Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275707                                                           18    1935
NP_001275705                                                           18    1935
NP_004415                                                              18    1935
NP_006323                                                              18    1935
XP_006720921                                                           18    1935
XP_006720920                                                           18    1935
XP_005255213                                                           18    1935
XP_005255212                                                           18    1935
NP_775760                                                              17    3301
XP_006714502                                                           17    3301
NP_077010                                                              16    5631
NP_005222                                                              16    7355
NP_001265003                                                           16    7355
NP_005236                                                              16    7355
NP_055325                                                              16    7355
NP_149015                                                              16    7355
NP_689600                                                              16    7355
XP_006718884                                                           16    7355
XP_006718882                                                           16    7355
XP_006718511                                                           16    7355
XP_006715989                                                           16    7355
XP_006714202                                                           16    7355
XP_005262892                                                           16    7355
XP_005262891                                                           16    7355
XP_005246358                                                           16    7355
NP_001077073                                                           15    9605
NP_001077075                                                           15    9605
NP_001077076                                                           15    9605
NP_001077072                                                           15    9605
NP_001077071                                                           15    9605
NP_000255                                                              15    9605
NP_001077074                                                           15    9605
NP_001093581                                                           15    9605
NP_061004                                                              15    9605
NP_003293                                                              15    9605
NP_071394                                                              15    9605
NP_001166160                                                           15    9605
NP_001177410                                                           15    9605
NP_001267730                                                           15    9605
NP_001166162                                                           15    9605
NP_060904                                                              15    9605
NP_671724                                                              15    9605
NP_001166163                                                           15    9605
NP_689573                                                              15    9605
XP_005252159                                                           15    9605
XP_006714953                                                           15    9605
NP_001166161                                                           15    9605
NP_060228                                                              15    9605
>NP_001275707
          Length = 607

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 199 KTFKTGSIL 207
>NP_001275705
          Length = 654

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 199 KTFKTGSIL 207
>NP_004415
          Length = 784

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 199 KTFKTGSIL 207
>NP_006323
          Length = 250

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  NYKTGSL 8
          NYKTG+L
Sbjct: 42 NYKTGNL 48
>XP_006720921
          Length = 783

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 199 KTFKTGSIL 207
>XP_006720920
          Length = 785

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 200 KTFKTGSIL 208
>XP_005255213
          Length = 836

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 252 KTFKTGSIL 260
>XP_005255212
          Length = 837

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           K +KTGS+L
Sbjct: 252 KTFKTGSIL 260
>NP_775760
          Length = 1585

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           +N+K GSLL
Sbjct: 924 ENFKIGSLL 932
>XP_006714502
          Length = 1064

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           +N+K GSLL
Sbjct: 407 ENFKIGSLL 415
>NP_077010
          Length = 488

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   YKTGSLL 9
           YK GSLL
Sbjct: 341 YKAGSLL 347
>NP_005222
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KNYKTG 6
           K+YKTG
Sbjct: 263 KDYKTG 268
>NP_001265003
          Length = 1838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    YKTGSLL 9
            YK GSLL
Sbjct: 1707 YKRGSLL 1713
>NP_005236
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NYKTGSL 8
            +YKTG+L
Sbjct: 1930 DYKTGAL 1936
>NP_055325
          Length = 4935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KNYKTGSL 8
            K YK GSL
Sbjct: 3558 KTYKGGSL 3565
>NP_149015
          Length = 5142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KNYKTGSL 8
            K YK GSL
Sbjct: 3558 KTYKGGSL 3565
>NP_689600
          Length = 426

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   NYKTG 6
           NYKTG
Sbjct: 268 NYKTG 272
>XP_006718884
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KNYKTGSLL 9
           K YK+G +L
Sbjct: 129 KQYKSGRIL 137
>XP_006718882
          Length = 182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KNYKTGSLL 9
           K YK+G +L
Sbjct: 129 KQYKSGRIL 137
>XP_006718511
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KNYKTG 6
           K+YKTG
Sbjct: 263 KDYKTG 268
>XP_006715989
          Length = 5148

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KNYKTGSL 8
            K YK GSL
Sbjct: 3558 KTYKGGSL 3565
>XP_006714202
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NYKTGSL 8
            +YKTG+L
Sbjct: 1930 DYKTGAL 1936
>XP_005262892
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NYKTGSL 8
            +YKTG+L
Sbjct: 1930 DYKTGAL 1936
>XP_005262891
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NYKTGSL 8
            +YKTG+L
Sbjct: 1930 DYKTGAL 1936
>XP_005246358
          Length = 426

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   NYKTG 6
           NYKTG
Sbjct: 268 NYKTG 272
>NP_001077073
          Length = 1296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 721 NYKNGS 726
>NP_001077075
          Length = 1296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 721 NYKNGS 726
>NP_001077076
          Length = 1296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 721 NYKNGS 726
>NP_001077072
          Length = 1446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 871 NYKNGS 876
>NP_001077071
          Length = 1381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 806 NYKNGS 811
>NP_000255
          Length = 1447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 872 NYKNGS 877
>NP_001077074
          Length = 1296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 721 NYKNGS 726
>NP_001093581
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  NYKTGSLL 9
          +YKTG ++
Sbjct: 56 SYKTGEMI 63
>NP_061004
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KNYKTGSLL 9
           +N+K G+LL
Sbjct: 810 ENFKGGTLL 818
>NP_003293
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YKTGSL 8
           Y+TGSL
Sbjct: 131 YRTGSL 136
>NP_071394
          Length = 1339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGSLL 9
            KTGSLL
Sbjct: 1251 KTGSLL 1256
>NP_001166160
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNYKTGSLL 9
           K Y TGS L
Sbjct: 305 KTYTTGSKL 313
>NP_001177410
          Length = 1275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGSLL 9
            KTGSLL
Sbjct: 1187 KTGSLL 1192
>NP_001267730
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KNYKTGSLL 9
           KN+  GSL+
Sbjct: 188 KNFSRGSLV 196
>NP_001166162
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNYKTGSLL 9
           K Y TGS L
Sbjct: 391 KTYTTGSKL 399
>NP_060904
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KNYKTGSLL 9
           KN+  GSL+
Sbjct: 188 KNFSRGSLV 196
>NP_671724
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   YKTGSLL 9
           Y+TGS++
Sbjct: 295 YRTGSIM 301
>NP_001166163
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNYKTGSLL 9
           K Y TGS L
Sbjct: 305 KTYTTGSKL 313
>NP_689573
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNYKTGSLL 9
           K Y TGS L
Sbjct: 335 KTYTTGSKL 343
>XP_005252159
          Length = 1296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   NYKTGS 7
           NYK GS
Sbjct: 721 NYKNGS 726
>XP_006714953
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KNYKTGSLL 9
           KN+  GSL+
Sbjct: 188 KNFSRGSLV 196
>NP_001166161
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNYKTGSLL 9
           K Y TGS L
Sbjct: 393 KTYTTGSKL 401
>NP_060228
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2  NYKTGS 7
          NYK GS
Sbjct: 22 NYKAGS 27
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.128    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 643,343
Number of extensions: 832
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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