Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              20     390
XP_006714104                                                           20     390
XP_005265800                                                           20     390
XP_006714103                                                           20     390
XP_006714102                                                           20     390
NP_115984                                                              17    3301
NP_001273336                                                           17    4312
NP_689473                                                              17    4312
NP_000740                                                              16    5631
NP_055429                                                              16    5631
NP_660281                                                              16    5631
XP_006712032                                                           16    5631
XP_006710939                                                           16    5631
XP_005271228                                                           16    5631
XP_006710938                                                           16    5631
XP_006710937                                                           16    5631
NP_004817                                                              16    7355
NP_001277716                                                           16    7355
NP_061848                                                              16    7355
NP_036553                                                              16    7355
NP_006044                                                              15    9605
NP_006010                                                              15    9605
NP_001539                                                              15    9605
NP_001257856                                                           15    9605
NP_006693                                                              15    9605
NP_001159583                                                           15    9605
NP_001121613                                                           15    9605
NP_001159585                                                           15    9605
NP_001159586                                                           15    9605
NP_001159584                                                           15    9605
NP_001257859                                                           15    9605
NP_001257857                                                           15    9605
NP_001257858                                                           15    9605
XP_005262372                                                           15    9605
XP_005262371                                                           15    9605
XP_006721223                                                           15    9605
XP_005256343                                                           15    9605
XP_005256342                                                           15    9605
XP_006721222                                                           15    9605
XP_006718480                                                           15    9605
XP_005273766                                                           15    9605
NP_004404                                                              15    9605
>NP_006197
          Length = 1089

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAQNEDAVK 9
           +AQNEDAVK
Sbjct: 395 VAQNEDAVK 403
>XP_006714104
          Length = 832

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAQNEDAVK 9
           +AQNEDAVK
Sbjct: 420 VAQNEDAVK 428
>XP_005265800
          Length = 1089

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAQNEDAVK 9
           +AQNEDAVK
Sbjct: 395 VAQNEDAVK 403
>XP_006714103
          Length = 1111

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAQNEDAVK 9
           +AQNEDAVK
Sbjct: 417 VAQNEDAVK 425
>XP_006714102
          Length = 1114

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LAQNEDAVK 9
           +AQNEDAVK
Sbjct: 420 VAQNEDAVK 428
>NP_115984
          Length = 817

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LAQNEDAV 8
           LA+NEDA+
Sbjct: 650 LAENEDAL 657
>NP_001273336
          Length = 484

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LAQNEDAV 8
           LA+NED V
Sbjct: 165 LAENEDVV 172
>NP_689473
          Length = 545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LAQNEDAV 8
           LA+NED V
Sbjct: 226 LAENEDVV 233
>NP_000740
          Length = 458

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LAQNEDAV 8
          +A+NEDA+
Sbjct: 25 IAENEDAL 32
>NP_055429
          Length = 1843

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    NEDAVK 9
            NEDAVK
Sbjct: 1679 NEDAVK 1684
>NP_660281
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDAVK
Sbjct: 608 NEDAVK 613
>XP_006712032
          Length = 1001

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDAVK
Sbjct: 837 NEDAVK 842
>XP_006710939
          Length = 989

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDAVK
Sbjct: 554 NEDAVK 559
>XP_005271228
          Length = 1042

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDAVK
Sbjct: 607 NEDAVK 612
>XP_006710938
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDAVK
Sbjct: 608 NEDAVK 613
>XP_006710937
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDAVK
Sbjct: 608 NEDAVK 613
>NP_004817
          Length = 775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LAQNEDAVK 9
           LAQ+ED+ K
Sbjct: 277 LAQDEDSEK 285
>NP_001277716
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LAQNEDAVK 9
           LAQ+ED+ K
Sbjct: 277 LAQDEDSEK 285
>NP_061848
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LAQNEDAVK 9
           + +NE+AVK
Sbjct: 243 IQENEEAVK 251
>NP_036553
          Length = 1914

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEDAVK 9
           NEDA+K
Sbjct: 264 NEDAIK 269
>NP_006044
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAQNEDAVK 9
           LA+N  AVK
Sbjct: 206 LAENRPAVK 214
>NP_006010
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAQNEDAVK 9
           LA+N  AVK
Sbjct: 422 LAENRPAVK 430
>NP_001539
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QNE+A+K
Sbjct: 67 QNEEALK 73
>NP_001257856
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QNE+A+K
Sbjct: 67 QNEEALK 73
>NP_006693
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LAQNEDA 7
           LAQ EDA
Sbjct: 794 LAQQEDA 800
>NP_001159583
          Length = 1375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LAQNEDA 7
           LAQ EDA
Sbjct: 842 LAQQEDA 848
>NP_001121613
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QN+DAV+
Sbjct: 90 QNKDAVR 96
>NP_001159585
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LAQNEDA 7
           LAQ EDA
Sbjct: 794 LAQQEDA 800
>NP_001159586
          Length = 1365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LAQNEDA 7
           LAQ EDA
Sbjct: 832 LAQQEDA 838
>NP_001159584
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LAQNEDA 7
           LAQ EDA
Sbjct: 767 LAQQEDA 773
>NP_001257859
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QNE+A+K
Sbjct: 36 QNEEALK 42
>NP_001257857
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QNE+A+K
Sbjct: 36 QNEEALK 42
>NP_001257858
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QNE+A+K
Sbjct: 36 QNEEALK 42
>XP_005262372
          Length = 1027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LAQNEDA 7
          L+QNED+
Sbjct: 21 LSQNEDS 27
>XP_005262371
          Length = 1107

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LAQNEDA 7
           L+QNED+
Sbjct: 101 LSQNEDS 107
>XP_006721223
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QNEDAVK 9
           QN+DAV+
Sbjct: 125 QNKDAVR 131
>XP_005256343
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QN+DAV+
Sbjct: 90 QNKDAVR 96
>XP_005256342
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QN+DAV+
Sbjct: 90 QNKDAVR 96
>XP_006721222
          Length = 446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QNEDAVK 9
           QN+DAV+
Sbjct: 125 QNKDAVR 131
>XP_006718480
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAQNEDAVK 9
           LA+N  AVK
Sbjct: 422 LAENRPAVK 430
>XP_005273766
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAQNEDAVK 9
           LA+N  AVK
Sbjct: 422 LAENRPAVK 430
>NP_004404
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNEDAVK 9
          QN+DAV+
Sbjct: 90 QNKDAVR 96
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.116    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 546,553
Number of extensions: 516
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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