Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     299
NP_001035957                                                           20     299
XP_006721991                                                           20     299
XP_006721990                                                           20     299
XP_006721989                                                           20     299
XP_005258041                                                           20     299
XP_006721988                                                           20     299
XP_006721987                                                           20     299
XP_006721986                                                           20     299
XP_005258040                                                           20     299
XP_006721985                                                           20     299
XP_006710508                                                           17    2528
XP_006710507                                                           17    2528
NP_689910                                                              17    2528
NP_001123530                                                           17    2528
NP_006659                                                              17    4312
XP_005267331                                                           17    4312
NP_000548                                                              16    5631
NP_659436                                                              16    5631
NP_008949                                                              16    5631
XP_006722338                                                           16    5631
XP_006717007                                                           16    5631
XP_006717006                                                           16    5631
XP_006717005                                                           16    5631
XP_006717004                                                           16    5631
XP_006717003                                                           16    5631
XP_005251736                                                           16    5631
NP_005912                                                              16    7355
NP_001001557                                                           16    7355
NP_002729                                                              16    7355
NP_997700                                                              16    7355
NP_036364                                                              16    7355
NP_004648                                                              16    7355
XP_005257299                                                           16    7355
XP_005248577                                                           16    7355
XP_005248576                                                           16    7355
NP_061720                                                              16    7355
NP_058649                                                              15    9605
NP_060391                                                              15    9605
NP_055178                                                              15    9605
NP_872302                                                              15    9605
NP_001264984                                                           15    9605
NP_060358                                                              15    9605
XP_006721119                                                           15    9605
XP_006721118                                                           15    9605
XP_005266395                                                           15    9605
XP_006719293                                                           15    9605
XP_006715527                                                           15    9605
>NP_000258
          Length = 2818

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 938 KNTISKFFD 946
>NP_001035957
          Length = 2839

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 938 KNTISKFFD 946
>XP_006721991
          Length = 1632

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 948 KNTISKFFD 956
>XP_006721990
          Length = 2823

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 948 KNTISKFFD 956
>XP_006721989
          Length = 2823

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 948 KNTISKFFD 956
>XP_005258041
          Length = 2836

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 938 KNTISKFFD 946
>XP_006721988
          Length = 2846

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 948 KNTISKFFD 956
>XP_006721987
          Length = 2849

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 948 KNTISKFFD 956
>XP_006721986
          Length = 2854

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 935 KNTISKFFD 943
>XP_005258040
          Length = 2857

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 938 KNTISKFFD 946
>XP_006721985
          Length = 2867

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNTISKFLD 9
           KNTISKF D
Sbjct: 948 KNTISKFFD 956
>XP_006710508
          Length = 677

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KNTISKFL 8
           +NT+SKFL
Sbjct: 502 QNTLSKFL 509
>XP_006710507
          Length = 719

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KNTISKFL 8
           +NT+SKFL
Sbjct: 544 QNTLSKFL 551
>NP_689910
          Length = 719

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KNTISKFL 8
           +NT+SKFL
Sbjct: 544 QNTLSKFL 551
>NP_001123530
          Length = 717

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KNTISKFL 8
           +NT+SKFL
Sbjct: 544 QNTLSKFL 551
>NP_006659
          Length = 500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KNTISKFL 8
           +NT++KFL
Sbjct: 226 RNTLAKFL 233
>XP_005267331
          Length = 355

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  KNTISKFL 8
          +NT++KFL
Sbjct: 81 RNTLAKFL 88
>NP_000548
          Length = 501

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTISKFLD 9
           NTI+ F+D
Sbjct: 200 NTITSFID 207
>NP_659436
          Length = 670

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NTISKFLD 9
          NT+ +FLD
Sbjct: 85 NTVLRFLD 92
>NP_008949
          Length = 2325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    KNTISKFLD 9
            KN + KF D
Sbjct: 1457 KNAVEKFTD 1465
>XP_006722338
          Length = 473

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NTISKFLD 9
          NT+ +FLD
Sbjct: 85 NTVLRFLD 92
>XP_006717007
          Length = 1499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    KNTISKFLD 9
            KN + KF D
Sbjct: 1457 KNAVEKFTD 1465
>XP_006717006
          Length = 1760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KNTISKFLD 9
           KN + KF D
Sbjct: 877 KNAVEKFTD 885
>XP_006717005
          Length = 1788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KNTISKFLD 9
           KN + KF D
Sbjct: 905 KNAVEKFTD 913
>XP_006717004
          Length = 2240

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    KNTISKFLD 9
            KN + KF D
Sbjct: 1457 KNAVEKFTD 1465
>XP_006717003
          Length = 2278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    KNTISKFLD 9
            KN + KF D
Sbjct: 1457 KNAVEKFTD 1465
>XP_005251736
          Length = 2325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    KNTISKFLD 9
            KN + KF D
Sbjct: 1457 KNAVEKFTD 1465
>NP_005912
          Length = 1512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KNTISKFL 8
           +NTI KF+
Sbjct: 405 RNTIQKFV 412
>NP_001001557
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTISKFLD 9
           NTI+ F+D
Sbjct: 125 NTITSFVD 132
>NP_002729
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTISKF 7
           NT+SKF
Sbjct: 323 NTVSKF 328
>NP_997700
          Length = 671

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTISKF 7
           NT+SKF
Sbjct: 323 NTVSKF 328
>NP_036364
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTISKFLD 9
           NT+SK L+
Sbjct: 105 NTVSKLLE 112
>NP_004648
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTISKFLD 9
           NT+SK L+
Sbjct: 109 NTVSKLLE 116
>XP_005257299
          Length = 225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTISKFLD 9
           NT+SK L+
Sbjct: 105 NTVSKLLE 112
>XP_005248577
          Length = 1349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KNTISKFL 8
           +NTI KF+
Sbjct: 242 RNTIQKFV 249
>XP_005248576
          Length = 1375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KNTISKFL 8
           +NTI KF+
Sbjct: 268 RNTIQKFV 275
>NP_061720
          Length = 4024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTISKFLD 9
           +T+S FLD
Sbjct: 871 STVSSFLD 878
>NP_058649
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KNTISKFLD 9
           K  +SKF D
Sbjct: 251 KTLVSKFFD 259
>NP_060391
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNTISKFLD 9
           +NT S FLD
Sbjct: 494 QNTSSVFLD 502
>NP_055178
          Length = 4579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KNTISKFL 8
            K+T+ KFL
Sbjct: 2953 KDTLKKFL 2960
>NP_872302
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KNTISKF 7
           +NTI KF
Sbjct: 130 RNTIKKF 136
>NP_001264984
          Length = 4432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KNTISKFL 8
            K+T+ KFL
Sbjct: 2806 KDTLKKFL 2813
>NP_060358
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTISKF 7
           NT+SKF
Sbjct: 307 NTLSKF 312
>XP_006721119
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTISKF 7
           NT+SKF
Sbjct: 307 NTLSKF 312
>XP_006721118
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NTISKF 7
           NT+SKF
Sbjct: 307 NTLSKF 312
>XP_005266395
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KNTISKFL 8
            K+T+ KFL
Sbjct: 2962 KDTLKKFL 2969
>XP_006719293
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KNTISKF 7
           +NTI KF
Sbjct: 130 RNTIKKF 136
>XP_006715527
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NTISKFLD 9
           +TIS+FL+
Sbjct: 266 STISRFLE 273
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.133    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,410
Number of extensions: 598
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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