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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_757374                                                              17    4312
NP_001171618                                                           17    4312
NP_000750                                                              17    4312
NP_757373                                                              17    4312
NP_001273005                                                           15    9605
NP_002937                                                              15    9605
XP_005246022                                                           15    9605

>NP_757374
          Length = 171

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VASRLQSF 9
           VAS LQSF
Sbjct: 150 VASHLQSF 157



>NP_001171618
          Length = 168

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VASRLQSF 9
           VAS LQSF
Sbjct: 147 VASHLQSF 154



>NP_000750
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VASRLQSF 9
           VAS LQSF
Sbjct: 186 VASHLQSF 193



>NP_757373
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VASRLQSF 9
           VAS LQSF
Sbjct: 183 VASHLQSF 190



>NP_001273005
          Length = 174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KVASRLQSF 9
          KVA  L+SF
Sbjct: 31 KVAGHLRSF 39



>NP_002937
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVASRLQSF 9
           KVA  L+SF
Sbjct: 127 KVAGHLRSF 135



>XP_005246022
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVASRLQSF 9
           KVA  L+SF
Sbjct: 135 KVAGHLRSF 143



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.125    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 255,515
Number of extensions: 353
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)