Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002961                                                              18    1935
NP_112190                                                              16    7355
NP_872393                                                              15    9605
XP_005270205                                                           15    9605
XP_006712515                                                           15    9605
XP_006712514                                                           15    9605
XP_006712513                                                           15    9605
XP_006712512                                                           15    9605
XP_006712511                                                           15    9605
XP_006712510                                                           15    9605
XP_006712509                                                           15    9605
XP_005246533                                                           15    9605
XP_006712508                                                           15    9605
NP_115893                                                              15    9605
NP_076950                                                              15    9605
>NP_002961
          Length = 171

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   KNISQMALR 9
           KN+SQ+A+R
Sbjct: 111 KNLSQVAMR 119
>NP_112190
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNISQMALR 9
           K +SQ+ALR
Sbjct: 377 KALSQLALR 385
>NP_872393
          Length = 3234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1510 NISSVALR 1517
>XP_005270205
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   KNISQMAL 8
           KN+++MA+
Sbjct: 335 KNVTEMAM 342
>XP_006712515
          Length = 3232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1508 NISSVALR 1515
>XP_006712514
          Length = 2496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>XP_006712513
          Length = 3220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1477 NISSVALR 1484
>XP_006712512
          Length = 3237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1513 NISSVALR 1520
>XP_006712511
          Length = 3256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1513 NISSVALR 1520
>XP_006712510
          Length = 3305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>XP_006712509
          Length = 3319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1576 NISSVALR 1583
>XP_005246533
          Length = 3323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>XP_006712508
          Length = 3324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1581 NISSVALR 1588
>NP_115893
          Length = 3258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NISQMALR 9
            NIS +ALR
Sbjct: 1515 NISSVALR 1522
>NP_076950
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   KNISQMAL 8
           KN+++MA+
Sbjct: 400 KNVTEMAM 407
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.124    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 247,100
Number of extensions: 245
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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