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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001244954                                                           17    4312
NP_689833                                                              17    4312
NP_001243089                                                           17    4312
NP_079273                                                              17    4312
NP_659410                                                              17    4312
XP_006721017                                                           17    4312
XP_006721016                                                           17    4312
XP_005253387                                                           17    4312
XP_006719103                                                           17    4312
XP_005253384                                                           17    4312
XP_005253383                                                           17    4312
NP_001189394                                                           16    7355
NP_001189393                                                           16    7355
NP_006615                                                              16    7355
NP_001189395                                                           16    7355
NP_001012425                                                           16    7355
NP_003366                                                              16    7355
NP_001189396                                                           16    7355
NP_997644                                                              16    7355
NP_001189397                                                           16    7355
NP_001171752                                                           16    7355
NP_001171712                                                           16    7355
XP_006718024                                                           16    7355
XP_005252420                                                           16    7355
XP_006717439                                                           16    7355
XP_005252419                                                           16    7355
XP_005252418                                                           16    7355
XP_005252417                                                           16    7355
XP_005252416                                                           16    7355
NP_001104740                                                           15    9605
NP_891992                                                              15    9605
NP_006773                                                              15    9605

>NP_001244954
          Length = 471

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  KRQAVNMMC 9
          KRQ  N MC
Sbjct: 89 KRQKENAMC 97



>NP_689833
          Length = 471

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  KRQAVNMMC 9
          KRQ  N MC
Sbjct: 89 KRQKENAMC 97



>NP_001243089
          Length = 543

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KRQAVNMMC 9
           KRQ+ N++C
Sbjct: 212 KRQSDNILC 220



>NP_079273
          Length = 682

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KRQAVNMMC 9
           KRQ+ N++C
Sbjct: 212 KRQSDNILC 220



>NP_659410
          Length = 1274

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RQAVNMMC 9
          + AVNM+C
Sbjct: 74 QDAVNMLC 81



>XP_006721017
          Length = 366

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KRQAVNMMC 9
           KRQ+ N++C
Sbjct: 212 KRQSDNILC 220



>XP_006721016
          Length = 682

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KRQAVNMMC 9
           KRQ+ N++C
Sbjct: 212 KRQSDNILC 220



>XP_005253387
          Length = 1156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQAVNMMC 9
           + AVNM+C
Sbjct: 96  QDAVNMLC 103



>XP_006719103
          Length = 1296

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQAVNMMC 9
           + AVNM+C
Sbjct: 96  QDAVNMLC 103



>XP_005253384
          Length = 1311

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RQAVNMMC 9
          + AVNM+C
Sbjct: 74 QDAVNMLC 81



>XP_005253383
          Length = 1333

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQAVNMMC 9
           + AVNM+C
Sbjct: 96  QDAVNMLC 103



>NP_001189394
          Length = 517

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 424 KRQAVN 429



>NP_001189393
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 455 KRQAVN 460



>NP_006615
          Length = 602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 509 KRQAVN 514



>NP_001189395
          Length = 547

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 454 KRQAVN 459



>NP_001012425
          Length = 180

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   AVNMMC 9
           A+N+MC
Sbjct: 127 AINLMC 132



>NP_003366
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KRQAVNMMC 9
           KR+ +N+ C
Sbjct: 130 KRECINLGC 138



>NP_001189396
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 455 KRQAVN 460



>NP_997644
          Length = 567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 508 KRQAVN 513



>NP_001189397
          Length = 568

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 509 KRQAVN 514



>NP_001171752
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KRQAVNMMC 9
           KR+ +N+ C
Sbjct: 130 KRECINLGC 138



>NP_001171712
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KRQAVNMMC 9
           KR+ +N+ C
Sbjct: 145 KRECINLGC 153



>XP_006718024
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KRQAVNMMC 9
           KR+ +N+ C
Sbjct: 130 KRECINLGC 138



>XP_005252420
          Length = 547

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 454 KRQAVN 459



>XP_006717439
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 455 KRQAVN 460



>XP_005252419
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 455 KRQAVN 460



>XP_005252418
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 455 KRQAVN 460



>XP_005252417
          Length = 601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 508 KRQAVN 513



>XP_005252416
          Length = 602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KRQAVN 6
           KRQAVN
Sbjct: 509 KRQAVN 514



>NP_001104740
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QAVNMMC 9
           QAV +MC
Sbjct: 360 QAVEVMC 366



>NP_891992
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QAVNMMC 9
           QAV +MC
Sbjct: 321 QAVEVMC 327



>NP_006773
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1  KRQAVNMMC 9
          KR+  N+ C
Sbjct: 8  KRKVTNLFC 16



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.125    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,433
Number of extensions: 444
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)