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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     299
XP_005262424                                                           20     299
NP_001010872                                                           17    3301
XP_006715085                                                           17    3301
XP_005270830                                                           17    4312
NP_001035799                                                           17    4312
NP_963844                                                              16    5631
NP_057413                                                              16    7355
NP_001254645                                                           16    7355
NP_001265662                                                           16    7355
NP_473455                                                              16    7355
NP_687034                                                              16    7355
XP_005276374                                                           16    7355
XP_006722813                                                           16    7355
XP_005273748                                                           16    7355
XP_006713267                                                           16    7355
XP_005265278                                                           16    7355
XP_006713266                                                           16    7355
XP_005276074                                                           16    7355
NP_036313                                                              15    9605
NP_001697                                                              15    9605
NP_001124317                                                           15    9605
NP_001129082                                                           15    9605
NP_001258698                                                           15    9605
NP_003655                                                              15    9605
XP_005259899                                                           15    9605
XP_005259898                                                           15    9605
XP_005247751                                                           15    9605

>NP_722576
          Length = 3080

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KSSKTMHAG 9
            KSSKTMH G
Sbjct: 1538 KSSKTMHPG 1546



>XP_005262424
          Length = 2875

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KSSKTMHAG 9
            KSSKTMH G
Sbjct: 1333 KSSKTMHPG 1341



>NP_001010872
          Length = 1011

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KSSKTMH 7
           KSSK+MH
Sbjct: 705 KSSKSMH 711



>XP_006715085
          Length = 1011

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KSSKTMH 7
           KSSK+MH
Sbjct: 705 KSSKSMH 711



>XP_005270830
          Length = 236

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSKTMH 7
           SSKTMH
Sbjct: 101 SSKTMH 106



>NP_001035799
          Length = 272

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SSKTMH 7
           SSKTMH
Sbjct: 101 SSKTMH 106



>NP_963844
          Length = 277

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTMHAG 9
           KT+HAG
Sbjct: 147 KTLHAG 152



>NP_057413
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSKTMHAG 9
           SS+T+H G
Sbjct: 475 SSRTVHEG 482



>NP_001254645
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTMHAG 9
           KT+HAG
Sbjct: 303 KTIHAG 308



>NP_001265662
          Length = 668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KSSKTMHA 8
           K S+T+HA
Sbjct: 605 KQSRTVHA 612



>NP_473455
          Length = 731

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KSSKTMHA 8
           K S+T+HA
Sbjct: 668 KQSRTVHA 675



>NP_687034
          Length = 553

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KSSKTMHA 8
           K S+T+HA
Sbjct: 490 KQSRTVHA 497



>XP_005276374
          Length = 264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SSKTMHA 8
          S+KT+HA
Sbjct: 27 SAKTLHA 33



>XP_006722813
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTMHAG 9
           KT+HAG
Sbjct: 303 KTIHAG 308



>XP_005273748
          Length = 264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SSKTMHA 8
          S+KT+HA
Sbjct: 27 SAKTLHA 33



>XP_006713267
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSKTMHAG 9
           SS+T+H G
Sbjct: 475 SSRTVHEG 482



>XP_005265278
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSKTMHAG 9
           SS+T+H G
Sbjct: 475 SSRTVHEG 482



>XP_006713266
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSKTMHAG 9
           SS+T+H G
Sbjct: 475 SSRTVHEG 482



>XP_005276074
          Length = 264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SSKTMHA 8
          S+KT+HA
Sbjct: 27 SAKTLHA 33



>NP_036313
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SSKTMHA 8
           S+KT+HA
Sbjct: 339 SNKTIHA 345



>NP_001697
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SSKTMHAG 9
           S KT+H G
Sbjct: 563 SHKTVHTG 570



>NP_001124317
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SSKTMHAG 9
           S KT+H G
Sbjct: 563 SHKTVHTG 570



>NP_001129082
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSKTMHAG 9
           SSK+ H+G
Sbjct: 207 SSKSRHSG 214



>NP_001258698
          Length = 1045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    SSKTMHAG 9
            ++KT+H+G
Sbjct: 997  AAKTVHSG 1004



>NP_003655
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    SSKTMHAG 9
            ++KT+H+G
Sbjct: 1046 AAKTVHSG 1053



>XP_005259899
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SSKTMHA 8
           S+KT+HA
Sbjct: 367 SNKTIHA 373



>XP_005259898
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SSKTMHA 8
           S+KT+HA
Sbjct: 368 SNKTIHA 374



>XP_005247751
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SSKTMHAG 9
           S KT+H G
Sbjct: 563 SHKTVHTG 570



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.104    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 411,532
Number of extensions: 420
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)