Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     390
NP_001275661                                                           18    1135
NP_060939                                                              18    1135
NP_001275662                                                           18    1135
XP_005257564                                                           18    1135
NP_004057                                                              17    4312
XP_005270828                                                           17    4312
NP_113609                                                              16    5631
XP_006722192                                                           16    5631
XP_006714402                                                           16    5631
XP_006714401                                                           16    5631
XP_006714400                                                           16    5631
XP_006714399                                                           16    5631
XP_005263327                                                           16    5631
XP_006714398                                                           16    5631
XP_005263326                                                           16    5631
NP_114162                                                              16    5631
NP_775901                                                              16    7355
NP_689648                                                              16    7355
XP_006726605                                                           16    7355
XP_006722498                                                           16    7355
XP_006722497                                                           16    7355
XP_006719242                                                           16    7355
XP_006718551                                                           16    7355
XP_005273928                                                           16    7355
XP_005248127                                                           16    7355
XP_005248124                                                           16    7355
XP_005248123                                                           16    7355
XP_005248122                                                           16    7355
XP_005248121                                                           16    7355
XP_006714059                                                           16    7355
XP_005248119                                                           16    7355
XP_005248118                                                           16    7355
XP_005248117                                                           16    7355
XP_005248116                                                           16    7355
XP_005248115                                                           16    7355
XP_005248114                                                           16    7355
XP_006710022                                                           16    7355
NP_689973                                                              16    7355
NP_004374                                                              15    9605
NP_001120662                                                           15    9605
NP_073739                                                              15    9605
NP_001129628                                                           15    9605
NP_055616                                                              15    9605
NP_055725                                                              15    9605
XP_006726509                                                           15    9605
XP_005254222                                                           15    9605
XP_006717822                                                           15    9605
XP_006717821                                                           15    9605
XP_005269749                                                           15    9605
XP_006717820                                                           15    9605
XP_006716286                                                           15    9605
XP_006711879                                                           15    9605
XP_005273329                                                           15    9605
NP_065081                                                              15    9605
NP_710157                                                              15    9605
>NP_004295
          Length = 1620

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KVSEVGREG 9
           K SEVGREG
Sbjct: 194 KASEVGREG 202
>NP_001275661
          Length = 1127

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KVSEVGREG 9
           KV E+G+EG
Sbjct: 927 KVGEIGKEG 935
>NP_060939
          Length = 1134

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KVSEVGREG 9
           KV E+G+EG
Sbjct: 934 KVGEIGKEG 942
>NP_001275662
          Length = 1127

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KVSEVGREG 9
           KV E+G+EG
Sbjct: 927 KVGEIGKEG 935
>XP_005257564
          Length = 1125

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KVSEVGREG 9
           KV E+G+EG
Sbjct: 927 KVGEIGKEG 935
>NP_004057
          Length = 172

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VSEVGREG 9
          +SEV REG
Sbjct: 73 ISEVDREG 80
>XP_005270828
          Length = 444

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EVGREG 9
          EVGREG
Sbjct: 21 EVGREG 26
>NP_113609
          Length = 672

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KVSEVGR 7
           K+SEVGR
Sbjct: 637 KLSEVGR 643
>XP_006722192
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KVSEVGR 7
           K+SEVGR
Sbjct: 474 KLSEVGR 480
>XP_006714402
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 223 KASEIAKEG 231
>XP_006714401
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 259 KASEIAKEG 267
>XP_006714400
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 285 KASEIAKEG 293
>XP_006714399
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 285 KASEIAKEG 293
>XP_005263327
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 285 KASEIAKEG 293
>XP_006714398
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 285 KASEIAKEG 293
>XP_005263326
          Length = 501

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 285 KASEIAKEG 293
>NP_114162
          Length = 475

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K SE+ +EG
Sbjct: 259 KASEIAKEG 267
>NP_775901
          Length = 2226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VSEVGREG 9
           +S++G+EG
Sbjct: 557 LSDIGKEG 564
>NP_689648
          Length = 426

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           K+ E+GR G
Sbjct: 214 KLKELGRNG 222
>XP_006726605
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEVGRE 8
           KVSE+G E
Sbjct: 142 KVSEMGSE 149
>XP_006722498
          Length = 2059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VSEVGREG 9
           +S++G+EG
Sbjct: 557 LSDIGKEG 564
>XP_006722497
          Length = 2227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VSEVGREG 9
           +S++G+EG
Sbjct: 557 LSDIGKEG 564
>XP_006719242
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEVGRE 8
           KVSE+G E
Sbjct: 142 KVSEMGSE 149
>XP_006718551
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SEVGREG 9
          +EVG+EG
Sbjct: 5  AEVGKEG 11
>XP_005273928
          Length = 415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SEVGREG 9
          +EVG+EG
Sbjct: 5  AEVGKEG 11
>XP_005248127
          Length = 1322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248124
          Length = 1467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 418 KVSEKARDG 426
>XP_005248123
          Length = 1471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 418 KVSEKARDG 426
>XP_005248122
          Length = 1471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 418 KVSEKARDG 426
>XP_005248121
          Length = 1543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 490 KVSEKARDG 498
>XP_006714059
          Length = 1600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248119
          Length = 1601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248118
          Length = 1602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248117
          Length = 1626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248116
          Length = 1627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248115
          Length = 1629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_005248114
          Length = 1630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEVGREG 9
           KVSE  R+G
Sbjct: 577 KVSEKARDG 585
>XP_006710022
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEVGRE 8
           KVSE+G E
Sbjct: 142 KVSEMGSE 149
>NP_689973
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SEVGREG 9
          +EVG+EG
Sbjct: 5  AEVGKEG 11
>NP_004374
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SEVGREG 9
          ++VGREG
Sbjct: 52 NKVGREG 58
>NP_001120662
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SEVGREG 9
          ++VGREG
Sbjct: 52 NKVGREG 58
>NP_073739
          Length = 1430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SEVGRE 8
            SE+GRE
Sbjct: 1350 SEIGRE 1355
>NP_001129628
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SEVGREG 9
           +++GREG
Sbjct: 431 AQIGREG 437
>NP_055616
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SEVGREG 9
           S+VG+EG
Sbjct: 504 SKVGKEG 510
>NP_055725
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SEVGREG 9
           +++GREG
Sbjct: 431 AQIGREG 437
>XP_006726509
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  VSEVGRE 8
          +SE+G+E
Sbjct: 78 ISEIGKE 84
>XP_005254222
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SEVGREG 9
          ++VGREG
Sbjct: 52 NKVGREG 58
>XP_006717822
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVGREG 9
           EVG+EG
Sbjct: 682 EVGKEG 687
>XP_006717821
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVGREG 9
           EVG+EG
Sbjct: 682 EVGKEG 687
>XP_005269749
          Length = 901

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVGREG 9
           EVG+EG
Sbjct: 682 EVGKEG 687
>XP_006717820
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVGREG 9
           EVG+EG
Sbjct: 682 EVGKEG 687
>XP_006716286
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  VSEVGRE 8
          +SE+G+E
Sbjct: 78 ISEIGKE 84
>XP_006711879
          Length = 1364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SEVGREG 9
           S+VG+EG
Sbjct: 504 SKVGKEG 510
>XP_005273329
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SEVGREG 9
           S+VG+EG
Sbjct: 301 SKVGKEG 307
>NP_065081
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVGREG 9
           EVG+EG
Sbjct: 682 EVGKEG 687
>NP_710157
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SEVGREG 9
           SE GR+G
Sbjct: 100 SEAGRQG 106
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.129    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 472,602
Number of extensions: 587
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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