Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001138390                                                           18    1482
NP_001138386                                                           18    1482
NP_001138387                                                           18    1482
NP_000132                                                              18    1482
NP_075259                                                              18    1482
NP_001138388                                                           18    1482
NP_001138391                                                           18    1482
NP_001138385                                                           18    1482
NP_075418                                                              18    1482
XP_006717776                                                           18    1482
XP_006717775                                                           18    1482
XP_006717774                                                           18    1482
XP_006717773                                                           18    1482
XP_006717772                                                           18    1482
XP_006717771                                                           18    1482
NP_061848                                                              16    7355
NP_787125                                                              16    7355
NP_997304                                                              16    7355
NP_004106                                                              16    7355
NP_001012680                                                           15    9605
NP_001012682                                                           15    9605
NP_002385                                                              15    9605
NP_001013269                                                           15    9605
NP_006112                                                              15    9605
>NP_001138390
          Length = 704

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 261 LEIAIYCIG 269
>NP_001138386
          Length = 709

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 264 LEIAIYCIG 272
>NP_001138387
          Length = 707

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 287 LEIAIYCIG 295
>NP_000132
          Length = 821

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 376 LEIAIYCIG 384
>NP_075259
          Length = 822

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 377 LEIAIYCIG 385
>NP_001138388
          Length = 706

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 261 LEIAIYCIG 269
>NP_001138391
          Length = 680

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 288 LEIAIYCIG 296
>NP_001138385
          Length = 769

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 377 LEIAIYCIG 385
>NP_075418
          Length = 732

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 287 LEIAIYCIG 295
>XP_006717776
          Length = 806

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 395 LEIAIYCIG 403
>XP_006717775
          Length = 726

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 281 LEIAIYCIG 289
>XP_006717774
          Length = 752

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 307 LEIAIYCIG 315
>XP_006717773
          Length = 807

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 396 LEIAIYCIG 404
>XP_006717772
          Length = 838

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 395 LEIAIYCIG 403
>XP_006717771
          Length = 839

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LEIAIYRIG 9
           LEIAIY IG
Sbjct: 396 LEIAIYCIG 404
>NP_061848
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LEIAIYRIG 9
           LE+  YR+G
Sbjct: 94  LELEAYRLG 102
>NP_787125
          Length = 252

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LEIAIYR 7
           LE+A+YR
Sbjct: 206 LEVAMYR 212
>NP_997304
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LEIAIYRIG 9
           LE+ IYR G
Sbjct: 126 LEVDIYRNG 134
>NP_004106
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LEIAIYR 7
           LE+A+YR
Sbjct: 201 LEVAMYR 207
>NP_001012680
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AIYRIG 9
           A+YRIG
Sbjct: 224 ALYRIG 229
>NP_001012682
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AIYRIG 9
           A+YRIG
Sbjct: 161 ALYRIG 166
>NP_002385
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AIYRIG 9
           A+YRIG
Sbjct: 223 ALYRIG 228
>NP_001013269
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AIYRIG 9
           A+YRIG
Sbjct: 122 ALYRIG 127
>NP_006112
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LEIAIYR 7
           LEIA YR
Sbjct: 477 LEIATYR 483
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.161    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 373,420
Number of extensions: 436
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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