Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_001091984                                                           17    2528
NP_689672                                                              17    2528
XP_005255200                                                           17    2528
NP_001278261                                                           17    3301
NP_001034553                                                           17    3301
NP_699167                                                              17    3301
XP_005269553                                                           17    3301
NP_004749                                                              17    4312
NP_077729                                                              17    4312
NP_001248764                                                           17    4312
XP_006722241                                                           17    4312
XP_006722240                                                           17    4312
XP_006722239                                                           17    4312
XP_006722238                                                           17    4312
XP_005257864                                                           17    4312
XP_006722237                                                           17    4312
XP_006722236                                                           17    4312
NP_665736                                                              16    5631
NP_005255                                                              16    5631
NP_001138925                                                           16    5631
XP_005269753                                                           16    5631
NP_062545                                                              16    5631
NP_938406                                                              16    7355
NP_938191                                                              16    7355
NP_061496                                                              16    7355
NP_938197                                                              16    7355
NP_060888                                                              15    9605
NP_001035518                                                           15    9605
NP_079170                                                              15    9605
NP_001263629                                                           15    9605
NP_612202                                                              15    9605
NP_001248321                                                           15    9605
NP_835235                                                              15    9605
XP_005267912                                                           15    9605
XP_005267911                                                           15    9605
XP_005267909                                                           15    9605
XP_005267908                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDLLLSALE 9
            LDLLL+ALE
Sbjct: 1257 LDLLLAALE 1265
>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LDLLLSALE 9
            LDLLL+ALE
Sbjct: 1257 LDLLLAALE 1265
>NP_001091984
          Length = 361

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  DLLLSALE 9
          DL+LSALE
Sbjct: 37 DLILSALE 44
>NP_689672
          Length = 402

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  DLLLSALE 9
          DL+LSALE
Sbjct: 37 DLILSALE 44
>XP_005255200
          Length = 363

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  DLLLSALE 9
          DL+LSALE
Sbjct: 75 DLILSALE 82
>NP_001278261
          Length = 841

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   DLLLSALE 9
           DLLL+ALE
Sbjct: 214 DLLLAALE 221
>NP_001034553
          Length = 1323

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   DLLLSALE 9
           DLLL+ALE
Sbjct: 696 DLLLAALE 703
>NP_699167
          Length = 348

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   DLLLSALE 9
           DLLL+ALE
Sbjct: 241 DLLLTALE 248
>XP_005269553
          Length = 358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   DLLLSALE 9
           DLLL+ALE
Sbjct: 241 DLLLTALE 248
>NP_004749
          Length = 1857

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 532 LDLLTSALD 540
>NP_077729
          Length = 1797

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 472 LDLLTSALD 480
>NP_001248764
          Length = 1848

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 532 LDLLTSALD 540
>XP_006722241
          Length = 1467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 92  LDLLTSALD 100
>XP_006722240
          Length = 1467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 92  LDLLTSALD 100
>XP_006722239
          Length = 1630

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 532 LDLLTSALD 540
>XP_006722238
          Length = 1847

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 472 LDLLTSALD 480
>XP_005257864
          Length = 1898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 532 LDLLTSALD 540
>XP_006722237
          Length = 1900

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 525 LDLLTSALD 533
>XP_006722236
          Length = 1907

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LDLLLSALE 9
           LDLL SAL+
Sbjct: 532 LDLLTSALD 540
>NP_665736
          Length = 460

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LDLLLSA 7
          LDLLLSA
Sbjct: 13 LDLLLSA 19
>NP_005255
          Length = 465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LDLLLSA 7
          LDLLLSA
Sbjct: 13 LDLLLSA 19
>NP_001138925
          Length = 460

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LDLLLSA 7
          LDLLLSA
Sbjct: 13 LDLLLSA 19
>XP_005269753
          Length = 465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LDLLLSA 7
          LDLLLSA
Sbjct: 13 LDLLLSA 19
>NP_062545
          Length = 299

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LDLLLSAL 8
          LDLLLS L
Sbjct: 44 LDLLLSCL 51
>NP_938406
          Length = 2343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    LLLSALE 9
            LLLSALE
Sbjct: 1978 LLLSALE 1984
>NP_938191
          Length = 2366

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    LLLSALE 9
            LLLSALE
Sbjct: 2001 LLLSALE 2007
>NP_061496
          Length = 2377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    LLLSALE 9
            LLLSALE
Sbjct: 2012 LLLSALE 2018
>NP_938197
          Length = 2306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    LLLSALE 9
            LLLSALE
Sbjct: 1941 LLLSALE 1947
>NP_060888
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 471 LDMLLSA 477
>NP_001035518
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 439 LDMLLSA 445
>NP_079170
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LDL+LSA
Sbjct: 284 LDLILSA 290
>NP_001263629
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  DLLLSALE 9
          +LLL+ALE
Sbjct: 20 ELLLAALE 27
>NP_612202
          Length = 892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  DLLLSALE 9
          +LLL+ALE
Sbjct: 20 ELLLAALE 27
>NP_001248321
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LDL+LSA
Sbjct: 258 LDLILSA 264
>NP_835235
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 285 LDILLSA 291
>XP_005267912
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 408 LDMLLSA 414
>XP_005267911
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 408 LDMLLSA 414
>XP_005267909
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 440 LDMLLSA 446
>XP_005267908
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LDLLLSA 7
           LD+LLSA
Sbjct: 472 LDMLLSA 478
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.145    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 342,803
Number of extensions: 448
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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