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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060264                                                              17    3301
XP_005251551                                                           17    3301
XP_006716857                                                           17    3301
NP_085048                                                              16    5631
NP_055221                                                              15    9605
NP_001159450                                                           15    9605
NP_653308                                                              15    9605
NP_001159449                                                           15    9605
NP_060743                                                              15    9605
XP_005263936                                                           15    9605

>NP_060264
          Length = 1801

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LALSSLAAV 9
           LALSSLA V
Sbjct: 962 LALSSLAVV 970



>XP_005251551
          Length = 1801

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LALSSLAAV 9
           LALSSLA V
Sbjct: 962 LALSSLAVV 970



>XP_006716857
          Length = 1834

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LALSSLAAV 9
            LALSSLA V
Sbjct: 995  LALSSLAVV 1003



>NP_085048
          Length = 221

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  LALSSLAAV 9
          LAL++LAAV
Sbjct: 3  LALAALAAV 11



>NP_055221
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  LSSLAAV 9
          LSSLAAV
Sbjct: 34 LSSLAAV 40



>NP_001159450
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LALSSLAA 8
          LALS LAA
Sbjct: 18 LALSQLAA 25



>NP_653308
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LALSSLAA 8
          LALS LAA
Sbjct: 18 LALSQLAA 25



>NP_001159449
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LALSSLAA 8
          LALS LAA
Sbjct: 18 LALSQLAA 25



>NP_060743
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2  ALSSLAA 8
          ALSSLAA
Sbjct: 43 ALSSLAA 49



>XP_005263936
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LALSSLAA 8
          LALS LAA
Sbjct: 18 LALSQLAA 25



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.120    0.254 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 223,365
Number of extensions: 248
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)