Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     509
XP_006716142                                                           20     509
XP_005250088                                                           20     509
XP_006716141                                                           20     509
XP_005250085                                                           20     509
XP_006716140                                                           20     509
XP_005250084                                                           20     509
XP_005250083                                                           20     509
XP_005250082                                                           20     509
NP_001714                                                              18    1935
XP_005249383                                                           18    1935
NP_001255981                                                           17    3301
NP_001255980                                                           17    3301
NP_665803                                                              17    3301
NP_001166094                                                           16    5631
NP_002490                                                              16    5631
XP_005254473                                                           16    5631
XP_005254472                                                           16    5631
XP_005254471                                                           16    5631
XP_005254470                                                           16    5631
XP_005254469                                                           16    5631
XP_005254468                                                           16    5631
XP_005254467                                                           16    5631
XP_005254465                                                           16    5631
NP_001166095                                                           16    5631
NP_068781                                                              16    7355
NP_065101                                                              16    7355
NP_001153218                                                           16    7355
NP_057636                                                              16    7355
NP_001269710                                                           16    7355
NP_001269709                                                           16    7355
NP_001269700                                                           16    7355
NP_001243776                                                           16    7355
NP_001269078                                                           16    7355
NP_001083                                                              16    7355
XP_006725355                                                           16    7355
XP_006721570                                                           16    7355
XP_006712635                                                           16    7355
XP_006712634                                                           16    7355
XP_006712633                                                           16    7355
XP_006712632                                                           16    7355
XP_005247077                                                           16    7355
XP_006712631                                                           16    7355
XP_006712630                                                           16    7355
NP_078853                                                              15    9605
NP_001018082                                                           15    9605
NP_001017523                                                           15    9605
NP_001193762                                                           15    9605
NP_874369                                                              15    9605
NP_006379                                                              15    9605
NP_874368                                                              15    9605
NP_057363                                                              15    9605
XP_005268702                                                           15    9605
XP_006718484                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3875 KQAMYSSTD 3883
>XP_006716142
          Length = 4927

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>XP_005250088
          Length = 4928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>XP_006716141
          Length = 4959

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>XP_005250085
          Length = 4979

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>XP_006716140
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>XP_005250084
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>XP_005250083
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3891 KQAMYSSTD 3899
>XP_005250082
          Length = 4983

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KQAMYYSTD 9
            KQAMY STD
Sbjct: 3892 KQAMYSSTD 3900
>NP_001714
          Length = 2649

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KQAMYYS 7
            KQA+YYS
Sbjct: 2298 KQALYYS 2304
>XP_005249383
          Length = 3186

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KQAMYYS 7
            KQA+YYS
Sbjct: 2835 KQALYYS 2841
>NP_001255981
          Length = 404

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KQAMYYS 7
           KQA YYS
Sbjct: 385 KQAFYYS 391
>NP_001255980
          Length = 404

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KQAMYYS 7
           KQA YYS
Sbjct: 385 KQAFYYS 391
>NP_665803
          Length = 1025

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KQAMYYSTD 9
           ++AMYYS +
Sbjct: 718 QEAMYYSAE 726
>NP_001166094
          Length = 1408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MYYSTD 9
           +YYSTD
Sbjct: 992 IYYSTD 997
>NP_002490
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MYYSTD 9
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254473
          Length = 1408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MYYSTD 9
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254472
          Length = 1428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MYYSTD 9
            +YYSTD
Sbjct: 1012 IYYSTD 1017
>XP_005254471
          Length = 1450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MYYSTD 9
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254470
          Length = 1454

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MYYSTD 9
            +YYSTD
Sbjct: 996  IYYSTD 1001
>XP_005254469
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MYYSTD 9
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254468
          Length = 1465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MYYSTD 9
            +YYSTD
Sbjct: 996  IYYSTD 1001
>XP_005254467
          Length = 1470

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MYYSTD 9
            +YYSTD
Sbjct: 1012 IYYSTD 1017
>XP_005254465
          Length = 1481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MYYSTD 9
            +YYSTD
Sbjct: 1012 IYYSTD 1017
>NP_001166095
          Length = 1450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MYYSTD 9
           +YYSTD
Sbjct: 992 IYYSTD 997
>NP_068781
          Length = 859

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           +  +Y+STD
Sbjct: 850 RNTLYFSTD 858
>NP_065101
          Length = 479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           ++ +YY TD
Sbjct: 132 RKKLYYDTD 140
>NP_001153218
          Length = 813

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           +  +Y+STD
Sbjct: 804 RNTLYFSTD 812
>NP_057636
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 646 YYSTD 650
>NP_001269710
          Length = 702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 407 YYSTD 411
>NP_001269709
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 505 YYSTD 509
>NP_001269700
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 735 YYSTD 739
>NP_001243776
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           +  +Y+STD
Sbjct: 301 RNTLYFSTD 309
>NP_001269078
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           +  +Y+STD
Sbjct: 632 RNTLYFSTD 640
>NP_001083
          Length = 822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           +  +Y+STD
Sbjct: 813 RNTLYFSTD 821
>XP_006725355
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KQAMYYSTD 9
           +  +Y+STD
Sbjct: 862 RNTLYFSTD 870
>XP_006721570
          Length = 1118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    KQAMYYSTD 9
            +  +Y+STD
Sbjct: 1109 RNTLYFSTD 1117
>XP_006712635
          Length = 610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 315 YYSTD 319
>XP_006712634
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 408 YYSTD 412
>XP_006712633
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 416 YYSTD 420
>XP_006712632
          Length = 764

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 505 YYSTD 509
>XP_005247077
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 483 YYSTD 487
>XP_006712631
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 505 YYSTD 509
>XP_006712630
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YYSTD 9
           YYSTD
Sbjct: 646 YYSTD 650
>NP_078853
          Length = 1288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    KQAMYYS 7
            K+A+YY+
Sbjct: 1204 KEALYYA 1210
>NP_001018082
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KQAMYYSTD 9
           ++AMY+S +
Sbjct: 796 REAMYHSAE 804
>NP_001017523
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KQAMYYSTD 9
           ++AMY+S +
Sbjct: 333 REAMYHSAE 341
>NP_001193762
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QAMYYSTD 9
           + +YY TD
Sbjct: 308 KTLYYDTD 315
>NP_874369
          Length = 546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QAMYYSTD 9
           + +YY TD
Sbjct: 360 KTLYYDTD 367
>NP_006379
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QAMYYSTD 9
           + +YY TD
Sbjct: 327 KTLYYDTD 334
>NP_874368
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QAMYYSTD 9
           + +YY TD
Sbjct: 275 KTLYYDTD 282
>NP_057363
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   AMYYST 8
           AMYY T
Sbjct: 489 AMYYET 494
>XP_005268702
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KQAMYYSTD 9
           ++AMY+S +
Sbjct: 419 REAMYHSAE 427
>XP_006718484
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QAMYYSTD 9
           + +YY TD
Sbjct: 336 KTLYYDTD 343
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.116    0.321 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 458,791
Number of extensions: 601
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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