Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           20     390
NP_036462                                                              20     390
NP_001243397                                                           20     390
XP_005269721                                                           20     390
NP_115622                                                              17    3301
NP_037458                                                              16    5631
XP_006722508                                                           16    5631
NP_056091                                                              16    7355
NP_036538                                                              16    7355
XP_005259087                                                           16    7355
XP_006723320                                                           16    7355
XP_005259086                                                           16    7355
XP_006723319                                                           16    7355
XP_005259085                                                           16    7355
XP_005256632                                                           16    7355
XP_006719268                                                           16    7355
XP_005268650                                                           16    7355
NP_808881                                                              16    7355
NP_060498                                                              16    7355
NP_919253                                                              15    9605
NP_001019626                                                           15    9605
NP_958357                                                              15    9605
XP_005262550                                                           15    9605
XP_005271318                                                           15    9605
XP_005271317                                                           15    9605
XP_005271314                                                           15    9605
>NP_001243398
          Length = 1781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KTSNANIGL 9
            +TSNANIGL
Sbjct: 1494 ETSNANIGL 1502
>NP_036462
          Length = 2073

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KTSNANIGL 9
            +TSNANIGL
Sbjct: 1786 ETSNANIGL 1794
>NP_001243397
          Length = 1890

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KTSNANIGL 9
            +TSNANIGL
Sbjct: 1603 ETSNANIGL 1611
>XP_005269721
          Length = 2073

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KTSNANIGL 9
            +TSNANIGL
Sbjct: 1786 ETSNANIGL 1794
>NP_115622
          Length = 569

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SNANIGL 9
           SNAN+GL
Sbjct: 420 SNANLGL 426
>NP_037458
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KTSNANI 7
           KT NANI
Sbjct: 107 KTKNANI 113
>XP_006722508
          Length = 609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KTSNANI 7
           KT NANI
Sbjct: 107 KTKNANI 113
>NP_056091
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TSNANIGL 9
           TSN  IGL
Sbjct: 168 TSNCTIGL 175
>NP_036538
          Length = 801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SN N+GL
Sbjct: 152 SNGNVGL 158
>XP_005259087
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SNAN GL
Sbjct: 218 SNANTGL 224
>XP_006723320
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SNAN GL
Sbjct: 218 SNANTGL 224
>XP_005259086
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SNAN GL
Sbjct: 218 SNANTGL 224
>XP_006723319
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SNAN GL
Sbjct: 218 SNANTGL 224
>XP_005259085
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SNAN GL
Sbjct: 218 SNANTGL 224
>XP_005256632
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  TSNANIGL 9
          TSN  IGL
Sbjct: 63 TSNCTIGL 70
>XP_006719268
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SN N+GL
Sbjct: 152 SNGNVGL 158
>XP_005268650
          Length = 808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SN N+GL
Sbjct: 152 SNGNVGL 158
>NP_808881
          Length = 609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SNANIGL 9
           SNA+IGL
Sbjct: 238 SNADIGL 244
>NP_060498
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SNAN GL
Sbjct: 218 SNANTGL 224
>NP_919253
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SN N+GL
Sbjct: 561 SNINVGL 567
>NP_001019626
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  NANIGL 9
          NAN+GL
Sbjct: 29 NANLGL 34
>NP_958357
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KTSNANIG 8
           KTSN N G
Sbjct: 127 KTSNMNKG 134
>XP_005262550
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SNANIGL 9
           SN N+GL
Sbjct: 546 SNINVGL 552
>XP_005271318
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KTSNANIG 8
           KTSN N G
Sbjct: 127 KTSNMNKG 134
>XP_005271317
          Length = 1368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KTSNANIG 8
           KTSN N G
Sbjct: 127 KTSNMNKG 134
>XP_005271314
          Length = 1368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KTSNANIG 8
           KTSN N G
Sbjct: 127 KTSNMNKG 134
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.119    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,724
Number of extensions: 519
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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