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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001077415                                                           18    1482
XP_005264468                                                           18    1482
XP_005264467                                                           18    1482
NP_653337                                                              18    1482
NP_444256                                                              18    1935
NP_444254                                                              18    1935
NP_444253                                                              18    1935
NP_444255                                                              18    1935
XP_005247551                                                           18    1935
XP_005247550                                                           18    1935
XP_006713712                                                           18    1935
XP_005247549                                                           18    1935
NP_071394                                                              17    4312
NP_001177410                                                           17    4312
NP_997244                                                              16    5631
NP_001034656                                                           16    5631
NP_001278413                                                           16    7355
NP_004354                                                              16    7355
NP_612154                                                              16    7355
NP_060876                                                              16    7355
NP_004523                                                              16    7355
XP_005258472                                                           16    7355
NP_573572                                                              15    9605
NP_001106962                                                           15    9605
NP_699195                                                              15    9605
NP_001138502                                                           15    9605
NP_001014364                                                           15    9605
NP_060275                                                              15    9605
XP_006724686                                                           15    9605
XP_005262147                                                           15    9605
XP_005262146                                                           15    9605
XP_005262145                                                           15    9605
XP_005262144                                                           15    9605
XP_005259081                                                           15    9605
XP_006723314                                                           15    9605
XP_005259080                                                           15    9605
XP_006723313                                                           15    9605

>NP_001077415
          Length = 343

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LATGRSTSF 9
          +ATG+ST+F
Sbjct: 83 MATGKSTAF 91



>XP_005264468
          Length = 370

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LATGRSTSF 9
           +ATG+ST+F
Sbjct: 110 MATGKSTAF 118



>XP_005264467
          Length = 399

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LATGRSTSF 9
          +ATG+ST+F
Sbjct: 68 MATGKSTAF 76



>NP_653337
          Length = 441

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LATGRSTSF 9
           +ATG+ST+F
Sbjct: 110 MATGKSTAF 118



>NP_444256
          Length = 1794

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1312 LATCRSTSF 1320



>NP_444254
          Length = 1845

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1312 LATCRSTSF 1320



>NP_444253
          Length = 1914

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1381 LATCRSTSF 1389



>NP_444255
          Length = 1863

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1381 LATCRSTSF 1389



>XP_005247551
          Length = 1855

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1322 LATCRSTSF 1330



>XP_005247550
          Length = 1856

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1391 LATCRSTSF 1399



>XP_006713712
          Length = 1923

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1391 LATCRSTSF 1399



>XP_005247549
          Length = 1924

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LATGRSTSF 9
            LAT RSTSF
Sbjct: 1391 LATCRSTSF 1399



>NP_071394
          Length = 1339

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TGRSTSF 9
           TGR TSF
Sbjct: 793 TGRGTSF 799



>NP_001177410
          Length = 1275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TGRSTSF 9
           TGR TSF
Sbjct: 729 TGRGTSF 735



>NP_997244
          Length = 3169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  TGRSTSF 9
          TG STSF
Sbjct: 14 TGNSTSF 20



>NP_001034656
          Length = 263

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LATGRSTSF 9
           L TG+ TSF
Sbjct: 164 LGTGKITSF 172



>NP_001278413
          Length = 702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LATGR+ S
Sbjct: 660 LATGRNNS 667



>NP_004354
          Length = 702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LATGR+ S
Sbjct: 660 LATGRNNS 667



>NP_612154
          Length = 1125

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ATGRSTSF 9
           +TGR T+F
Sbjct: 118 STGRGTTF 125



>NP_060876
          Length = 5412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ATGRSTSF 9
            +TGR T+F
Sbjct: 4405 STGRGTTF 4412



>NP_004523
          Length = 1176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ATGRSTSF 9
           +TGR T+F
Sbjct: 169 STGRGTTF 176



>XP_005258472
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LATGR+ S
Sbjct: 378 LATGRNNS 385



>NP_573572
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 149 LATARSTN 156



>NP_001106962
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 558 LATARSTN 565



>NP_699195
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LATGRS 6
           LATGRS
Sbjct: 142 LATGRS 147



>NP_001138502
          Length = 1692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LATGRSTSF 9
           L TGR T+F
Sbjct: 679 LNTGRLTNF 687



>NP_001014364
          Length = 2391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LATGRSTSF 9
           L++G+S+SF
Sbjct: 724 LSSGQSSSF 732



>NP_060275
          Length = 963

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 165 LATARSTA 172



>XP_006724686
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 149 LATARSTN 156



>XP_005262147
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 149 LATARSTN 156



>XP_005262146
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 558 LATARSTN 565



>XP_005262145
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 558 LATARSTN 565



>XP_005262144
          Length = 1084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 558 LATARSTN 565



>XP_005259081
          Length = 966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 266 LATARSTA 273



>XP_006723314
          Length = 968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 266 LATARSTA 273



>XP_005259080
          Length = 1064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 266 LATARSTA 273



>XP_006723313
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LATGRSTS 8
           LAT RST+
Sbjct: 266 LATARSTA 273



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.122    0.321 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 450,482
Number of extensions: 567
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)