Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     869
XP_005266703                                                           19     869
NP_001269435                                                           17    3301
NP_612468                                                              17    4312
NP_057314                                                              16    7355
NP_003244                                                              16    7355
NP_002139                                                              16    7355
XP_005261096                                                           16    7355
XP_005261095                                                           16    7355
XP_005261094                                                           16    7355
NP_001138884                                                           15    9605
NP_006155                                                              15    9605
NP_001138885                                                           15    9605
NP_003346                                                              15    9605
NP_001135451                                                           15    9605
NP_005648                                                              15    9605
NP_114408                                                              15    9605
NP_001135452                                                           15    9605
NP_006697                                                              15    9605
NP_001035095                                                           15    9605
NP_085150                                                              15    9605
NP_065939                                                              15    9605
NP_001152758                                                           15    9605
NP_001185901                                                           15    9605
XP_005258921                                                           15    9605
XP_006723252                                                           15    9605
XP_006721821                                                           15    9605
XP_005254692                                                           15    9605
XP_005254691                                                           15    9605
XP_006714817                                                           15    9605
XP_005268424                                                           15    9605
XP_005268423                                                           15    9605
XP_005268422                                                           15    9605
XP_006710855                                                           15    9605
XP_006710854                                                           15    9605
NP_001073925                                                           15    9605
>NP_443179
          Length = 2170

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAVSVASPE 9
           +AVSVASPE
Sbjct: 772 VAVSVASPE 780
>XP_005266703
          Length = 1501

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAVSVASPE 9
           +AVSVASPE
Sbjct: 103 VAVSVASPE 111
>NP_001269435
          Length = 709

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LAVSVASPE 9
           L +SVASPE
Sbjct: 665 LRLSVASPE 673
>NP_612468
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LAVSVASPE 9
           LAV V SPE
Sbjct: 186 LAVKVLSPE 194
>NP_057314
          Length = 521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VSVASPE 9
           VSV+SPE
Sbjct: 373 VSVSSPE 379
>NP_003244
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AVSVASPE 9
            AVS +SPE
Sbjct: 1461 AVSASSPE 1468
>NP_002139
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VSVASPE 9
           VSV+SPE
Sbjct: 235 VSVSSPE 241
>XP_005261096
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AVSVASPE 9
            AVS +SPE
Sbjct: 1461 AVSASSPE 1468
>XP_005261095
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AVSVASPE 9
            AVS +SPE
Sbjct: 1461 AVSASSPE 1468
>XP_005261094
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AVSVASPE 9
            AVS +SPE
Sbjct: 1461 AVSASSPE 1468
>NP_001138884
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 337 SVASPE 342
>NP_006155
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 353 SVASPE 358
>NP_001138885
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 330 SVASPE 335
>NP_003346
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LAVSVASP 8
           LAV+VA P
Sbjct: 129 LAVAVAQP 136
>NP_001135451
          Length = 1975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAVSVASPE 9
            +A SVASP+
Sbjct: 1027 VAESVASPQ 1035
>NP_005648
          Length = 1972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAVSVASPE 9
            +A SVASP+
Sbjct: 1022 VAESVASPQ 1030
>NP_114408
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 637 SVASPE 642
>NP_001135452
          Length = 1977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAVSVASPE 9
            +A SVASP+
Sbjct: 1027 VAESVASPQ 1035
>NP_006697
          Length = 1098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AVSVASP 8
           AVSVA+P
Sbjct: 309 AVSVATP 315
>NP_001035095
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AVSVASP 8
           AVSVA+P
Sbjct: 309 AVSVATP 315
>NP_085150
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AVSVASP 8
          AVSVA+P
Sbjct: 20 AVSVAAP 26
>NP_065939
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 796 SVASPE 801
>NP_001152758
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 617 SVASPE 622
>NP_001185901
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 737 SVASPE 742
>XP_005258921
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SVASPE 9
          SVASPE
Sbjct: 25 SVASPE 30
>XP_006723252
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SVASPE 9
          SVASPE
Sbjct: 25 SVASPE 30
>XP_006721821
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 226 SVASPE 231
>XP_005254692
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LAVSVASPE 9
          +A SVASP+
Sbjct: 67 VAESVASPQ 75
>XP_005254691
          Length = 1972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAVSVASPE 9
            +A SVASP+
Sbjct: 1022 VAESVASPQ 1030
>XP_006714817
          Length = 827

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AVSVASP 8
           AVSVA+P
Sbjct: 309 AVSVATP 315
>XP_005268424
          Length = 1054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AVSVASP 8
           AVSVA+P
Sbjct: 309 AVSVATP 315
>XP_005268423
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AVSVASP 8
           AVSVA+P
Sbjct: 309 AVSVATP 315
>XP_005268422
          Length = 1115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AVSVASP 8
           AVSVA+P
Sbjct: 309 AVSVATP 315
>XP_006710855
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 523 SVASPE 528
>XP_006710854
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SVASPE 9
           SVASPE
Sbjct: 748 SVASPE 753
>NP_001073925
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SVASPE 9
          SVASPE
Sbjct: 25 SVASPE 30
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.113    0.269 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 609,315
Number of extensions: 1046
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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