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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_071401                                                              17    4312
NP_001273561                                                           17    4312
NP_001273560                                                           17    4312
XP_006719923                                                           17    4312
XP_006719922                                                           17    4312
XP_005266558                                                           17    4312
XP_006719921                                                           17    4312
XP_005266556                                                           17    4312
XP_005266555                                                           17    4312
XP_006719920                                                           17    4312
XP_005266554                                                           17    4312
XP_005266553                                                           17    4312
XP_006719919                                                           17    4312
XP_005266551                                                           17    4312
XP_005266550                                                           17    4312
XP_005266549                                                           17    4312
XP_006719918                                                           17    4312
XP_005266548                                                           17    4312
NP_002386                                                              16    5631
XP_005255097                                                           16    5631
NP_004095                                                              16    7355
NP_001025054                                                           16    7355
NP_060232                                                              16    7355
NP_055704                                                              16    7355
NP_001229236                                                           16    7355
NP_001229238                                                           16    7355
XP_006725610                                                           16    7355
XP_006725608                                                           16    7355
XP_005254565                                                           16    7355
XP_006720651                                                           16    7355
XP_006717425                                                           16    7355
XP_005252402                                                           16    7355
NP_001161807                                                           15    9605
NP_002387                                                              15    9605

>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LAFTASDRQ 9
           LA TASDRQ
Sbjct: 917 LALTASDRQ 925



>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LAFTASDRQ 9
           LA TASDRQ
Sbjct: 917 LALTASDRQ 925



>NP_071401
          Length = 980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 870 AFTESDRE 877



>NP_001273561
          Length = 983

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 873 AFTESDRE 880



>NP_001273560
          Length = 1009

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 899 AFTESDRE 906



>XP_006719923
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 624 AFTESDRE 631



>XP_006719922
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 912 AFTESDRE 919



>XP_005266558
          Length = 980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 870 AFTESDRE 877



>XP_006719921
          Length = 983

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 873 AFTESDRE 880



>XP_005266556
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 885 AFTESDRE 892



>XP_005266555
          Length = 998

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 888 AFTESDRE 895



>XP_006719920
          Length = 999

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 912 AFTESDRE 919



>XP_005266554
          Length = 1000

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 912 AFTESDRE 919



>XP_005266553
          Length = 1007

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 897 AFTESDRE 904



>XP_006719919
          Length = 1009

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 899 AFTESDRE 906



>XP_005266551
          Length = 1012

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 902 AFTESDRE 909



>XP_005266550
          Length = 1017

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 907 AFTESDRE 914



>XP_005266549
          Length = 1019

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 909 AFTESDRE 916



>XP_006719918
          Length = 1021

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 911 AFTESDRE 918



>XP_005266548
          Length = 1022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AFTASDRQ 9
           AFT SDR+
Sbjct: 912 AFTESDRE 919



>NP_002386
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LAFTASDRQ 9
          LAFT  +RQ
Sbjct: 28 LAFTLEERQ 36



>XP_005255097
          Length = 287

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 LAFTASDR 8
         +AFTAS R
Sbjct: 1 MAFTASQR 8



>NP_004095
          Length = 2511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LAFTASDR 8
            L++TA+DR
Sbjct: 1268 LSYTATDR 1275



>NP_001025054
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    TASDRQ 9
            TASDRQ
Sbjct: 1193 TASDRQ 1198



>NP_060232
          Length = 777

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LAFTASDR 8
           L FTA DR
Sbjct: 542 LQFTAKDR 549



>NP_055704
          Length = 1037

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFTASD 7
           AFTASD
Sbjct: 137 AFTASD 142



>NP_001229236
          Length = 1038

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFTASD 7
           AFTASD
Sbjct: 137 AFTASD 142



>NP_001229238
          Length = 939

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFTASD 7
           AFTASD
Sbjct: 105 AFTASD 110



>XP_006725610
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LAFTASDR 8
           L FTA DR
Sbjct: 294 LQFTAKDR 301



>XP_006725608
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LAFTASDR 8
           L FTA DR
Sbjct: 542 LQFTAKDR 549



>XP_005254565
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LAFTASDR 8
           L FTA DR
Sbjct: 294 LQFTAKDR 301



>XP_006720651
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LAFTASDR 8
           L FTA DR
Sbjct: 542 LQFTAKDR 549



>XP_006717425
          Length = 867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFTASD 7
           AFTASD
Sbjct: 137 AFTASD 142



>XP_005252402
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AFTASD 7
           AFTASD
Sbjct: 137 AFTASD 142



>NP_001161807
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LAFTASDRQ 9
          +AFT  +RQ
Sbjct: 38 MAFTLQERQ 46



>NP_002387
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LAFTASDRQ 9
          +AFT  +RQ
Sbjct: 38 MAFTLQERQ 46



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.124    0.322 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 377,281
Number of extensions: 418
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)