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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005259175                                                           17    4312
XP_005259174                                                           17    4312
XP_006723374                                                           17    4312
XP_005259173                                                           17    4312
XP_006723373                                                           17    4312
XP_006723372                                                           17    4312
XP_005259172                                                           17    4312
XP_005259171                                                           17    4312
XP_006723371                                                           17    4312
NP_067008                                                              17    4312
NP_066997                                                              16    5631
NP_116250                                                              16    7355
NP_054878                                                              16    7355
NP_003276                                                              16    7355
XP_006715649                                                           16    7355
XP_006715648                                                           16    7355
XP_005265151                                                           16    7355
XP_006713183                                                           16    7355
XP_006713182                                                           16    7355
NP_001704                                                              15    9605
NP_001020029                                                           15    9605
NP_000338                                                              15    9605
NP_892018                                                              15    9605
NP_002806                                                              15    9605
NP_001257411                                                           15    9605
NP_705833                                                              15    9605
XP_005259608                                                           15    9605
XP_006720304                                                           15    9605
XP_005268080                                                           15    9605
XP_005247174                                                           15    9605

>XP_005259175
          Length = 595

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 243 IEASVKQEV 251



>XP_005259174
          Length = 799

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 447 IEASVKQEV 455



>XP_006723374
          Length = 1159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 807 IEASVKQEV 815



>XP_005259173
          Length = 1099

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 747 IEASVKQEV 755



>XP_006723373
          Length = 1120

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 768 IEASVKQEV 776



>XP_006723372
          Length = 1159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 807 IEASVKQEV 815



>XP_005259172
          Length = 1159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 807 IEASVKQEV 815



>XP_005259171
          Length = 1159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 807 IEASVKQEV 815



>XP_006723371
          Length = 1159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 807 IEASVKQEV 815



>NP_067008
          Length = 1159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           +EA+ KQEV
Sbjct: 807 IEASVKQEV 815



>NP_066997
          Length = 923

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EATTKQE 8
           EATT+QE
Sbjct: 486 EATTQQE 492



>NP_116250
          Length = 654

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           LEA+TK E+
Sbjct: 509 LEASTKPEM 517



>NP_054878
          Length = 2564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LEATTKQE 8
           LEA TKQ+
Sbjct: 250 LEADTKQD 257



>NP_003276
          Length = 1358

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LEATTKQEV 9
          LEA+ +QEV
Sbjct: 83 LEASAEQEV 91



>XP_006715649
          Length = 584

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           LEA+TK E+
Sbjct: 439 LEASTKPEM 447



>XP_006715648
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEATTKQEV 9
           LEA+TK E+
Sbjct: 471 LEASTKPEM 479



>XP_005265151
          Length = 2058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LEATTKQE 8
           LEA TKQ+
Sbjct: 250 LEADTKQD 257



>XP_006713183
          Length = 2258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LEATTKQE 8
           LEA TKQ+
Sbjct: 250 LEADTKQD 257



>XP_006713182
          Length = 2422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LEATTKQE 8
           LEA TKQ+
Sbjct: 250 LEADTKQD 257



>NP_001704
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EATTKQEV 9
           EATT+Q++
Sbjct: 387 EATTEQQL 394



>NP_001020029
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LEATTKQE 8
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371



>NP_000338
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LEATTKQE 8
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371



>NP_892018
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LEATTK 6
           LEATTK
Sbjct: 432 LEATTK 437



>NP_002806
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LEATTKQEV 9
           +EA T QEV
Sbjct: 99  MEAATGQEV 107



>NP_001257411
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LEATTKQEV 9
           +EA T QEV
Sbjct: 99  MEAATGQEV 107



>NP_705833
          Length = 2970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    EATTKQEV 9
            E+T KQEV
Sbjct: 2304 ESTYKQEV 2311



>XP_005259608
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LEATTK 6
           LEATTK
Sbjct: 466 LEATTK 471



>XP_006720304
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LEATTKQE 8
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371



>XP_005268080
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LEATTKQE 8
            L+ATTK++
Sbjct: 1364 LQATTKEK 1371



>XP_005247174
          Length = 2679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    EATTKQEV 9
            E+T KQEV
Sbjct: 2013 ESTYKQEV 2020



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.107    0.240 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 414,371
Number of extensions: 515
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)