Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     869
NP_671714                                                              19     869
XP_006715890                                                           19     869
XP_005250160                                                           19     869
XP_005250159                                                           19     869
NP_056993                                                              17    3301
NP_055839                                                              17    3301
NP_848622                                                              16    7355
NP_001139                                                              16    7355
XP_006714257                                                           16    7355
XP_005263002                                                           16    7355
XP_005262999                                                           16    7355
XP_006714252                                                           16    7355
XP_006714251                                                           16    7355
XP_006714250                                                           16    7355
XP_006711533                                                           16    7355
XP_005245422                                                           16    7355
NP_065821                                                              16    7355
NP_001073950                                                           15    9605
NP_665804                                                              15    9605
NP_055309                                                              15    9605
XP_006721103                                                           15    9605
XP_005255314                                                           15    9605
XP_006721102                                                           15    9605
XP_005255313                                                           15    9605
XP_005255312                                                           15    9605
XP_006721101                                                           15    9605
XP_005255311                                                           15    9605
XP_005255310                                                           15    9605
XP_005267232                                                           15    9605
XP_005267230                                                           15    9605
XP_005267229                                                           15    9605
XP_005267228                                                           15    9605
XP_005267227                                                           15    9605
XP_005267226                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            K ESTTRLQ
Sbjct: 2237 KKESTTRLQ 2245
>NP_671714
          Length = 3899

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            K ESTTRLQ
Sbjct: 2229 KKESTTRLQ 2237
>XP_006715890
          Length = 3860

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            K ESTTRLQ
Sbjct: 2190 KKESTTRLQ 2198
>XP_005250160
          Length = 3939

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            K ESTTRLQ
Sbjct: 2269 KKESTTRLQ 2277
>XP_005250159
          Length = 3952

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            K ESTTRLQ
Sbjct: 2282 KKESTTRLQ 2290
>NP_056993
          Length = 2371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KTESTTRL 8
            KTE+TT+L
Sbjct: 1026 KTEATTKL 1033
>NP_055839
          Length = 1087

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  TESTTRLQ 9
          T++TTRLQ
Sbjct: 22 TDTTTRLQ 29
>NP_848622
          Length = 1124

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KTESTT 6
           KTESTT
Sbjct: 497 KTESTT 502
>NP_001139
          Length = 3957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2266 KTETTTEIR 2274
>XP_006714257
          Length = 4145

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2275 KTETTTEIR 2283
>XP_005263002
          Length = 4072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2233 KTETTTEIR 2241
>XP_005262999
          Length = 4147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2277 KTETTTEIR 2285
>XP_006714252
          Length = 4153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2283 KTETTTEIR 2291
>XP_006714251
          Length = 4165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2295 KTETTTEIR 2303
>XP_006714250
          Length = 4190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KTESTTRLQ 9
            KTE+TT ++
Sbjct: 2320 KTETTTEIR 2328
>XP_006711533
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           KTE  +RLQ
Sbjct: 399 KTEECSRLQ 407
>XP_005245422
          Length = 1203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           KTE  +RLQ
Sbjct: 399 KTEECSRLQ 407
>NP_065821
          Length = 1203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           KTE  +RLQ
Sbjct: 399 KTEECSRLQ 407
>NP_001073950
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 306 RTEETKRIQ 314
>NP_665804
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KTESTTRLQ 9
           +TE TTR++
Sbjct: 187 QTEKTTRVR 195
>NP_055309
          Length = 1962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 676 TESTGRLE 683
>XP_006721103
          Length = 1597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 644 TESTGRLE 651
>XP_005255314
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 423 TESTGRLE 430
>XP_006721102
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 534 TESTGRLE 541
>XP_005255313
          Length = 1822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 644 TESTGRLE 651
>XP_005255312
          Length = 1871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 644 TESTGRLE 651
>XP_006721101
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 644 TESTGRLE 651
>XP_005255311
          Length = 1913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 676 TESTGRLE 683
>XP_005255310
          Length = 1919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TESTTRLQ 9
           TEST RL+
Sbjct: 644 TESTGRLE 651
>XP_005267232
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 306 RTEETKRIQ 314
>XP_005267230
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 141 RTEETKRIQ 149
>XP_005267229
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 306 RTEETKRIQ 314
>XP_005267228
          Length = 813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 305 RTEETKRIQ 313
>XP_005267227
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 306 RTEETKRIQ 314
>XP_005267226
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTESTTRLQ 9
           +TE T R+Q
Sbjct: 306 RTEETKRIQ 314
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.103    0.235 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 379,634
Number of extensions: 612
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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