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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_005568                                                              15    9605
NP_066015                                                              15    9605
NP_997248                                                              15    9605
XP_005258381                                                           15    9605
XP_006722580                                                           15    9605
XP_006722579                                                           15    9605
XP_005258380                                                           15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KSTAVSQQI 9
           KST VSQQI
Sbjct: 239 KSTTVSQQI 247



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  KSTAVSQQI 9
          KST VSQQI
Sbjct: 34 KSTTVSQQI 42



>NP_005568
          Length = 2040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    STAVSQQ 8
            STAVS+Q
Sbjct: 1256 STAVSEQ 1262



>NP_066015
          Length = 2579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KSTAVSQQI 9
            K+ A+SQQ+
Sbjct: 1609 KNEAISQQL 1617



>NP_997248
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   STAVSQQI 9
           +TAV+QQ+
Sbjct: 195 ATAVTQQV 202



>XP_005258381
          Length = 2224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KSTAVSQQI 9
            K+ A+SQQ+
Sbjct: 1254 KNEAISQQL 1262



>XP_006722580
          Length = 2532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KSTAVSQQI 9
            K+ A+SQQ+
Sbjct: 1562 KNEAISQQL 1570



>XP_006722579
          Length = 2544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KSTAVSQQI 9
            K+ A+SQQ+
Sbjct: 1609 KNEAISQQL 1617



>XP_005258380
          Length = 2578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KSTAVSQQI 9
            K+ A+SQQ+
Sbjct: 1609 KNEAISQQL 1617



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.101    0.215 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 229,619
Number of extensions: 158
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)