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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_075069                                                              16    7355
NP_001273205                                                           16    7355
NP_001162195                                                           16    7355
NP_001273203                                                           16    7355
NP_065982                                                              16    7355
NP_001273204                                                           16    7355
XP_006718731                                                           16    7355
XP_006718730                                                           16    7355
NP_056502                                                              15    9605
NP_001276916                                                           15    9605
XP_005248772                                                           15    9605
XP_005248771                                                           15    9605
XP_005248770                                                           15    9605

>NP_075069
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LASKIVSTV 9
           LASK+V T+
Sbjct: 353 LASKLVETM 361



>NP_001273205
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASKIVSTV 9
           A KIVST+
Sbjct: 626 AKKIVSTI 633



>NP_001162195
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LASKIVSTV 9
           LASK+V T+
Sbjct: 331 LASKLVETM 339



>NP_001273203
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASKIVSTV 9
           A KIVST+
Sbjct: 666 AKKIVSTI 673



>NP_065982
          Length = 787

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASKIVSTV 9
           A KIVST+
Sbjct: 693 AKKIVSTI 700



>NP_001273204
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASKIVSTV 9
           A KIVST+
Sbjct: 626 AKKIVSTI 633



>XP_006718731
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LASKIVSTV 9
           LASK+V T+
Sbjct: 225 LASKLVETM 233



>XP_006718730
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LASKIVSTV 9
           LASK+V T+
Sbjct: 238 LASKLVETM 246



>NP_056502
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ASKIVSTV 9
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



>NP_001276916
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ASKIVSTV 9
            ASK+ ST+
Sbjct: 1125 ASKVTSTI 1132



>XP_005248772
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ASKIVSTV 9
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



>XP_005248771
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ASKIVSTV 9
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



>XP_005248770
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ASKIVSTV 9
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.119    0.257 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 196,532
Number of extensions: 107
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)