
Sbjct: 179 LELLRSSL 186
>XP_005247626
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LELMRSSV 8
           LEL+RSS+
Sbjct: 195 LELLRSSL 202
>XP_005247625
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LELMRSSV 8
           LEL+RSS+
Sbjct: 195 LELLRSSL 202
>NP_001005374
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           LEL R S+D
Sbjct: 488 LELKRKSLD 496
>NP_060420
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MRSSVD 9
           MRSSVD
Sbjct: 920 MRSSVD 925
>NP_612370
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           LEL R S+D
Sbjct: 488 LELKRKSLD 496
>NP_001005373
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           LEL R S+D
Sbjct: 488 LELKRKSLD 496
>NP_003909
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 14 LELLRSS 20
>NP_001171632
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 14 LELLRSS 20
>NP_055950
          Length = 2012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LELMRSSV 8
            LEL+RS V
Sbjct: 1571 LELLRSGV 1578
>NP_001230271
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MRSSVD 9
           MRSSVD
Sbjct: 920 MRSSVD 925
>XP_006724585
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 12 LELLRSS 18
>XP_006724584
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 14 LELLRSS 20
>XP_005274676
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 14 LELLRSS 20
>XP_005274674
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 14 LELLRSS 20
>XP_005274675
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LELMRSS 7
          LEL+RSS
Sbjct: 14 LELLRSS 20
>XP_005257548
          Length = 749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MRSSVD 9
           MRSSVD
Sbjct: 527 MRSSVD 532
>XP_006717379
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           LEL R S+D
Sbjct: 488 LELKRKSLD 496
>XP_005250292
          Length = 1654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LELMRSSV 8
            LEL+RS V
Sbjct: 1213 LELLRSGV 1220
>NP_001005476
          Length = 1149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           L L+R S+D
Sbjct: 620 LRLLRKSID 628
>NP_003619
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LELMRSSVD 9
           L L+R S+D
Sbjct: 620 LRLLRKSID 628
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.129    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,499
Number of extensions: 461
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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