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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     509
NP_858045                                                              20     509
NP_006525                                                              20     509
NP_001167559                                                           20     509
NP_919263                                                              18    1482
XP_006720118                                                           18    1482
XP_006720117                                                           18    1482
XP_006720116                                                           18    1482
XP_005267417                                                           18    1482
NP_112174                                                              17    3301
NP_003292                                                              16    5631
NP_060708                                                              16    7355
XP_005250080                                                           16    7355
XP_005250078                                                           16    7355
NP_001018098                                                           15    9605
NP_060154                                                              15    9605
NP_060550                                                              15    9605
NP_705835                                                              15    9605
NP_065872                                                              15    9605
NP_060897                                                              15    9605
NP_001265506                                                           15    9605
NP_001265507                                                           15    9605
NP_001265505                                                           15    9605
NP_001265504                                                           15    9605
NP_001161866                                                           15    9605
NP_001161865                                                           15    9605
NP_001192202                                                           15    9605
XP_006725262                                                           15    9605
XP_006725261                                                           15    9605
XP_006725260                                                           15    9605
XP_005255434                                                           15    9605
XP_005255433                                                           15    9605
XP_005255432                                                           15    9605
XP_005265878                                                           15    9605
XP_006714885                                                           15    9605

>NP_001167558
          Length = 1423

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           KSKLF NPV
Sbjct: 348 KSKLFRNPV 356



>NP_858045
          Length = 1424

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           KSKLF NPV
Sbjct: 348 KSKLFRNPV 356



>NP_006525
          Length = 1420

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           KSKLF NPV
Sbjct: 348 KSKLFRNPV 356



>NP_001167559
          Length = 1415

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           KSKLF NPV
Sbjct: 358 KSKLFRNPV 366



>NP_919263
          Length = 488

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLFLNPV 9
           KLFLNP+
Sbjct: 336 KLFLNPI 342



>XP_006720118
          Length = 248

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLFLNPV 9
           KLFLNP+
Sbjct: 96  KLFLNPI 102



>XP_006720117
          Length = 396

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLFLNPV 9
           KLFLNP+
Sbjct: 244 KLFLNPI 250



>XP_006720116
          Length = 396

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLFLNPV 9
           KLFLNP+
Sbjct: 244 KLFLNPI 250



>XP_005267417
          Length = 471

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLFLNPV 9
           KLFLNP+
Sbjct: 319 KLFLNPI 325



>NP_112174
          Length = 551

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SKLFLNPV 9
           SKLFLN V
Sbjct: 330 SKLFLNEV 337



>NP_003292
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LFLNPV 9
           LFLNP+
Sbjct: 218 LFLNPI 223



>NP_060708
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  KSKLFLNP 8
          K+ +FLNP
Sbjct: 62 KATVFLNP 69



>XP_005250080
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  KSKLFLNP 8
          K+ +FLNP
Sbjct: 62 KATVFLNP 69



>XP_005250078
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  KSKLFLNP 8
          K+ +FLNP
Sbjct: 62 KATVFLNP 69



>NP_001018098
          Length = 3628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SKLFLNP 8
           +KL++NP
Sbjct: 303 AKLYMNP 309



>NP_060154
          Length = 3710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SKLFLNP 8
           +KL++NP
Sbjct: 260 AKLYMNP 266



>NP_060550
          Length = 3585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SKLFLNP 8
           +KL++NP
Sbjct: 260 AKLYMNP 266



>NP_705835
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           K+K+ +NPV
Sbjct: 237 KAKVKINPV 245



>NP_065872
          Length = 3753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SKLFLNP 8
           +KL++NP
Sbjct: 303 AKLYMNP 309



>NP_060897
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 569 RSKKFIDPI 577



>NP_001265506
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSKLFLNPV 9
           K+KLFL  V
Sbjct: 106 KAKLFLKEV 114



>NP_001265507
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSKLFLNPV 9
           K+KLFL  V
Sbjct: 106 KAKLFLKEV 114



>NP_001265505
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSKLFLNPV 9
           K+KLFL  V
Sbjct: 106 KAKLFLKEV 114



>NP_001265504
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSKLFLNPV 9
           K+KLFL  V
Sbjct: 106 KAKLFLKEV 114



>NP_001161866
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           K+K+ +NPV
Sbjct: 237 KAKVKINPV 245



>NP_001161865
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           K+K+ +NPV
Sbjct: 237 KAKVKINPV 245



>NP_001192202
          Length = 148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSKLFLNPV 9
           K+KLFL  V
Sbjct: 114 KAKLFLKEV 122



>XP_006725262
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 357 RSKKFIDPI 365



>XP_006725261
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 357 RSKKFIDPI 365



>XP_006725260
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 539 RSKKFIDPI 547



>XP_005255434
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 357 RSKKFIDPI 365



>XP_005255433
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 357 RSKKFIDPI 365



>XP_005255432
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           +SK F++P+
Sbjct: 539 RSKKFIDPI 547



>XP_005265878
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           K+K+ +NPV
Sbjct: 229 KAKVKINPV 237



>XP_006714885
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSKLFLNPV 9
           K+K+ +NPV
Sbjct: 237 KAKVKINPV 245



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.139    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 670,038
Number of extensions: 1220
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)