Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001277251                                                           18    1935
NP_005500                                                              18    1935
NP_001277250                                                           18    1935
XP_005271970                                                           18    1935
XP_005271969                                                           18    1935
XP_005271967                                                           18    1935
XP_005271966                                                           18    1935
NP_056078                                                              17    2528
NP_001167587                                                           17    2528
NP_001167588                                                           17    2528
XP_005254315                                                           17    2528
XP_005254314                                                           17    2528
XP_006720509                                                           17    2528
XP_005254313                                                           17    2528
XP_005254312                                                           17    2528
NP_001277002                                                           16    5631
NP_067547                                                              16    5631
NP_003390                                                              16    5631
NP_001277001                                                           16    5631
XP_005249317                                                           16    5631
NP_002988                                                              16    7355
NP_001006947                                                           16    7355
NP_001028690                                                           16    7355
NP_056425                                                              16    7355
NP_001243689                                                           16    7355
NP_000859                                                              16    7355
NP_775760                                                              15    9605
NP_689548                                                              15    9605
NP_001363                                                              15    9605
XP_006721530                                                           15    9605
XP_006721529                                                           15    9605
XP_006721528                                                           15    9605
XP_006714502                                                           15    9605
NP_803875                                                              15    9605
>NP_001277251
          Length = 2854

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1473 MKKKAVIW 1480
>NP_005500
          Length = 3027

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1646 MKKKAVIW 1653
>NP_001277250
          Length = 3048

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1646 MKKKAVIW 1653
>XP_005271970
          Length = 2931

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1646 MKKKAVIW 1653
>XP_005271969
          Length = 3020

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1580 MKKKAVIW 1587
>XP_005271967
          Length = 3065

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1646 MKKKAVIW 1653
>XP_005271966
          Length = 3086

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AVIW
Sbjct: 1646 MKKKAVIW 1653
>NP_056078
          Length = 3036

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>NP_001167587
          Length = 3037

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>NP_001167588
          Length = 2400

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1037 MKKKAVVW 1044
>XP_005254315
          Length = 2576

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>XP_005254314
          Length = 2605

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>XP_006720509
          Length = 2646

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>XP_005254313
          Length = 3057

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>XP_005254312
          Length = 3058

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MMKRAVIW 9
            M K+AV+W
Sbjct: 1673 MKKKAVVW 1680
>NP_001277002
          Length = 383

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KMMKRAVIW 9
           +M+K+A +W
Sbjct: 126 QMLKKAKLW 134
>NP_067547
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KMMKRAVIW 9
           +M+K+A +W
Sbjct: 220 QMLKKAKLW 228
>NP_003390
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MKRAVIW 9
           MK+A +W
Sbjct: 106 MKKAAVW 112
>NP_001277001
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KMMKRAVIW 9
           +M+K+A +W
Sbjct: 220 QMLKKAKLW 228
>XP_005249317
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KMMKRAVIW 9
           +M+K+A +W
Sbjct: 220 QMLKKAKLW 228
>NP_002988
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3 MKRAVIW 9
         M+RA +W
Sbjct: 1 MRRAALW 7
>NP_001006947
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3 MKRAVIW 9
         M+RA +W
Sbjct: 1 MRRAALW 7
>NP_001028690
          Length = 443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4  KRAVIW 9
          +RAV+W
Sbjct: 38 RRAVVW 43
>NP_056425
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4  KRAVIW 9
          +RAV+W
Sbjct: 38 RRAVVW 43
>NP_001243689
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MMKRAVIW 9
           +MK A++W
Sbjct: 172 IMKIAIVW 179
>NP_000859
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MMKRAVIW 9
           +MK A++W
Sbjct: 172 IMKIAIVW 179
>NP_775760
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 7/9 (77%)

Query: 1    KMMKRAVIW 9
            ++MK  ++W
Sbjct: 1288 ELMKEPILW 1296
>NP_689548
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4  KRAVIW 9
          KRA +W
Sbjct: 86 KRAAVW 91
>NP_001363
          Length = 4486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    KMMKRAVIW 9
            ++MKR  +W
Sbjct: 2169 QIMKRRPVW 2177
>XP_006721530
          Length = 2746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    KMMKRAVIW 9
            ++MKR  +W
Sbjct: 2169 QIMKRRPVW 2177
>XP_006721529
          Length = 2817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    KMMKRAVIW 9
            ++MKR  +W
Sbjct: 2169 QIMKRRPVW 2177
>XP_006721528
          Length = 3869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    KMMKRAVIW 9
            ++MKR  +W
Sbjct: 2169 QIMKRRPVW 2177
>XP_006714502
          Length = 1064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 7/9 (77%)

Query: 1   KMMKRAVIW 9
           ++MK  ++W
Sbjct: 771 ELMKEPILW 779
>NP_803875
          Length = 4243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    MKRAVIW 9
            M+R +IW
Sbjct: 3072 MERLIIW 3078
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.130    0.477 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 358,895
Number of extensions: 497
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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