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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000089                                                              15    9605
NP_009017                                                              15    9605
NP_001008397                                                           15    9605
XP_005267351                                                           15    9605
XP_006720075                                                           15    9605
XP_006718055                                                           15    9605
XP_006718054                                                           15    9605
XP_005270249                                                           15    9605
XP_006718053                                                           15    9605
XP_005270248                                                           15    9605
XP_005270541                                                           15    9605
NP_112189                                                              15    9605

>NP_000089
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVAMLSFQ 9
           AVA L+FQ
Sbjct: 465 AVAQLAFQ 472



>NP_009017
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AVAMLSFQ 9
           AVA+LSF+
Sbjct: 705 AVAILSFK 712



>NP_001008397
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AVAMLSFQ 9
           AVA+LSF+
Sbjct: 582 AVAILSFK 589



>XP_005267351
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AVAMLSFQ 9
           AVA+LSF+
Sbjct: 401 AVAILSFK 408



>XP_006720075
          Length = 1127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AVAMLSFQ 9
           AVA+LSF+
Sbjct: 705 AVAILSFK 712



>XP_006718055
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAVAMLSF 8
           LAVA+L+F
Sbjct: 106 LAVAVLAF 113



>XP_006718054
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAVAMLSF 8
           LAVA+L+F
Sbjct: 106 LAVAVLAF 113



>XP_005270249
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAVAMLSF 8
           LAVA+L+F
Sbjct: 106 LAVAVLAF 113



>XP_006718053
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAVAMLSF 8
           LAVA+L+F
Sbjct: 113 LAVAVLAF 120



>XP_005270248
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAVAMLSF 8
           LAVA+L+F
Sbjct: 142 LAVAVLAF 149



>XP_005270541
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVAMLSFQ 9
           AVA L+FQ
Sbjct: 465 AVAQLAFQ 472



>NP_112189
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAVAMLSF 8
           LAVA+L+F
Sbjct: 106 LAVAVLAF 113



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.136    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 211,849
Number of extensions: 158
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)