Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_056150                                                              17    3301
XP_006719370                                                           17    3301
XP_006719369                                                           17    3301
XP_006719368                                                           17    3301
NP_001034230                                                           17    4312
NP_036336                                                              17    4312
NP_000324                                                              16    5631
NP_057532                                                              16    5631
NP_060762                                                              16    5631
NP_001273158                                                           16    5631
NP_001273159                                                           16    5631
NP_001273494                                                           16    5631
NP_001273495                                                           16    5631
NP_055547                                                              16    5631
NP_001121621                                                           16    5631
NP_001170858                                                           16    5631
NP_001182408                                                           16    5631
NP_006061                                                              16    5631
NP_001007566                                                           16    5631
NP_001182407                                                           16    5631
XP_005256324                                                           16    5631
XP_005256322                                                           16    5631
XP_005247123                                                           16    5631
XP_006713536                                                           16    5631
XP_006713535                                                           16    5631
NP_001180270                                                           16    7355
NP_079355                                                              16    7355
NP_689945                                                              16    7355
NP_001273720                                                           16    7355
NP_079363                                                              16    7355
XP_005248958                                                           16    7355
XP_006714093                                                           16    7355
XP_005248183                                                           16    7355
XP_006710988                                                           16    7355
XP_005271284                                                           16    7355
XP_005271283                                                           16    7355
NP_001035987                                                           15    9605
NP_008944                                                              15    9605
NP_055585                                                              15    9605
NP_001116244                                                           15    9605
NP_037534                                                              15    9605
XP_006723282                                                           15    9605
XP_005268683                                                           15    9605
XP_005268682                                                           15    9605
XP_005250818                                                           15    9605
XP_005250817                                                           15    9605
>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KSAFGPTKN 9
            KSA GPTKN
Sbjct: 1775 KSASGPTKN 1783
>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KSAFGPTKN 9
            KSA GPTKN
Sbjct: 1570 KSASGPTKN 1578
>NP_056150
          Length = 2210

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAFGPTKN 9
            SAF PT N
Sbjct: 1560 SAFNPTSN 1567
>XP_006719370
          Length = 2076

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAFGPTKN 9
            SAF PT N
Sbjct: 1426 SAFNPTSN 1433
>XP_006719369
          Length = 2200

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAFGPTKN 9
            SAF PT N
Sbjct: 1550 SAFNPTSN 1557
>XP_006719368
          Length = 2209

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAFGPTKN 9
            SAF PT N
Sbjct: 1559 SAFNPTSN 1566
>NP_001034230
          Length = 2365

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KSAFGPTK 8
           +S+FGPT+
Sbjct: 962 QSSFGPTQ 969
>NP_036336
          Length = 822

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SAFGPTK 8
           SAFGP K
Sbjct: 335 SAFGPIK 341
>NP_000324
          Length = 892

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KSAFGPT 7
           KSA GPT
Sbjct: 271 KSAVGPT 277
>NP_057532
          Length = 730

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KSAFGPTKN 9
           K + GPTK+
Sbjct: 600 KDSLGPTKS 608
>NP_060762
          Length = 528

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 135 FGPVKN 140
>NP_001273158
          Length = 459

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KSAFGPTKN 9
           K + GPTK+
Sbjct: 329 KDSLGPTKS 337
>NP_001273159
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KSAFGPTKN 9
           K + GPTK+
Sbjct: 558 KDSLGPTKS 566
>NP_001273494
          Length = 411

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 175 FGPAKN 180
>NP_001273495
          Length = 401

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 175 FGPAKN 180
>NP_055547
          Length = 411

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 175 FGPAKN 180
>NP_001121621
          Length = 747

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KSAFGPT 7
           KSA GPT
Sbjct: 126 KSAVGPT 132
>NP_001170858
          Length = 945

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KSAFGPT 7
           KSA GPT
Sbjct: 271 KSAVGPT 277
>NP_001182408
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>NP_006061
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>NP_001007566
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>NP_001182407
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>XP_005256324
          Length = 301

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 175 FGPAKN 180
>XP_005256322
          Length = 411

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 175 FGPAKN 180
>XP_005247123
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>XP_006713536
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>XP_006713535
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           SAF P KN
Sbjct: 166 SAFDPLKN 173
>NP_001180270
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KSAFGPT 7
           K+ FGPT
Sbjct: 146 KTPFGPT 152
>NP_079355
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           S+FGPT
Sbjct: 413 SSFGPT 418
>NP_689945
          Length = 276

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KSAFGPT 7
           K+ FGPT
Sbjct: 161 KTPFGPT 167
>NP_001273720
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 339 FGPKKN 344
>NP_079363
          Length = 699

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 366 FGPKKN 371
>XP_005248958
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KSAFGPT 7
           K+ FGPT
Sbjct: 161 KTPFGPT 167
>XP_006714093
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 178 FGPKKN 183
>XP_005248183
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FGPTKN 9
           FGP KN
Sbjct: 280 FGPKKN 285
>XP_006710988
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           S+FGPT
Sbjct: 236 SSFGPT 241
>XP_005271284
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           S+FGPT
Sbjct: 294 SSFGPT 299
>XP_005271283
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           S+FGPT
Sbjct: 413 SSFGPT 418
>NP_001035987
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   SAFGPT 7
           S FGPT
Sbjct: 173 SGFGPT 178
>NP_008944
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           S+F PT N
Sbjct: 247 SSFAPTDN 254
>NP_055585
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           SAFGP+
Sbjct: 409 SAFGPS 414
>NP_001116244
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           SAFGP+
Sbjct: 745 SAFGPS 750
>NP_037534
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AFGPTKN 9
           +F PTKN
Sbjct: 156 SFEPTKN 162
>XP_006723282
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   SAFGPT 7
           S FGPT
Sbjct: 173 SGFGPT 178
>XP_005268683
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           SAFGP+
Sbjct: 409 SAFGPS 414
>XP_005268682
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SAFGPT 7
           SAFGP+
Sbjct: 745 SAFGPS 750
>XP_005250818
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           S+F PT N
Sbjct: 246 SSFAPTDN 253
>XP_005250817
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SAFGPTKN 9
           S+F PT N
Sbjct: 247 SSFAPTDN 254
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.122    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 844,499
Number of extensions: 994
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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