Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005036                                                              17    4312
NP_774959                                                              17    4312
NP_001254711                                                           16    7355
NP_001254712                                                           16    7355
NP_060219                                                              16    7355
XP_006718323                                                           16    7355
XP_005253071                                                           16    7355
XP_005253069                                                           16    7355
XP_005253068                                                           16    7355
XP_006718322                                                           16    7355
XP_005253066                                                           16    7355
NP_059138                                                              15    9605
NP_056262                                                              15    9605
NP_001034                                                              15    9605
NP_001165972                                                           15    9605
NP_001165973                                                           15    9605
NP_001137505                                                           15    9605
NP_001165975                                                           15    9605
XP_006721326                                                           15    9605
XP_005266331                                                           15    9605
XP_005263454                                                           15    9605
XP_005263453                                                           15    9605
NP_660329                                                              15    9605
>NP_005036
          Length = 3460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  RATFLLAL 9
          R TFLLAL
Sbjct: 8  RQTFLLAL 15
>NP_774959
          Length = 3458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  RATFLLAL 9
          R TFLLAL
Sbjct: 8  RQTFLLAL 15
>NP_001254711
          Length = 1301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>NP_001254712
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>NP_060219
          Length = 1208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>XP_006718323
          Length = 1119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>XP_005253071
          Length = 1208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>XP_005253069
          Length = 1238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>XP_005253068
          Length = 1269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>XP_006718322
          Length = 1298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>XP_005253066
          Length = 1298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          R+TFLLA
Sbjct: 54 RSTFLLA 60
>NP_059138
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RATFLLA 8
           RA+FLLA
Sbjct: 948 RASFLLA 954
>NP_056262
          Length = 1602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    KRATFLL 7
            KRA FLL
Sbjct: 1286 KRAAFLL 1292
>NP_001034
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ATFLLAL 9
           +TFLLAL
Sbjct: 452 STFLLAL 458
>NP_001165972
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ATFLLAL 9
           +TFLLAL
Sbjct: 452 STFLLAL 458
>NP_001165973
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ATFLLAL 9
           +TFLLAL
Sbjct: 347 STFLLAL 353
>NP_001137505
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RATFLLA 8
           +ATFLLA
Sbjct: 129 QATFLLA 135
>NP_001165975
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ATFLLAL 9
           +TFLLAL
Sbjct: 452 STFLLAL 458
>XP_006721326
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ATFLLAL 9
           +TFLLAL
Sbjct: 452 STFLLAL 458
>XP_005266331
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RATFLLA 8
          +ATFLLA
Sbjct: 88 QATFLLA 94
>XP_005263454
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    KRATFLL 7
            KRA FLL
Sbjct: 1008 KRAAFLL 1014
>XP_005263453
          Length = 1613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    KRATFLL 7
            KRA FLL
Sbjct: 1297 KRAAFLL 1303
>NP_660329
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RATFLLA 8
           +ATFLLA
Sbjct: 138 QATFLLA 144
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.146    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 294,521
Number of extensions: 261
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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