Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              19     665
XP_005251512                                                           19     665
NP_001561                                                              18    1935
NP_008835                                                              17    2528
NP_001075109                                                           17    2528
NP_005126                                                              17    3301
NP_598408                                                              17    3301
NP_001035959                                                           17    3301
NP_001035962                                                           17    3301
NP_001035960                                                           17    3301
NP_001035961                                                           17    3301
XP_006720856                                                           17    3301
NP_640339                                                              17    4312
NP_065759                                                              17    4312
NP_001128243                                                           17    4312
NP_006589                                                              17    4312
NP_006132                                                              17    4312
NP_001273086                                                           17    4312
XP_006725069                                                           17    4312
XP_006717042                                                           17    4312
XP_005251797                                                           17    4312
XP_005248288                                                           17    4312
NP_835227                                                              16    5631
NP_067045                                                              16    5631
XP_005245451                                                           16    5631
XP_005245450                                                           16    5631
NP_659477                                                              16    5631
NP_001073291                                                           16    7355
NP_000417                                                              16    7355
NP_060040                                                              16    7355
NP_001153101                                                           16    7355
NP_003636                                                              16    7355
NP_001136089                                                           16    7355
NP_001185970                                                           16    7355
NP_001185971                                                           16    7355
NP_001185969                                                           16    7355
NP_001136091                                                           16    7355
XP_005267039                                                           16    7355
XP_005267038                                                           16    7355
NP_001136090                                                           16    7355
NP_071764                                                              16    7355
NP_001001712                                                           16    7355
NP_055448                                                              16    7355
NP_037368                                                              15    9605
NP_001161093                                                           15    9605
NP_075048                                                              15    9605
NP_055839                                                              15    9605
>NP_004963
          Length = 1132

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNILLFREE 9
           KNILL REE
Sbjct: 677 KNILLIREE 685
>XP_005251512
          Length = 1132

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KNILLFREE 9
           KNILL REE
Sbjct: 677 KNILLIREE 685
>NP_001561
          Length = 625

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   KNILLFREE 9
           +NILL++EE
Sbjct: 607 ENILLYKEE 615
>NP_008835
          Length = 4128

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KNILLFREE 9
            KNIL FR++
Sbjct: 3318 KNILAFRDQ 3326
>NP_001075109
          Length = 4097

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KNILLFREE 9
            KNIL FR++
Sbjct: 3318 KNILAFRDQ 3326
>NP_005126
          Length = 1099

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 80 KNLALFEEE 88
>NP_598408
          Length = 1150

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNILLFREE 9
           KN+ LF EE
Sbjct: 131 KNLALFEEE 139
>NP_001035959
          Length = 1091

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 72 KNLALFEEE 80
>NP_001035962
          Length = 1135

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNILLFREE 9
           KN+ LF EE
Sbjct: 116 KNLALFEEE 124
>NP_001035960
          Length = 1091

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 72 KNLALFEEE 80
>NP_001035961
          Length = 1141

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNILLFREE 9
           KN+ LF EE
Sbjct: 122 KNLALFEEE 130
>XP_006720856
          Length = 1101

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNILLFREE 9
           KN+ LF EE
Sbjct: 131 KNLALFEEE 139
>NP_640339
          Length = 516

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNILLFREE 9
           KN+ LFRE+
Sbjct: 341 KNLELFREK 349
>NP_065759
          Length = 1116

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 43 KNMALFEEE 51
>NP_001128243
          Length = 1139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 66 KNMALFEEE 74
>NP_006589
          Length = 1083

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 67 KNMALFEEE 75
>NP_006132
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KNILLFREE 9
          KNIL+F E+
Sbjct: 55 KNILVFGED 63
>NP_001273086
          Length = 415

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KNILLFREE 9
          KNIL+F E+
Sbjct: 55 KNILVFGED 63
>XP_006725069
          Length = 1088

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 67 KNMALFEEE 75
>XP_006717042
          Length = 290

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNILLFREE 9
           KN+ LFRE+
Sbjct: 115 KNLELFREK 123
>XP_005251797
          Length = 475

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KNILLFREE 9
           KN+ LFRE+
Sbjct: 300 KNLELFREK 308
>XP_005248288
          Length = 1088

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          KN+ LF EE
Sbjct: 67 KNMALFEEE 75
>NP_835227
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   KNILLFREE 9
           +N+LLF+++
Sbjct: 156 RNVLLFKKQ 164
>NP_067045
          Length = 453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  KNILLFREE 9
          ++IL+FR+E
Sbjct: 80 ESILIFRDE 88
>XP_005245451
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  KNILLFREE 9
          ++IL+FR+E
Sbjct: 80 ESILIFRDE 88
>XP_005245450
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  KNILLFREE 9
          ++IL+FR+E
Sbjct: 50 ESILIFRDE 58
>NP_659477
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NILLFREE 9
           ++LLF+EE
Sbjct: 123 HVLLFKEE 130
>NP_001073291
          Length = 3118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NILLFREE 9
           N+LL +EE
Sbjct: 651 NVLLLKEE 658
>NP_000417
          Length = 3122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NILLFREE 9
           N+LL +EE
Sbjct: 651 NVLLLKEE 658
>NP_060040
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    LLFREE 9
            LLFREE
Sbjct: 1184 LLFREE 1189
>NP_001153101
          Length = 567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          K  LLFR+E
Sbjct: 69 KPFLLFRDE 77
>NP_003636
          Length = 620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNILLFREE 9
          K  LLFR+E
Sbjct: 69 KPFLLFRDE 77
>NP_001136089
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 461 RNLLLFR 467
>NP_001185970
          Length = 682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 476 RNLLLFR 482
>NP_001185971
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 500 RNLLLFR 506
>NP_001185969
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 498 RNLLLFR 504
>NP_001136091
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 452 RNLLLFR 458
>XP_005267039
          Length = 3206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NILLFREE 9
           N+LL +EE
Sbjct: 651 NVLLLKEE 658
>XP_005267038
          Length = 3210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NILLFREE 9
           N+LL +EE
Sbjct: 651 NVLLLKEE 658
>NP_001136090
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 461 RNLLLFR 467
>NP_071764
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KNILLFR 7
           +N+LLFR
Sbjct: 461 RNLLLFR 467
>NP_001001712
          Length = 200

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KNILLFREE 9
           KN+LLF  +
Sbjct: 153 KNLLLFHRQ 161
>NP_055448
          Length = 1173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNILLFREE 9
           KNIL+F  E
Sbjct: 886 KNILMFTGE 894
>NP_037368
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ILLFRE 8
           ILLFRE
Sbjct: 115 ILLFRE 120
>NP_001161093
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ILLFRE 8
          ILLFRE
Sbjct: 51 ILLFRE 56
>NP_075048
          Length = 1088

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ILLFRE 8
           ILLFRE
Sbjct: 794 ILLFRE 799
>NP_055839
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ILLFRE 8
           ILLFRE
Sbjct: 796 ILLFRE 801
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.149    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 480,562
Number of extensions: 813
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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