Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1482
NP_005913                                                              18    1482
XP_005267046                                                           18    1482
XP_005267045                                                           18    1482
NP_001181966                                                           17    2528
NP_001071174                                                           17    2528
XP_006715535                                                           17    2528
NP_001186184                                                           17    4312
NP_001186182                                                           17    4312
NP_001186180                                                           17    4312
NP_001012411                                                           17    4312
NP_001012409                                                           17    4312
NP_001012413                                                           17    4312
NP_001012410                                                           17    4312
NP_001186185                                                           17    4312
NP_612493                                                              17    4312
NP_001012412                                                           17    4312
NP_001186181                                                           17    4312
NP_001186183                                                           17    4312
XP_006713050                                                           17    4312
XP_006713049                                                           17    4312
XP_005246719                                                           16    5631
XP_005246718                                                           16    5631
NP_060556                                                              16    5631
NP_067641                                                              16    7355
NP_001159432                                                           16    7355
NP_004516                                                              16    7355
NP_000607                                                              16    7355
NP_006763                                                              16    7355
NP_892121                                                              16    7355
NP_056311                                                              16    7355
XP_005256802                                                           16    7355
XP_006712591                                                           16    7355
XP_006712590                                                           16    7355
XP_006712589                                                           16    7355
NP_061834                                                              15    9605
NP_001001895                                                           15    9605
XP_006724076                                                           15    9605
>NP_006715
          Length = 1558

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KMRCTASQL 9
            KMR TASQL
Sbjct: 1537 KMRWTASQL 1545
>NP_005913
          Length = 1608

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KMRCTASQL 9
            KMR TASQL
Sbjct: 1587 KMRWTASQL 1595
>XP_005267046
          Length = 1554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KMRCTASQL 9
            KMR TASQL
Sbjct: 1533 KMRWTASQL 1541
>XP_005267045
          Length = 1604

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KMRCTASQL 9
            KMR TASQL
Sbjct: 1583 KMRWTASQL 1591
>NP_001181966
          Length = 904

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  MRCTASQL 9
          +RCT SQL
Sbjct: 52 LRCTKSQL 59
>NP_001071174
          Length = 904

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  MRCTASQL 9
          +RCT SQL
Sbjct: 52 LRCTKSQL 59
>XP_006715535
          Length = 946

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  MRCTASQL 9
          +RCT SQL
Sbjct: 52 LRCTKSQL 59
>NP_001186184
          Length = 258

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 206 KRRCTAS 212
>NP_001186182
          Length = 275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 223 KRRCTAS 229
>NP_001186180
          Length = 527

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 475 KRRCTAS 481
>NP_001012411
          Length = 275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 223 KRRCTAS 229
>NP_001012409
          Length = 527

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 475 KRRCTAS 481
>NP_001012413
          Length = 258

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 206 KRRCTAS 212
>NP_001012410
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 475 KRRCTAS 481
>NP_001186185
          Length = 292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 206 KRRCTAS 212
>NP_612493
          Length = 292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 206 KRRCTAS 212
>NP_001012412
          Length = 309

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 223 KRRCTAS 229
>NP_001186181
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 475 KRRCTAS 481
>NP_001186183
          Length = 309

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 223 KRRCTAS 229
>XP_006713050
          Length = 527

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 475 KRRCTAS 481
>XP_006713049
          Length = 527

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KMRCTAS 7
           K RCTAS
Sbjct: 475 KRRCTAS 481
>XP_005246719
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RCTASQL 9
           RC ASQL
Sbjct: 526 RCIASQL 532
>XP_005246718
          Length = 785

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RCTASQL 9
           RC ASQL
Sbjct: 563 RCIASQL 569
>NP_060556
          Length = 759

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RCTASQL 9
           RC ASQL
Sbjct: 537 RCIASQL 543
>NP_067641
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  KMRCTA 6
          K+RCTA
Sbjct: 54 KVRCTA 59
>NP_001159432
          Length = 843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KMRCTA 6
           K+RCTA
Sbjct: 186 KVRCTA 191
>NP_004516
          Length = 4655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MRCTASQ 8
            + CTASQ
Sbjct: 1185 LNCTASQ 1191
>NP_000607
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  KMRCTASQ 8
          ++ CTASQ
Sbjct: 38 ELTCTASQ 45
>NP_006763
          Length = 1343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MRCTASQL 9
           ++CTAS L
Sbjct: 529 IKCTASSL 536
>NP_892121
          Length = 1147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RCTASQ 8
           RCTAS+
Sbjct: 780 RCTASE 785
>NP_056311
          Length = 1812

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RCTASQ 8
           RCTAS+
Sbjct: 780 RCTASE 785
>XP_005256802
          Length = 867

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KMRCTA 6
           K+RCTA
Sbjct: 210 KVRCTA 215
>XP_006712591
          Length = 3308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MRCTASQ 8
            + CTASQ
Sbjct: 1185 LNCTASQ 1191
>XP_006712590
          Length = 3897

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   MRCTASQ 8
           + CTASQ
Sbjct: 422 LNCTASQ 428
>XP_006712589
          Length = 4660

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MRCTASQ 8
            + CTASQ
Sbjct: 1185 LNCTASQ 1191
>NP_061834
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RCTASQL 9
           RCTAS +
Sbjct: 564 RCTASMV 570
>NP_001001895
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RCTASQL 9
           RCTAS +
Sbjct: 526 RCTASMV 532
>XP_006724076
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RCTASQL 9
           RCTAS +
Sbjct: 545 RCTASMV 551
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.119    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,347
Number of extensions: 278
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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