Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1135
NP_009297                                                              18    1135
NP_001271141                                                           17    4312
NP_001271142                                                           17    4312
NP_775083                                                              17    4312
NP_001035905                                                           17    4312
NP_001177371                                                           17    4312
XP_006714775                                                           17    4312
XP_006714774                                                           17    4312
XP_006714773                                                           17    4312
XP_006714772                                                           17    4312
XP_006714771                                                           17    4312
XP_006714770                                                           17    4312
XP_006714769                                                           17    4312
XP_006714768                                                           17    4312
XP_006714767                                                           17    4312
XP_006714766                                                           17    4312
XP_006714765                                                           17    4312
XP_006714764                                                           17    4312
XP_006714763                                                           17    4312
XP_006714762                                                           17    4312
XP_006714761                                                           17    4312
XP_006714760                                                           17    4312
XP_006714759                                                           17    4312
NP_001139                                                              16    5631
XP_005259184                                                           16    5631
XP_006714257                                                           16    5631
XP_005263002                                                           16    5631
XP_005262999                                                           16    5631
XP_006714252                                                           16    5631
XP_006714251                                                           16    5631
XP_006714250                                                           16    5631
NP_659491                                                              16    5631
NP_000490                                                              16    7355
NP_689914                                                              16    7355
NP_006500                                                              16    7355
XP_005259185                                                           16    7355
XP_005254242                                                           16    7355
NP_076985                                                              15    9605
>NP_005148
          Length = 1130

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LAISRSSEQ 9
            LAISR+SEQ
Sbjct: 1041 LAISRNSEQ 1049
>NP_009297
          Length = 1149

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LAISRSSEQ 9
            LAISR+SEQ
Sbjct: 1060 LAISRNSEQ 1068
>NP_001271141
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 42 AVSRSAEQ 49
>NP_001271142
          Length = 636

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 61 AVSRSAEQ 68
>NP_775083
          Length = 686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 61 AVSRSAEQ 68
>NP_001035905
          Length = 750

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 125 AVSRSAEQ 132
>NP_001177371
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 83 AVSRSAEQ 90
>XP_006714775
          Length = 654

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 42 AVSRSAEQ 49
>XP_006714774
          Length = 667

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 42 AVSRSAEQ 49
>XP_006714773
          Length = 673

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 61 AVSRSAEQ 68
>XP_006714772
          Length = 689

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 64 AVSRSAEQ 71
>XP_006714771
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 83 AVSRSAEQ 90
>XP_006714770
          Length = 708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AISRSSEQ 9
          A+SRS+EQ
Sbjct: 83 AVSRSAEQ 90
>XP_006714769
          Length = 735

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 110 AVSRSAEQ 117
>XP_006714768
          Length = 749

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 125 AVSRSAEQ 132
>XP_006714767
          Length = 754

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 129 AVSRSAEQ 136
>XP_006714766
          Length = 756

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 144 AVSRSAEQ 151
>XP_006714765
          Length = 757

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 132 AVSRSAEQ 139
>XP_006714764
          Length = 769

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 144 AVSRSAEQ 151
>XP_006714763
          Length = 772

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 147 AVSRSAEQ 154
>XP_006714762
          Length = 776

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 151 AVSRSAEQ 158
>XP_006714761
          Length = 778

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 166 AVSRSAEQ 173
>XP_006714760
          Length = 790

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 166 AVSRSAEQ 173
>XP_006714759
          Length = 791

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AISRSSEQ 9
           A+SRS+EQ
Sbjct: 166 AVSRSAEQ 173
>NP_001139
          Length = 3957

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1669 LAVGRSSEK 1677
>XP_005259184
          Length = 576

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LAISRSSE 8
          LA+SRS+E
Sbjct: 45 LAVSRSTE 52
>XP_006714257
          Length = 4145

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1678 LAVGRSSEK 1686
>XP_005263002
          Length = 4072

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1636 LAVGRSSEK 1644
>XP_005262999
          Length = 4147

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1680 LAVGRSSEK 1688
>XP_006714252
          Length = 4153

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1686 LAVGRSSEK 1694
>XP_006714251
          Length = 4165

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1698 LAVGRSSEK 1706
>XP_006714250
          Length = 4190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LAISRSSEQ 9
            LA+ RSSE+
Sbjct: 1723 LAVGRSSEK 1731
>NP_659491
          Length = 620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  LAISRSSE 8
          LAISRS E
Sbjct: 70 LAISRSGE 77
>NP_000490
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LAISRSSEQ 9
          LA+SR S+Q
Sbjct: 61 LALSRMSQQ 69
>NP_689914
          Length = 5058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    AISRSSEQ 9
            AISR+SE+
Sbjct: 2481 AISRASEE 2488
>NP_006500
          Length = 579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  LAISRSSEQ 9
          LA+SRS+++
Sbjct: 45 LAVSRSTDE 53
>XP_005259185
          Length = 507

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  LAISRSSEQ 9
          LA+SRS+++
Sbjct: 45 LAVSRSTDE 53
>XP_005254242
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LAISRSSEQ 9
          LA+SR S+Q
Sbjct: 61 LALSRMSQQ 69
>NP_076985
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISRSSEQ 9
           ISRSSE+
Sbjct: 201 ISRSSEE 207
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.108    0.226 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 248,217
Number of extensions: 254
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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