Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     869
NP_671714                                                              19     869
XP_006715890                                                           19     869
XP_005250160                                                           19     869
XP_005250159                                                           19     869
NP_002105                                                              17    4312
XP_005249099                                                           17    4312
NP_057274                                                              16    5631
XP_006711409                                                           16    5631
NP_001096080                                                           16    7355
NP_443135                                                              16    7355
NP_064578                                                              16    7355
XP_006725237                                                           16    7355
XP_005272597                                                           16    7355
XP_006718701                                                           16    7355
XP_005274169                                                           16    7355
XP_005274166                                                           16    7355
XP_005274165                                                           16    7355
XP_005274163                                                           16    7355
NP_001166951                                                           15    9605
NP_001166950                                                           15    9605
NP_057297                                                              15    9605
NP_056000                                                              15    9605
NP_001159743                                                           15    9605
NP_001273721                                                           15    9605
NP_001010888                                                           15    9605
NP_001273723                                                           15    9605
NP_001273724                                                           15    9605
NP_113584                                                              15    9605
NP_001273722                                                           15    9605
NP_694568                                                              15    9605
NP_001036111                                                           15    9605
NP_001070863                                                           15    9605
NP_001070862                                                           15    9605
NP_005376                                                              15    9605
NP_057042                                                              15    9605
NP_057417                                                              15    9605
NP_848620                                                              15    9605
XP_005262317                                                           15    9605
XP_005262309                                                           15    9605
XP_005262308                                                           15    9605
XP_006724700                                                           15    9605
XP_005262307                                                           15    9605
XP_006724699                                                           15    9605
XP_005262306                                                           15    9605
XP_005262029                                                           15    9605
XP_005262028                                                           15    9605
XP_005262026                                                           15    9605
XP_006724637                                                           15    9605
XP_005262025                                                           15    9605
XP_005262024                                                           15    9605
XP_005262023                                                           15    9605
XP_005262022                                                           15    9605
XP_005255284                                                           15    9605
XP_005255283                                                           15    9605
XP_006720936                                                           15    9605
XP_006720935                                                           15    9605
XP_005255282                                                           15    9605
XP_005254846                                                           15    9605
XP_005265233                                                           15    9605
XP_005265231                                                           15    9605
XP_006713237                                                           15    9605
XP_006713236                                                           15    9605
XP_006713235                                                           15    9605
XP_006713234                                                           15    9605
XP_005265230                                                           15    9605
XP_005265016                                                           15    9605
XP_005265015                                                           15    9605
XP_005265014                                                           15    9605
XP_005265013                                                           15    9605
XP_006710746                                                           15    9605
XP_005270998                                                           15    9605
XP_005270996                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDRSSESQS 9
            LDRSS+SQS
Sbjct: 1757 LDRSSKSQS 1765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LDRSSESQ 8
           LDR +ESQ
Sbjct: 611 LDRMAESQ 618
>NP_671714
          Length = 3899

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDRSSESQS 9
            LDRSS+SQS
Sbjct: 1757 LDRSSKSQS 1765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LDRSSESQ 8
           LDR +ESQ
Sbjct: 611 LDRMAESQ 618
>XP_006715890
          Length = 3860

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDRSSESQS 9
            LDRSS+SQS
Sbjct: 1710 LDRSSKSQS 1718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LDRSSESQ 8
           LDR +ESQ
Sbjct: 611 LDRMAESQ 618
>XP_005250160
          Length = 3939

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDRSSESQS 9
            LDRSS+SQS
Sbjct: 1789 LDRSSKSQS 1797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LDRSSESQ 8
           LDR +ESQ
Sbjct: 611 LDRMAESQ 618
>XP_005250159
          Length = 3952

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDRSSESQS 9
            LDRSS+SQS
Sbjct: 1789 LDRSSKSQS 1797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LDRSSESQ 8
           LDR +ESQ
Sbjct: 611 LDRMAESQ 618
>NP_002105
          Length = 2718

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   DRSSESQS 9
           DRS+ESQ+
Sbjct: 549 DRSAESQA 556
>XP_005249099
          Length = 2026

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   DRSSESQS 9
           DRS+ESQ+
Sbjct: 549 DRSAESQA 556
>NP_057274
          Length = 495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           DR +ESQS
Sbjct: 168 DRQAESQS 175
>XP_006711409
          Length = 495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           DR +ESQS
Sbjct: 168 DRQAESQS 175
>NP_001096080
          Length = 1024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDRSSES 7
           +DRSSES
Sbjct: 671 VDRSSES 677
>NP_443135
          Length = 1024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDRSSES 7
           +DRSSES
Sbjct: 671 VDRSSES 677
>NP_064578
          Length = 1322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           D SSESQS
Sbjct: 764 DVSSESQS 771
>XP_006725237
          Length = 878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDRSSES 7
           +DRSSES
Sbjct: 525 VDRSSES 531
>XP_005272597
          Length = 1025

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDRSSES 7
           +DRSSES
Sbjct: 672 VDRSSES 678
>XP_006718701
          Length = 1309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           D SSESQS
Sbjct: 765 DVSSESQS 772
>XP_005274169
          Length = 1272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           D SSESQS
Sbjct: 728 DVSSESQS 735
>XP_005274166
          Length = 1323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           D SSESQS
Sbjct: 779 DVSSESQS 786
>XP_005274165
          Length = 1323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           D SSESQS
Sbjct: 765 DVSSESQS 772
>XP_005274163
          Length = 1337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           D SSESQS
Sbjct: 779 DVSSESQS 786
>NP_001166951
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 211 LDRSSE 216
>NP_001166950
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 260 LDRSSE 265
>NP_057297
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 368 LDRSSE 373
>NP_056000
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 313 LDRSSE 318
>NP_001159743
          Length = 1277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LDRSSE 6
            LDRSSE
Sbjct: 1075 LDRSSE 1080
>NP_001273721
          Length = 1339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LDRSSE 6
            LDRSSE
Sbjct: 1137 LDRSSE 1142
>NP_001010888
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  LDRSSESQS 9
          LDR S SQS
Sbjct: 86 LDRPSFSQS 94
>NP_001273723
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 394 LDRSSE 399
>NP_001273724
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 372 LDRSSE 377
>NP_113584
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3230 LQRSSESE 3237
>NP_001273722
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 310 LDRSSE 315
>NP_694568
          Length = 652

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 450 LDRSSE 455
>NP_001036111
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSSESQS 9
           RSSESQ+
Sbjct: 828 RSSESQT 834
>NP_001070863
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           + SSESQS
Sbjct: 273 ENSSESQS 280
>NP_001070862
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           + SSESQS
Sbjct: 274 ENSSESQS 281
>NP_005376
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 683 DRSSES 688
>NP_057042
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           + SSESQS
Sbjct: 274 ENSSESQS 281
>NP_057417
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    DRSSESQS 9
            DRS  SQS
Sbjct: 1789 DRSGSSQS 1796
>NP_848620
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RSSESQS 9
          +SSESQS
Sbjct: 7  KSSESQS 13
>XP_005262317
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  LDRSSESQS 9
          LDR S SQS
Sbjct: 86 LDRPSFSQS 94
>XP_005262309
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 292 LDRSSE 297
>XP_005262308
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 292 LDRSSE 297
>XP_006724700
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 292 LDRSSE 297
>XP_005262307
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 292 LDRSSE 297
>XP_006724699
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 318 LDRSSE 323
>XP_005262306
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 320 LDRSSE 325
>XP_005262029
          Length = 4240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3096 LQRSSESE 3103
>XP_005262028
          Length = 4341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3197 LQRSSESE 3204
>XP_005262026
          Length = 4359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3230 LQRSSESE 3237
>XP_006724637
          Length = 4365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3221 LQRSSESE 3228
>XP_005262025
          Length = 4373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3229 LQRSSESE 3236
>XP_005262024
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3230 LQRSSESE 3237
>XP_005262023
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3230 LQRSSESE 3237
>XP_005262022
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LDRSSESQ 8
            L RSSES+
Sbjct: 3230 LQRSSESE 3237
>XP_005255284
          Length = 2581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    DRSSESQS 9
            DRS  SQS
Sbjct: 1789 DRSGSSQS 1796
>XP_005255283
          Length = 2751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    DRSSESQS 9
            DRS  SQS
Sbjct: 1789 DRSGSSQS 1796
>XP_006720936
          Length = 2751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    DRSSESQS 9
            DRS  SQS
Sbjct: 1788 DRSGSSQS 1795
>XP_006720935
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    DRSSESQS 9
            DRS  SQS
Sbjct: 1789 DRSGSSQS 1796
>XP_005255282
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    DRSSESQS 9
            DRS  SQS
Sbjct: 1789 DRSGSSQS 1796
>XP_005254846
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LDRSSE 6
           LDRSSE
Sbjct: 138 LDRSSE 143
>XP_005265233
          Length = 1163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 383 DRSSES 388
>XP_005265231
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 430 DRSSES 435
>XP_006713237
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 573 DRSSES 578
>XP_006713236
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 573 DRSSES 578
>XP_006713235
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 573 DRSSES 578
>XP_006713234
          Length = 1436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 683 DRSSES 688
>XP_005265230
          Length = 1463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   DRSSES 7
           DRSSES
Sbjct: 683 DRSSES 688
>XP_005265016
          Length = 849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSSESQS 9
           RSSESQ+
Sbjct: 724 RSSESQT 730
>XP_005265015
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSSESQS 9
           RSSESQ+
Sbjct: 754 RSSESQT 760
>XP_005265014
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSSESQS 9
           RSSESQ+
Sbjct: 770 RSSESQT 776
>XP_005265013
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSSESQS 9
           RSSESQ+
Sbjct: 807 RSSESQT 813
>XP_006710746
          Length = 164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           + SSESQS
Sbjct: 153 ENSSESQS 160
>XP_005270998
          Length = 164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           + SSESQS
Sbjct: 153 ENSSESQS 160
>XP_005270996
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSSESQS 9
           + SSESQS
Sbjct: 161 ENSSESQS 168
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.279   0.0973    0.207 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,734
Number of extensions: 374
Number of successful extensions: 78
Number of sequences better than 10000.0: 73
Number of HSP's gapped: 78
Number of HSP's successfully gapped: 78
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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