Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     509
XP_006713721                                                           20     509
NP_001193928                                                           17    4312
NP_009105                                                              17    4312
XP_005253886                                                           17    4312
XP_006719269                                                           17    4312
NP_001275544                                                           16    7355
NP_570925                                                              16    7355
NP_570924                                                              16    7355
NP_570923                                                              16    7355
NP_002841                                                              16    7355
NP_001275545                                                           16    7355
XP_006722884                                                           16    7355
XP_005259667                                                           16    7355
XP_005259666                                                           16    7355
XP_006722883                                                           16    7355
XP_006722882                                                           16    7355
XP_006722881                                                           16    7355
XP_005259664                                                           16    7355
XP_005259663                                                           16    7355
XP_006722880                                                           16    7355
XP_006722879                                                           16    7355
XP_006722878                                                           16    7355
XP_006722877                                                           16    7355
XP_006722876                                                           16    7355
XP_006722875                                                           16    7355
XP_005259658                                                           16    7355
XP_005259657                                                           16    7355
XP_006722874                                                           16    7355
XP_006722873                                                           16    7355
XP_006722872                                                           16    7355
XP_006722871                                                           16    7355
XP_006720713                                                           16    7355
XP_006720712                                                           16    7355
XP_006720711                                                           16    7355
XP_005255029                                                           16    7355
XP_005255030                                                           16    7355
XP_006720710                                                           16    7355
XP_005255027                                                           16    7355
XP_005255028                                                           16    7355
XP_005255026                                                           16    7355
NP_001120                                                              15    9605
NP_001796                                                              15    9605
NP_001240813                                                           15    9605
NP_055771                                                              15    9605
NP_001258862                                                           15    9605
NP_056216                                                              15    9605
NP_001091886                                                           15    9605
NP_079420                                                              15    9605
NP_001095876                                                           15    9605
XP_006719521                                                           15    9605
XP_006719520                                                           15    9605
XP_006719519                                                           15    9605
XP_005269025                                                           15    9605
XP_006719518                                                           15    9605
XP_006719517                                                           15    9605
XP_005271517                                                           15    9605
XP_006718857                                                           15    9605
XP_005271516                                                           15    9605
XP_005271515                                                           15    9605
XP_005271514                                                           15    9605
XP_005271513                                                           15    9605
XP_005271512                                                           15    9605
XP_005271511                                                           15    9605
XP_006718856                                                           15    9605
XP_006718855                                                           15    9605
XP_006718854                                                           15    9605
XP_006718853                                                           15    9605
XP_005271510                                                           15    9605
XP_005271509                                                           15    9605
XP_006718852                                                           15    9605
XP_006718851                                                           15    9605
XP_005263305                                                           15    9605
XP_005263304                                                           15    9605
XP_006714389                                                           15    9605
XP_005263303                                                           15    9605
XP_005263302                                                           15    9605
>NP_006209
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTEQAALEY 9
            KTEQ ALEY
Sbjct: 1030 KTEQEALEY 1038
>XP_006713721
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KTEQAALEY 9
            KTEQ ALEY
Sbjct: 1030 KTEQEALEY 1038
>NP_001193928
          Length = 2069

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KTEQAALEY 9
            KTE++ LEY
Sbjct: 1230 KTERSDLEY 1238
>NP_009105
          Length = 2027

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KTEQAALEY 9
            KTE++ LEY
Sbjct: 1188 KTERSDLEY 1196
>XP_005253886
          Length = 1545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KTEQAALEY 9
           KTE++ LEY
Sbjct: 722 KTERSDLEY 730
>XP_006719269
          Length = 2054

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KTEQAALEY 9
            KTE++ LEY
Sbjct: 1230 KTERSDLEY 1238
>NP_001275544
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>NP_570925
          Length = 1505

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1491 QAALEY 1496
>NP_570924
          Length = 1910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1896 QAALEY 1901
>NP_570923
          Length = 1501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1487 QAALEY 1492
>NP_002841
          Length = 1948

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1934 QAALEY 1939
>NP_001275545
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_006722884
          Length = 1490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1476 QAALEY 1481
>XP_005259667
          Length = 1497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1483 QAALEY 1488
>XP_005259666
          Length = 1501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1487 QAALEY 1492
>XP_006722883
          Length = 1510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1496 QAALEY 1501
>XP_006722882
          Length = 1522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1508 QAALEY 1513
>XP_006722881
          Length = 1526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1512 QAALEY 1517
>XP_005259664
          Length = 1906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1892 QAALEY 1897
>XP_005259663
          Length = 1910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1896 QAALEY 1901
>XP_006722880
          Length = 1915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1901 QAALEY 1906
>XP_006722879
          Length = 1917

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1903 QAALEY 1908
>XP_006722878
          Length = 1924

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1910 QAALEY 1915
>XP_006722877
          Length = 1928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1914 QAALEY 1919
>XP_006722876
          Length = 1933

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1919 QAALEY 1924
>XP_006722875
          Length = 1935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1921 QAALEY 1926
>XP_005259658
          Length = 1935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1921 QAALEY 1926
>XP_005259657
          Length = 1935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1921 QAALEY 1926
>XP_006722874
          Length = 1940

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1926 QAALEY 1931
>XP_006722873
          Length = 1944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1930 QAALEY 1935
>XP_006722872
          Length = 1944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1930 QAALEY 1935
>XP_006722871
          Length = 1944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    QAALEY 9
            QAALEY
Sbjct: 1930 QAALEY 1935
>XP_006720713
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 225 QAALEY 230
>XP_006720712
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_006720711
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_005255029
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_005255030
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_006720710
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_005255027
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_005255028
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>XP_005255026
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QAALEY 9
           QAALEY
Sbjct: 210 QAALEY 215
>NP_001120
          Length = 1158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTEQAALEY 9
           +TEQ  L+Y
Sbjct: 227 ETEQPTLDY 235
>NP_001796
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KTEQAALEY 9
           +T+Q  LEY
Sbjct: 231 ETQQKVLEY 239
>NP_001240813
          Length = 1166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1071 KTEYKATEY 1079
>NP_055771
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 734 KSEQAAL 740
>NP_001258862
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 737 KSEQAAL 743
>NP_056216
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 134 KKQQAALE 141
>NP_001091886
          Length = 954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 134 KKQQAALE 141
>NP_079420
          Length = 1244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1149 KTEYKATEY 1157
>NP_001095876
          Length = 1244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1149 KTEYKATEY 1157
>XP_006719521
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 148 KKQQAALE 155
>XP_006719520
          Length = 950

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 117 KKQQAALE 124
>XP_006719519
          Length = 968

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 148 KKQQAALE 155
>XP_005269025
          Length = 974

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 117 KKQQAALE 124
>XP_006719518
          Length = 981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 148 KKQQAALE 155
>XP_006719517
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KTEQAALE 8
           K +QAALE
Sbjct: 148 KKQQAALE 155
>XP_005271517
          Length = 1350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 624 KSEQAAL 630
>XP_006718857
          Length = 1434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 708 KSEQAAL 714
>XP_005271516
          Length = 1437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 711 KSEQAAL 717
>XP_005271515
          Length = 1437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 711 KSEQAAL 717
>XP_005271514
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 737 KSEQAAL 743
>XP_005271513
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 734 KSEQAAL 740
>XP_005271512
          Length = 1463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 737 KSEQAAL 743
>XP_005271511
          Length = 1463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KTEQAAL 7
           K+EQAAL
Sbjct: 737 KSEQAAL 743
>XP_006718856
          Length = 1976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1250 KSEQAAL 1256
>XP_006718855
          Length = 1979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1253 KSEQAAL 1259
>XP_006718854
          Length = 1999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1273 KSEQAAL 1279
>XP_006718853
          Length = 2017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1299 KSEQAAL 1305
>XP_005271510
          Length = 2022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1296 KSEQAAL 1302
>XP_005271509
          Length = 2024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1299 KSEQAAL 1305
>XP_006718852
          Length = 2025

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1299 KSEQAAL 1305
>XP_006718851
          Length = 2025

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KTEQAAL 7
            K+EQAAL
Sbjct: 1299 KSEQAAL 1305
>XP_005263305
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1071 KTEYKATEY 1079
>XP_005263304
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1071 KTEYKATEY 1079
>XP_006714389
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1129 KTEYKATEY 1137
>XP_005263303
          Length = 1245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1149 KTEYKATEY 1157
>XP_005263302
          Length = 1245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    KTEQAALEY 9
            KTE  A EY
Sbjct: 1149 KTEYKATEY 1157
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.111    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,450
Number of extensions: 695
Number of successful extensions: 77
Number of sequences better than 10000.0: 77
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 77
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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