Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1935
NP_001075109                                                           18    1935
NP_001271169                                                           17    2528
NP_001271171                                                           17    2528
XP_005269965                                                           17    2528
XP_006717958                                                           17    2528
XP_006717957                                                           17    2528
NP_055758                                                              17    4312
NP_945327                                                              17    4312
XP_005250893                                                           17    4312
XP_005250894                                                           17    4312
NP_002378                                                              16    5631
NP_001078846                                                           16    5631
XP_005272048                                                           16    5631
XP_006714680                                                           16    5631
NP_258441                                                              16    5631
NP_001070993                                                           16    7355
XP_005260950                                                           16    7355
XP_005260949                                                           16    7355
XP_005260948                                                           16    7355
XP_005260947                                                           16    7355
XP_005265457                                                           16    7355
NP_001070994                                                           16    7355
NP_001129386                                                           16    7355
NP_588616                                                              15    9605
NP_001137160                                                           15    9605
NP_001229698                                                           15    9605
NP_001229696                                                           15    9605
NP_056255                                                              15    9605
NP_005483                                                              15    9605
NP_001268659                                                           15    9605
XP_005260696                                                           15    9605
XP_005254203                                                           15    9605
XP_005254204                                                           15    9605
XP_005254202                                                           15    9605
XP_005254201                                                           15    9605
XP_005266968                                                           15    9605
XP_005266967                                                           15    9605
XP_005266964                                                           15    9605
XP_006715114                                                           15    9605
XP_006715113                                                           15    9605
NP_036479                                                              15    9605
>NP_008835
          Length = 4128

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LAEARSDCS 9
            LAEARSD S
Sbjct: 2958 LAEARSDYS 2966
>NP_001075109
          Length = 4097

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LAEARSDCS 9
            LAEARSD S
Sbjct: 2958 LAEARSDYS 2966
>NP_001271169
          Length = 1047

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EARSDCS 9
           EARS+CS
Sbjct: 803 EARSECS 809
>NP_001271171
          Length = 474

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EARSDCS 9
           EARS+CS
Sbjct: 230 EARSECS 236
>XP_005269965
          Length = 474

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EARSDCS 9
           EARS+CS
Sbjct: 230 EARSECS 236
>XP_006717958
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EARSDCS 9
           EARS+CS
Sbjct: 249 EARSECS 255
>XP_006717957
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EARSDCS 9
           EARS+CS
Sbjct: 249 EARSECS 255
>NP_055758
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LAEARSDC 8
           LAE+ SDC
Sbjct: 822 LAESDSDC 829
>NP_945327
          Length = 599

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LAEARSDC 8
           LAEA  DC
Sbjct: 171 LAEAHPDC 178
>XP_005250893
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LAEARSDC 8
           LAE+ SDC
Sbjct: 822 LAESDSDC 829
>XP_005250894
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LAEARSDC 8
           LAE+ SDC
Sbjct: 822 LAESDSDC 829
>NP_002378
          Length = 829

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAE R+ CS
Sbjct: 707 LAELRTTCS 715
>NP_001078846
          Length = 1019

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAE R+ CS
Sbjct: 897 LAELRTTCS 905
>XP_005272048
          Length = 851

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAE R+ CS
Sbjct: 729 LAELRTTCS 737
>XP_006714680
          Length = 1002

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAE R+ CS
Sbjct: 880 LAELRTTCS 888
>NP_258441
          Length = 437

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1 LAEARSDC 8
         +AEA  DC
Sbjct: 1 MAEAEEDC 8
>NP_001070993
          Length = 650

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAEARS  S
Sbjct: 507 LAEARSSSS 515
>XP_005260950
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LAEARSDCS 9
           LAE R  CS
Sbjct: 364 LAELRQGCS 372
>XP_005260949
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LAEARSDCS 9
           LAE R  CS
Sbjct: 364 LAELRQGCS 372
>XP_005260948
          Length = 682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LAEARSDCS 9
           LAE R  CS
Sbjct: 394 LAELRQGCS 402
>XP_005260947
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LAEARSDCS 9
           LAE R  CS
Sbjct: 394 LAELRQGCS 402
>XP_005265457
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAEARS  S
Sbjct: 482 LAEARSSSS 490
>NP_001070994
          Length = 598

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LAEARSDCS 9
           LAEARS  S
Sbjct: 507 LAEARSSSS 515
>NP_001129386
          Length = 1503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AEARSDCS 9
            AEA S+CS
Sbjct: 1020 AEALSNCS 1027
>NP_588616
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  LAEARSDC 8
          L +AR DC
Sbjct: 24 LKDARHDC 31
>NP_001137160
          Length = 1054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   LAEARSDC 8
           L +AR DC
Sbjct: 107 LKDARHDC 114
>NP_001229698
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EARSDCS 9
          +ARS CS
Sbjct: 56 QARSSCS 62
>NP_001229696
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EARSDCS 9
          +ARS CS
Sbjct: 56 QARSSCS 62
>NP_056255
          Length = 978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EARSDCS 9
          +ARS CS
Sbjct: 56 QARSSCS 62
>NP_005483
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  ARSDC 8
          ARSDC
Sbjct: 91 ARSDC 95
>NP_001268659
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  ARSDC 8
          ARSDC
Sbjct: 91 ARSDC 95
>XP_005260696
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  ARSDC 8
          ARSDC
Sbjct: 91 ARSDC 95
>XP_005254203
          Length = 848

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   LAEARSDC 8
           L +AR DC
Sbjct: 107 LKDARHDC 114
>XP_005254204
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  LAEARSDC 8
          L +AR DC
Sbjct: 24 LKDARHDC 31
>XP_005254202
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  LAEARSDC 8
          L +AR DC
Sbjct: 24 LKDARHDC 31
>XP_005254201
          Length = 1037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  LAEARSDC 8
          L +AR DC
Sbjct: 90 LKDARHDC 97
>XP_005266968
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EARSDCS 9
          +ARS CS
Sbjct: 56 QARSSCS 62
>XP_005266967
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EARSDCS 9
          +ARS CS
Sbjct: 56 QARSSCS 62
>XP_005266964
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EARSDCS 9
          +ARS CS
Sbjct: 56 QARSSCS 62
>XP_006715114
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  AEARSDC 8
          AEA  DC
Sbjct: 12 AEAEEDC 18
>XP_006715113
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  AEARSDC 8
          AEA  DC
Sbjct: 12 AEAEEDC 18
>NP_036479
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  AEARSDC 8
          AEA  DC
Sbjct: 12 AEAEEDC 18
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.117    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 484,204
Number of extensions: 452
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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