Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006505                                                              16    5631
XP_005265428                                                           16    5631
NP_056180                                                              16    7355
NP_001186069                                                           16    7355
NP_001165160                                                           16    7355
XP_006712850                                                           16    7355
XP_005263865                                                           16    7355
XP_005263864                                                           16    7355
XP_005263863                                                           16    7355
XP_005263861                                                           16    7355
NP_001020751                                                           16    7355
NP_113633                                                              16    7355
NP_005793                                                              15    9605
NP_001182551                                                           15    9605
NP_001124162                                                           15    9605
NP_055655                                                              15    9605
NP_055987                                                              15    9605
XP_006715384                                                           15    9605
XP_005245087                                                           15    9605
XP_005245086                                                           15    9605
XP_005245085                                                           15    9605
XP_005245084                                                           15    9605
XP_006711302                                                           15    9605
XP_005245083                                                           15    9605
XP_005245082                                                           15    9605
XP_005245081                                                           15    9605
XP_005245080                                                           15    9605
XP_005245079                                                           15    9605
XP_005245077                                                           15    9605
XP_005245076                                                           15    9605
XP_006711301                                                           15    9605
XP_005245075                                                           15    9605
XP_005245074                                                           15    9605
XP_005245073                                                           15    9605
XP_005245072                                                           15    9605
>NP_006505
          Length = 1956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K+T  SAEY
Sbjct: 592 KKTFLSAEY 600
>XP_005265428
          Length = 1959

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K+T  SAEY
Sbjct: 595 KKTFLSAEY 603
>NP_056180
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K T+S AEY
Sbjct: 301 KMTLSYAEY 309
>NP_001186069
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 279 KVTISYAEY 287
>NP_001165160
          Length = 210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K T+S AEY
Sbjct: 178 KMTLSYAEY 186
>XP_006712850
          Length = 196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 165 KVTISYAEY 173
>XP_005263865
          Length = 196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 165 KVTISYAEY 173
>XP_005263864
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 242 KVTISYAEY 250
>XP_005263863
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 287 KVTISYAEY 295
>XP_005263861
          Length = 355

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 324 KVTISYAEY 332
>NP_001020751
          Length = 296

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K T+S AEY
Sbjct: 264 KMTLSYAEY 272
>NP_113633
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           K TIS AEY
Sbjct: 279 KVTISYAEY 287
>NP_005793
          Length = 1045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           +RT+S A Y
Sbjct: 722 RRTLSRAHY 730
>NP_001182551
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KRTISSAEY 9
           +RT+S A Y
Sbjct: 657 RRTLSRAHY 665
>NP_001124162
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TISSAEY 9
           T+SS+EY
Sbjct: 613 TLSSSEY 619
>NP_055655
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RTISSAEY 9
           R ISS EY
Sbjct: 263 RQISSGEY 270
>NP_055987
          Length = 2817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2173 SVSSAEY 2179
>XP_006715384
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TISSAEY 9
           T+SS+EY
Sbjct: 596 TLSSSEY 602
>XP_005245087
          Length = 2708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245086
          Length = 2714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245085
          Length = 2718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245084
          Length = 2719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_006711302
          Length = 2754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245083
          Length = 2774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245082
          Length = 2779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245081
          Length = 2819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245080
          Length = 2824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245079
          Length = 2838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245077
          Length = 2858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245076
          Length = 2869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_006711301
          Length = 2896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2173 SVSSAEY 2179
>XP_005245075
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2170 SVSSAEY 2176
>XP_005245074
          Length = 2898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
>XP_005245073
          Length = 2901

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2173 SVSSAEY 2179
>XP_005245072
          Length = 2903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TISSAEY 9
            ++SSAEY
Sbjct: 2175 SVSSAEY 2181
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.111    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 324,995
Number of extensions: 456
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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