Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_808882                                                              17    4312
NP_001137544                                                           17    4312
XP_006720011                                                           17    4312
XP_006720010                                                           17    4312
NP_001005210                                                           16    5631
NP_000055                                                              16    7355
NP_078808                                                              15    9605
NP_001020607                                                           15    9605
NP_060264                                                              15    9605
NP_690043                                                              15    9605
NP_000780                                                              15    9605
NP_001171528                                                           15    9605
NP_001275915                                                           15    9605
NP_001275914                                                           15    9605
NP_009099                                                              15    9605
NP_758519                                                              15    9605
NP_000751                                                              15    9605
NP_724781                                                              15    9605
NP_940999                                                              15    9605
XP_006721800                                                           15    9605
XP_005257167                                                           15    9605
XP_005256997                                                           15    9605
XP_005256995                                                           15    9605
XP_005251551                                                           15    9605
XP_006716857                                                           15    9605
XP_006712810                                                           15    9605
XP_006712809                                                           15    9605
XP_006712808                                                           15    9605
XP_005270552                                                           15    9605
XP_005270550                                                           15    9605
XP_005270549                                                           15    9605
NP_004913                                                              15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LATNFLLKM 9
            LATNFLL+M
Sbjct: 2557 LATNFLLEM 2565
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LATNFLLKM 9
            LATNFLL+M
Sbjct: 2557 LATNFLLEM 2565
>NP_808882
          Length = 309

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LATNFLLK 8
           +ATNFLL+
Sbjct: 301 VATNFLLQ 308
>NP_001137544
          Length = 344

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LATNFLLK 8
           +ATNFLL+
Sbjct: 336 VATNFLLQ 343
>XP_006720011
          Length = 231

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LATNFLLK 8
           +ATNFLL+
Sbjct: 223 VATNFLLQ 230
>XP_006720010
          Length = 262

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LATNFLLK 8
           +ATNFLL+
Sbjct: 254 VATNFLLQ 261
>NP_001005210
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LATNFLL 7
           +ATNFLL
Sbjct: 316 VATNFLL 322
>NP_000055
          Length = 1663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LATNFLLKM 9
           L  NFLL+M
Sbjct: 471 LNVNFLLRM 479
>NP_078808
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 115 NFLIKM 120
>NP_001020607
          Length = 183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 115 NFLIKM 120
>NP_060264
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NF+LKM
Sbjct: 815 NFILKM 820
>NP_690043
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 476 NFLMKM 481
>NP_000780
          Length = 1306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    NFLLKM 9
            NFL+KM
Sbjct: 1050 NFLMKM 1055
>NP_001171528
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 476 NFLMKM 481
>NP_001275915
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LATNFLL 7
           +ATNF+L
Sbjct: 399 VATNFML 405
>NP_001275914
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LATNFLL 7
           +ATNF+L
Sbjct: 399 VATNFML 405
>NP_009099
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LATNFLL 7
           +ATNF+L
Sbjct: 399 VATNFML 405
>NP_758519
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATNFLLK 8
           AT FLLK
Sbjct: 485 ATGFLLK 491
>NP_000751
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATNFLLK 8
           AT FLLK
Sbjct: 485 ATGFLLK 491
>NP_724781
          Length = 863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATNFLLK 8
           AT FLLK
Sbjct: 485 ATGFLLK 491
>NP_940999
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LATNFLLK 8
           + TNFL+K
Sbjct: 357 VTTNFLVK 364
>XP_006721800
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 360 NFLMKM 365
>XP_005257167
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 867 NFLMKM 872
>XP_005256997
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LATNFLL 7
           +ATNF+L
Sbjct: 338 VATNFML 344
>XP_005256995
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LATNFLL 7
           +ATNF+L
Sbjct: 399 VATNFML 405
>XP_005251551
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NF+LKM
Sbjct: 815 NFILKM 820
>XP_006716857
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NF+LKM
Sbjct: 848 NFILKM 853
>XP_006712810
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 115 NFLIKM 120
>XP_006712809
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 115 NFLIKM 120
>XP_006712808
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NFLLKM 9
           NFL+KM
Sbjct: 115 NFLIKM 120
>XP_005270552
          Length = 731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATNFLLK 8
           AT FLLK
Sbjct: 353 ATGFLLK 359
>XP_005270550
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATNFLLK 8
           AT FLLK
Sbjct: 485 ATGFLLK 491
>XP_005270549
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATNFLLK 8
           AT FLLK
Sbjct: 463 ATGFLLK 469
>NP_004913
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LATNFLLK 8
           + TNFL+K
Sbjct: 357 VTTNFLVK 364
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.142    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,532
Number of extensions: 532
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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