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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     509
XP_005251512                                                           20     509
NP_002218                                                              18    1482
XP_005270898                                                           18    1482
XP_006710687                                                           18    1482
NP_000531                                                              17    3301
NP_001036188                                                           17    3301
NP_001271260                                                           17    3301
NP_112598                                                              17    3301
XP_006723382                                                           17    3301
XP_006723381                                                           17    3301
XP_006723380                                                           17    3301
NP_037416                                                              17    3301
NP_872353                                                              17    4312
NP_059980                                                              17    4312
NP_055230                                                              17    4312
NP_077305                                                              16    5631
NP_001278220                                                           16    5631
NP_000206                                                              16    5631
XP_006722808                                                           16    5631
XP_005259953                                                           16    5631
NP_003549                                                              16    7355
NP_001265182                                                           16    7355
NP_037428                                                              16    7355
XP_006717364                                                           16    7355
XP_005252318                                                           16    7355
XP_006717363                                                           16    7355
XP_005252319                                                           16    7355
XP_006710652                                                           16    7355
XP_006710651                                                           16    7355
XP_005270844                                                           16    7355
NP_116741                                                              15    9605
NP_060698                                                              15    9605

>NP_004963
          Length = 1132

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KSFFEAASM 9
           +SFFEAASM
Sbjct: 592 ESFFEAASM 600



>XP_005251512
          Length = 1132

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KSFFEAASM 9
           +SFFEAASM
Sbjct: 592 ESFFEAASM 600



>NP_002218
          Length = 1154

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SFFEAASM 9
           +FFEAASM
Sbjct: 634 AFFEAASM 641



>XP_005270898
          Length = 1154

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SFFEAASM 9
           +FFEAASM
Sbjct: 634 AFFEAASM 641



>XP_006710687
          Length = 1154

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SFFEAASM 9
           +FFEAASM
Sbjct: 634 AFFEAASM 641



>NP_000531
          Length = 5038

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KSFFEAAS 8
            +SFFE+AS
Sbjct: 3093 RSFFESAS 3100



>NP_001036188
          Length = 5033

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KSFFEAAS 8
            +SFFE+AS
Sbjct: 3093 RSFFESAS 3100



>NP_001271260
          Length = 457

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSFFEAAS 8
           K+FF+AAS
Sbjct: 430 KTFFQAAS 437



>NP_112598
          Length = 5088

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSFFEAAS 8
           KSFF+AA+
Sbjct: 299 KSFFQAAT 306



>XP_006723382
          Length = 5027

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KSFFEAAS 8
            +SFFE+AS
Sbjct: 3093 RSFFESAS 3100



>XP_006723381
          Length = 5031

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KSFFEAAS 8
            +SFFE+AS
Sbjct: 3092 RSFFESAS 3099



>XP_006723380
          Length = 5032

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KSFFEAAS 8
            +SFFE+AS
Sbjct: 3093 RSFFESAS 3100



>NP_037416
          Length = 494

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSFFEAAS 8
           K+FF+AAS
Sbjct: 467 KTFFQAAS 474



>NP_872353
          Length = 227

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSFFEAASM 9
           KSFFEA  +
Sbjct: 218 KSFFEAKKL 226



>NP_059980
          Length = 235

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSFFEAASM 9
           KSFFEA  +
Sbjct: 226 KSFFEAKKL 234



>NP_055230
          Length = 1053

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSFFEAASM 9
           KSFFEA  +
Sbjct: 773 KSFFEARDL 781



>NP_077305
          Length = 240

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KSFFEA 6
           KSFFEA
Sbjct: 191 KSFFEA 196



>NP_001278220
          Length = 219

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KSFFEAAS 8
           K FFEAA+
Sbjct: 100 KRFFEAAA 107



>NP_000206
          Length = 1124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSFFEAASM 9
           +SF EAAS+
Sbjct: 567 ESFLEAASL 575



>XP_006722808
          Length = 1124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSFFEAASM 9
           +SF EAAS+
Sbjct: 567 ESFLEAASL 575



>XP_005259953
          Length = 1167

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSFFEAASM 9
           +SF EAAS+
Sbjct: 610 ESFLEAASL 618



>NP_003549
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFFEAASM 9
           S FEAAS+
Sbjct: 468 SLFEAASL 475



>NP_001265182
          Length = 502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFFEAASM 9
           S FEAAS+
Sbjct: 468 SLFEAASL 475



>NP_037428
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SFFEAA 7
          SFFEAA
Sbjct: 45 SFFEAA 50



>XP_006717364
          Length = 502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFFEAASM 9
           S FEAAS+
Sbjct: 468 SLFEAASL 475



>XP_005252318
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFFEAASM 9
           S FEAAS+
Sbjct: 468 SLFEAASL 475



>XP_006717363
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFFEAASM 9
           S FEAAS+
Sbjct: 468 SLFEAASL 475



>XP_005252319
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SFFEAASM 9
           S FEAAS+
Sbjct: 468 SLFEAASL 475



>XP_006710652
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SFFEAA 7
          SFFEAA
Sbjct: 26 SFFEAA 31



>XP_006710651
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SFFEAA 7
          SFFEAA
Sbjct: 45 SFFEAA 50



>XP_005270844
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SFFEAA 7
          SFFEAA
Sbjct: 45 SFFEAA 50



>NP_116741
          Length = 113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  KSFFEAAS 8
          KS+FE +S
Sbjct: 68 KSYFETSS 75



>NP_060698
          Length = 702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  FEAASM 9
          FEAASM
Sbjct: 87 FEAASM 92



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.119    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,429
Number of extensions: 329
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)