Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              19     869
XP_005271924                                                           19     869
XP_005271923                                                           19     869
NP_003600                                                              17    3301
NP_443161                                                              17    4312
XP_006717206                                                           17    4312
XP_005252108                                                           17    4312
XP_006710380                                                           17    4312
XP_005245788                                                           17    4312
XP_006710379                                                           17    4312
XP_006710378                                                           17    4312
NP_005383                                                              17    4312
NP_001035203                                                           16    7355
NP_074035                                                              16    7355
NP_001035202                                                           16    7355
NP_002465                                                              16    7355
NP_000858                                                              15    9605
NP_038478                                                              15    9605
NP_001276904                                                           15    9605
NP_056052                                                              15    9605
NP_443143                                                              15    9605
NP_001059                                                              15    9605
XP_006722442                                                           15    9605
XP_005268117                                                           15    9605
XP_005265484                                                           15    9605
XP_005246577                                                           15    9605
XP_006712545                                                           15    9605
XP_006712537                                                           15    9605
XP_005246557                                                           15    9605
XP_006712535                                                           15    9605
XP_006712532                                                           15    9605
XP_006712531                                                           15    9605
XP_006712530                                                           15    9605
XP_006712529                                                           15    9605
XP_006712528                                                           15    9605
XP_006712527                                                           15    9605
XP_005246549                                                           15    9605
XP_006712526                                                           15    9605
XP_006712525                                                           15    9605
XP_005246546                                                           15    9605
XP_006712524                                                           15    9605
XP_005246545                                                           15    9605
>NP_001261
          Length = 1710

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KNKATKSIK 9
            KNKA KSIK
Sbjct: 1338 KNKAMKSIK 1346
>XP_005271924
          Length = 1710

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KNKATKSIK 9
            KNKA KSIK
Sbjct: 1338 KNKAMKSIK 1346
>XP_005271923
          Length = 1798

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KNKATKSIK 9
            KNKA KSIK
Sbjct: 1338 KNKAMKSIK 1346
>NP_003600
          Length = 556

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NKATKSIK 9
           NK TKS+K
Sbjct: 214 NKGTKSLK 221
>NP_443161
          Length = 1412

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNKATKSIK 9
           K KAT+S+K
Sbjct: 860 KTKATESLK 868
>XP_006717206
          Length = 1062

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KNKATKSI 8
           K KATKS+
Sbjct: 529 KGKATKSV 536
>XP_005252108
          Length = 1097

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KNKATKSI 8
           K KATKS+
Sbjct: 564 KGKATKSV 571
>XP_006710380
          Length = 1398

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNKATKSIK 9
           K KAT+S+K
Sbjct: 846 KTKATESLK 854
>XP_005245788
          Length = 1434

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNKATKSIK 9
           K KAT+S+K
Sbjct: 882 KTKATESLK 890
>XP_006710379
          Length = 1452

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNKATKSIK 9
           K KAT+S+K
Sbjct: 882 KTKATESLK 890
>XP_006710378
          Length = 1478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNKATKSIK 9
           K KAT+S+K
Sbjct: 882 KTKATESLK 890
>NP_005383
          Length = 1096

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KNKATKSI 8
           K KATKS+
Sbjct: 564 KGKATKSV 571
>NP_001035203
          Length = 1979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    KNKATKSIK 9
            K  ATKS+K
Sbjct: 1844 KQAATKSLK 1852
>NP_074035
          Length = 1938

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    KNKATKSIK 9
            K  ATKS+K
Sbjct: 1837 KQAATKSLK 1845
>NP_001035202
          Length = 1945

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    KNKATKSIK 9
            K  ATKS+K
Sbjct: 1844 KQAATKSLK 1852
>NP_002465
          Length = 1972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    KNKATKSIK 9
            K  ATKS+K
Sbjct: 1837 KQAATKSLK 1845
>NP_000858
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KATKSIK 9
           +ATKS+K
Sbjct: 400 RATKSVK 406
>NP_038478
          Length = 2168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>NP_001276904
          Length = 2132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 671 NKTTSSVK 678
>NP_056052
          Length = 1354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KNKATKSI 8
            KN +TKS+
Sbjct: 1161 KNSSTKSL 1168
>NP_443143
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  NKATKSI 8
          +KATKSI
Sbjct: 77 DKATKSI 83
>NP_001059
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNKATKSIK 9
           KNKA  S+K
Sbjct: 360 KNKAGVSVK 368
>XP_006722442
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  NKATKSI 8
          +KATKSI
Sbjct: 77 DKATKSI 83
>XP_005268117
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NKATKSIK 9
           NKA +SIK
Sbjct: 230 NKADESIK 237
>XP_005265484
          Length = 1626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNKATKSIK 9
           KNKA  S+K
Sbjct: 365 KNKAGVSVK 373
>XP_005246577
          Length = 362

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KATKSIK 9
           +ATKS+K
Sbjct: 281 RATKSVK 287
>XP_006712545
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KATKSIK 9
           +ATKS+K
Sbjct: 375 RATKSVK 381
>XP_006712537
          Length = 1952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_005246557
          Length = 2095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 554 NKTTSSVK 561
>XP_006712535
          Length = 2095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 554 NKTTSSVK 561
>XP_006712532
          Length = 2141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 671 NKTTSSVK 678
>XP_006712531
          Length = 2169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_006712530
          Length = 2176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 671 NKTTSSVK 678
>XP_006712529
          Length = 2179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_006712528
          Length = 2180

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_006712527
          Length = 2203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_005246549
          Length = 2212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 671 NKTTSSVK 678
>XP_006712526
          Length = 2212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 671 NKTTSSVK 678
>XP_006712525
          Length = 2212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_005246546
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_006712524
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
>XP_005246545
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   NKATKSIK 9
           NK T S+K
Sbjct: 673 NKTTSSVK 680
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.104    0.236 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 317,586
Number of extensions: 412
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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